HIF3 alpha (HIF3A) (NM_152796) Human Tagged ORF Clone

CAT#: RG232759

HIF3A (GFP-tagged) - Homo sapiens hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HIF3A
• Synonyms: bHLHe17; HIF-3A; HIF3-alpha-1; IPAS; MOP7; PASD7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232759 representing NM_152796
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCCCGCAGCCGGCGCAGCCAGGAGACCGAGGTGCTGTACCAGCTGGCTCACACGCTGCCCTTCGC
  CCGCGGCGTCAGCGCCCACCTGGACAAGGCCTCTATCATGCGCCTCACCATCAGCTACCTGCGCATGCAC
  CGCCTCTGCGCCGCAGCTGGAGCTCATTGGACACAGCATCTTTGATTTCATCCACCCCTGTGACCAAGAG
  GAGCTTCAGGACGCCCTGACCCCCCAGCAGACCCTGTCCAGGAGGAAGGTGGAGGCCCCCACGGAGCGGT
  GCTTCTCCTTGCGCATGAAGAGTACACTCACCAGCCGCGGGCGCACCCTCAACCTCAAGGCGGCCACCTG
  GAAGGTGCTGAACTGCTCTGGACATATGAGGGCCTACAAGCCACCTGCGCAGACTTCTCCAGCTGGGAGC
  CCTGACTCAGAGCCCCCGCTGCAGTGCCTGGTGCTCATCTGCGAAGCCATCCCCCACCCAGGCAGCCTGG
  AGCCCCCACTGGGCCGAGGGGCCTTCCTCAGCCGCCACAGCCTGGACATGAAGTTCACCTACTGTGACGA
  CAGGATTGCAGAAGTGGCTGGCTATAGTCCCGATGACCTGATCGGCTGTTCCGCCTACGAGTACATCCAC
  GCGCTGGACTCCGATGCGGTCAGCAAGAGCATCCACACCTTGCTGAGCAAGGGCCAGGCAGTAACAGGGC
  AGTATCGCTTCCTGGCCCGGAGTGGTGGCTACCTGTGGACCCAGACCCAGGCCACAGTGGTGTCAGGGGG
  ACGGGGCCCCCAGTCGGAGAGTATCGTCTGTGTCCATTTTTTAATCAGCCAGGTGGAAGAGACCGGAGTG
  GTGCTGTCCCTGGAGCAAACGGAGCAACACTCTCGCAGACCCATTCAGCGGGGCGCCCCCTCTCAGAAGG
  ACACCCCTAACCCTGGGGACAGCCTTGACACCCCTGGCCCCCGGATCCTTGCCTTCCTGCACCCGCCTTC
  CCTGAGCGAGGCTGCCCTGGCCGCTGACCCCCGCCGTTTCTGCAGCCCTGACCTCCGTCGCCTCCTGGGA
  CCCATCCTGGATGGGGCTTCAGTAGCAGCCACTCCCAGCACCCCGCTGGCCACACGGCACCCCCAAAGTC
  CTCTTTCGGCTGATCTCCCAGATGAACTACCTGTGGGCACCGAGAATGTGCACAGACTCTTCACCTCCGG
  GAAAGACACTGAGGCAGTGGAGACAGATTTAGATATAGCTCAGGACCCCAGCACCCCACTCCTGAACCTG
  AATGAGCCCCTGGGTTTTCACTTTGTCACCCAGTCTGGAGTGCAGTGGCACAAACACAGCTCACCGCAGC
  CTCGACCTCCTGGGCTCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232759 representing NM_152796
  Red=Cloning site Green=Tags(s)
  
  MRPAAGAARRPRCCTSWLTRCPSPAASAPTWTRPLSCASPSATCACTASAPQLELIGHSIFDFIHPCDQE
  ELQDALTPQQTLSRRKVEAPTERCFSLRMKSTLTSRGRTLNLKAATWKVLNCSGHMRAYKPPAQTSPAGS
  PDSEPPLQCLVLICEAIPHPGSLEPPLGRGAFLSRHSLDMKFTYCDDRIAEVAGYSPDDLIGCSAYEYIH
  ALDSDAVSKSIHTLLSKGQAVTGQYRFLARSGGYLWTQTQATVVSGGRGPQSESIVCVHFLISQVEETGV
  VLSLEQTEQHSRRPIQRGAPSQKDTPNPGDSLDTPGPRILAFLHPPSLSEAALAADPRRFCSPDLRRLLG
  PILDGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDLDIAQDPSTPLLNL
  NEPLGFHFVTQSGVQWHKHSSPQPRPPGLK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152796
• ORF Size: 1350 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152796.2, NP_690009.1
• RefSeq Size: 1777
• RefSeq ORF: 1353
• Locus ID: 64344
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]