PPP3CC (NM_001243975) Human Tagged ORF Clone

CAT#: RG232872

PPP3CC (GFP-tagged) - Homo sapiens protein phosphatase 3, catalytic subunit, gamma isozyme (PPP3CC), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPP3CC
• Synonyms: CALNA3; CNA3; PP2Bgamma
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232872 representing NM_001243975
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGAGGCGCTTCCACCTCTCCACCACCGACCGCGTCATCAAAGCTGTCCCCTTTCCTCCAACCC
  AACGGCTTACTTTCAAGGAAGTATTTGAGAATGGGAAACCTAAAGTTGATGTTTTAAAAAACCATTTGGT
  AAAGGAAGGACGACTGGAAGAGGAAGTAGCCTTAAAGATAATCAATGATGGGGCTGCCATCCTGAGGCAA
  GAGAAGACTATGATAGAAGTAGATGCTCCAATCACAGTATGTGGTGATATTCATGGACAATTCTTTGACC
  TAATGAAGTTATTTGAAGTTGGAGGATCACCTAGTAACACACGCTACCTCTTTCTGGGTGACTATGTGGA
  CAGAGGCTATTTCAGTATAGAGTGTGTGCTGTATTTATGGAGTTTAAAGATTAATCATCCCAAAACATTG
  TTTCTGCTTCGGGGAAATCATGAATGCAGGCATCTTACAGACTATTTCACCTTCAAACAGGAATGTCGAA
  TCAAATATTCGGAACAGGTGTATGATGCCTGTATGGAGACATTTGACTGTCTTCCTCTTGCTGCCCTCTT
  AAACCAGCAGTTTCTCTGTGTACATGGAGGAATGTCACCTGAAATTACTTCTTTAGATGACATTAGGAAA
  TTAGACAGGTTTACGGAACCTCCCGCCTTTGGACCTGTGTGTGACCTGCTTTGGTCTGATCCCTCAGAGG
  ATTATGGCAATGAGAAGACCTTGGAGCACTATACCCACAACACTGTCCGAGGGTGCTCTTATTTCTACAG
  TTACCCTGCAGTTTGTGAATTTTTGCAGAACAATAATTTACTATCAATTATCAGAGCCCATGAAGCCCAA
  GATGCTGGGTATCGAATGTACAGGAAGAGCCAAGCCACAGGCTTTCCATCACTTATTACAATTTTCTCTG
  CCCCCAATTACCTAGATGTCTATAACAATAAAGCTGCTGTGTTGAAATATGAAAACAATGTCATGAATAT
  CAGGCAGTTTAACTGTTCTCCACACCCCTACTGGCTTCCAAACTTTATGGATGTTTTCACATGGTCTTTG
  CCTTTTGTTGGGGAAAAAGTCACAGAGATGCTGGTAAATGTGCTCAACATATGCTCTGATGACGAACTGA
  TTTCTGATGATGAAGCAGAAGGAAGCACTACAGTTCGTAAGGAGATCATCAGGAATAAGATCAGAGCCAT
  TGGGAAGATGGCACGGGTCTTTTCAATTCTTCGGCAAGAAAGTGAGAGTGTGCTGACTCTCAAGGGCCTG
  ACTCCCACAGGCACACTCCCTCTGGGCGTCCTCTCAGGAGGCAAGCAGACTATCGAGACAGCCATCAGAG
  GGTTCTCGCTTCAGCACAAGATCCGGAGTTTTGAAGAAGCGCGAGGTCTGGACCGAATTAATGAGCGAAT
  GCCACCCCGAAAGGATAGCATACACGCTGGTGGGCCAATGAAATCTGTAACCTCAGCACACTCACATGCT
  GCGCACAGGAGCGACCAAGGGAAGAAAGCCCATTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232872 representing NM_001243975
  Red=Cloning site Green=Tags(s)
  
  MSGRRFHLSTTDRVIKAVPFPPTQRLTFKEVFENGKPKVDVLKNHLVKEGRLEEEVALKIINDGAAILRQ
  EKTMIEVDAPITVCGDIHGQFFDLMKLFEVGGSPSNTRYLFLGDYVDRGYFSIECVLYLWSLKINHPKTL
  FLLRGNHECRHLTDYFTFKQECRIKYSEQVYDACMETFDCLPLAALLNQQFLCVHGGMSPEITSLDDIRK
  LDRFTEPPAFGPVCDLLWSDPSEDYGNEKTLEHYTHNTVRGCSYFYSYPAVCEFLQNNNLLSIIRAHEAQ
  DAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSL
  PFVGEKVTEMLVNVLNICSDDELISDDEAEGSTTVRKEIIRNKIRAIGKMARVFSILRQESESVLTLKGL
  TPTGTLPLGVLSGGKQTIETAIRGFSLQHKIRSFEEARGLDRINERMPPRKDSIHAGGPMKSVTSAHSHA
  AHRSDQGKKAHS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243975
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243975.1, NP_001230904.1
• RefSeq Size: 2304 bp
• RefSeq ORF: 1509 bp
• Locus ID: 5533
• Cytogenetics: 8p21.3
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: 'Calcineurin is a calcium-dependent, calmodulin-stimulated protein phosphatase involved in the downstream regulation of dopaminergic signal transduction. Calcineurin is composed of a regulatory subunit and a catalytic subunit. The protein encoded by this gene represents one of the regulatory subunits that has been found for calcineurin. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]'