SEMA4F (NM_001271661) Human Tagged ORF Clone

CAT#: RG233014

SEMA4F (GFP-tagged) - Homo sapiens sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (SEMA4F), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SEMA4F
• Synonyms: M-SEMA; m-Sema-M; PRO2353; S4F; SEMAM; SEMAW
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233014 representing NM_001271661
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCTCTGCTGCGCGGCCCCGCCCGGGTCCCGGGCAGCCTACAGCCTCGCCCTTCCCGCTACTGC
  TGCTGGCGGTGCTGAGCGGCCCGGTATCCGGCCGCGTCCCCCGCTCGGTGCCCAGAACCTCGCTTCCAAT
  CTCTGAGGCTGACTCCTGTCTCACCCGGTTCGCAGTCCCTCACACATACAATTACTCTGTTCTCCTTGTG
  GATCCTGCCTCCCACACACTTTATGTTGGCGCCCGGGACACCATCTTCGCTTTATCCCTGCCCTTCTCAG
  GGGAGAGACCCCGCAGGATTGACTGGATGGTTCCTGAGGCTCACAGACAGAACTGTAGGAAGAAAGGCAA
  GAAAGAGGGGGACCTCGGGGGCCGGAAGACCCTCCAGCAGAGATGGACGACGTTTTTGAAAGCTGACCTG
  CTCTGTCCAGGGCCTGAGCATGGCCGGGCCTCCAGTGTCCTGCAGGATGTTGCTGTGCTTCGACCTGAGC
  TTGGGGCAGGGACTCCCATCTTTTATGGCATCTTTTCTTCCCAGTGGGAGGGGGCTACTATCTCTGCTGT
  CTGTGCCTTCCGACCACAAGACATTCGGACAGTGCTGAATGGTCCCTTCAGAGAACTAAAACATGACTGC
  AACAGAGGACTGCCTGTCGTGGACAATGATGTGCCCCAGCCCAGACCTGGAGAGTGCATCACCAACAACA
  TGAAGCTCCGGCACTTTGGCTCATCTCTCTCCCTGCCTGACCGCGTACTCACCTTCATCCGGGACCACCC
  ACTCATGGACAGGCCAGTGTTTCCAGCTGATGGCCACCCCCTGCTGGTCACTACAGATACAGCCTATCTC
  AGAGTCGTGGCCCACAGGGTGACCAGCCTCTCAGGGAAAGAGTATGATGTGCTCTACCTGGGGACAGAGG
  ATGGACACCTCCACCGAGCAGTGCGGATCGGAGCTCAGCTCAGCGTTCTTGAAGATCTGGCCTTATTCCC
  AGAGCCACAGCCAGTTGAGAACATGAAATTGTACCACAGCTGGCTCCTGGTTGGCTCCCGTACTGAGGTG
  ACACAAGTGAATACAACCAACTGTGGCCGTCTCCAGAGCTGCTCAGAGTGCATCCTGGCCCAGGACCCAG
  TCTGTGCCTGGAGCTTCCGGCTGGATGAGTGTGTGGCCCATGCCGGGGAGCACCGAGGGTTGGTCCAAGA
  CATAGAGTCAGCAGATGTCTCCTCTTTGTGTCCTAAAGAGCCTGGAGAACGTCCAGTAGTGTTTGAAGTT
  CCCGTGGCTACAGCTGCGCATGTGGTCTTGCCATGTTCTCCAAGCTCAGCATGGGCATCCTGTGTGTGGC
  ACCAGCCCAGTGGAGTGACTGCACTCACCCCCCGGCGGGATGGACTGGAGGTGGTGGTGACCCCAGGGGC
  CATGGGCGCTTATGCCTGTGAATGTCAGGAGGGTGGGGCAGCCCATGTGGTAGCAGCTTACAGCTTGGTA
  TGGGGCAGCCAGCGAGATGCTCCGAGCCGGGCCCACACAGTGGGGGCGGGACTGGCTGGCTTCTTCTTGG
  GGATTCTCGCAGCATCCCTGACTCTCATTCTGATTGGTCGGCGTCAGCAGCGACGGCGACAGAGGGAACT
  TCTGGCTAGAGACAAGGTGGGCCTGGACCTGGGGGCTCCACCTTCTGGGACCACAAGCTACAGCCAAGAC
  CCTCCCTCCCCCTCTCCTGAAGATGAGCGGTTGCCGCTGGCCCTGGCCAAGAGGGGCAGTGGCTTTGGTG
  GATTCTCACCACCCTTCCTGCTTGATCCTTGCCCAAGCCCAGCCCACATTCGGCTAACTGGGGCTCCTCT
  AGCCACATGTGATGAAACATCCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233014 representing NM_001271661
  Red=Cloning site Green=Tags(s)
  
  MPASAARPRPGPGQPTASPFPLLLLAVLSGPVSGRVPRSVPRTSLPISEADSCLTRFAVPHTYNYSVLLV
  DPASHTLYVGARDTIFALSLPFSGERPRRIDWMVPEAHRQNCRKKGKKEGDLGGRKTLQQRWTTFLKADL
  LCPGPEHGRASSVLQDVAVLRPELGAGTPIFYGIFSSQWEGATISAVCAFRPQDIRTVLNGPFRELKHDC
  NRGLPVVDNDVPQPRPGECITNNMKLRHFGSSLSLPDRVLTFIRDHPLMDRPVFPADGHPLLVTTDTAYL
  RVVAHRVTSLSGKEYDVLYLGTEDGHLHRAVRIGAQLSVLEDLALFPEPQPVENMKLYHSWLLVGSRTEV
  TQVNTTNCGRLQSCSECILAQDPVCAWSFRLDECVAHAGEHRGLVQDIESADVSSLCPKEPGERPVVFEV
  PVATAAHVVLPCSPSSAWASCVWHQPSGVTALTPRRDGLEVVVTPGAMGAYACECQEGGAAHVVAAYSLV
  WGSQRDAPSRAHTVGAGLAGFFLGILAASLTLILIGRRQQRRRQRELLARDKVGLDLGAPPSGTTSYSQD
  PPSPSPEDERLPLALAKRGSGFGGFSPPFLLDPCPSPAHIRLTGAPLATCDETSI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271661
• ORF Size: 1845 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271661.1, NP_001258590.1
• RefSeq Size: 5657
• RefSeq ORF: 1848
• Locus ID: 10505
• Protein Families: Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]