CUX1 (NM_001202545) Human Tagged ORF Clone

CAT#: RG233030

CUX1 (GFP-tagged) - Homo sapiens cut-like homeobox 1 (CUX1), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CUX1
• Synonyms: CASP; CDP; CDP/Cut; CDP1; Clox; COY1; CUTL1; CUX; Cux/CDP; GDDI; GOLIM6; Nbla10317; p75; p100; p110; p200
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233030 representing NM_001202545
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCAATGTGGGATCGATGTTTCAATATTGGAAGCGCTTTGATTTACAGCAGCTGCAGAGAGAAC
  TCGATGCCACCGCAACGGTATTGGCGAACCGGCAGGATGAAAGTGAGCAGTCCAGAAAGCGGCTTATCGA
  ACAGAGCCGGGAGTTCAAGAAGAACACTCCAGAGGATTTGCGCAAGCAGGTAGCGCCGCTGCTGAAGAGT
  TTCCAAGGAGAGATTGATGCACTGAGTAAAAGAAGCAAGGAAGCTGAAGCAGCTTTCTTGAATGTCTACA
  AAAGATTGATTGACGTCCCAGAGGTTACGATAAAAGCACTTAAAGAGAAAATCCGAGAATATGAACAGAC
  ACTGAAGAACCAAGCCGAAACCATAGCTCTTGAGAAGGAACAGAAGTTACAGAATGACTTTGCAGAAAAG
  GAGAGAAAGCTGCAGGAGACACAGATGTCCACCACCTCAAAGCTGGAGGAAGCTGAGCATAAGGTTCAGA
  GCCTACAAACAGCCCTGGAAAAAACTCGAACAGAATTATTTGACCTGAAAACCAAATACGATGAAGAAAC
  TACTGCAAAGGCCGACGAGATTGAAATGATCATGACGGACCTTGAAAGGGCAAACCAGAGGGCAGAGGTG
  GCTCAGAGAGAGGCGGAGACCTTAAGGGAACAGCTCTCATCGGCCAATCACTCCCTCCAGCTGGCCTCAC
  AGATCCAGAAGGCACCAGACGTGGAGCAGGCCATAGAGGTGCTGACCCGCTCCAGCCTAGAAGTTGAGTT
  GGCCGCCAAGGAGCGGGAGATCGCACAGCTGGTGGAGGACGTGCAGAGACTCCAGGCCAGCCTCACCAAG
  CTGCGGGAGAATTCGGCCAGCCAGATCTCACAGCTTGAGCAGCAGCTGAGCGCCAAAAACAGCACACTCA
  AACAACTGGAAGAAAAACTCAAAGGCCAGGCTGACTATGAAGAGGTGAAGAAAGAGCTGAACATTCTGAA
  GTCCATGGAGTTTGCACCGTCCGAGGGCGCTGGGACACAGGATGCGGCCAAGCCCCTGGAGGTGCTGTTG
  CTGGAGAAGAACCGCTCGCTGCAGTCCGAGAACGCCGCGCTGCGCATCTCCAACAGCGACCTGAGCGGAC
  GCTGTGCGGAGCTGCAAGTCCGTATCACTGAGGCTGTGGCCACAGCCACTGAGCAGAGAGAGCTGATCGC
  CCGCCTGGAGCAGGACCTGAGCATCATTCAGTCCATCCAGCGGCCCGATGCCGAGGGTGCCGCTGAGCAC
  CGCCTGGAGAAGATCCCAGAGCCCATCAAAGAGGCCACTGCCCTATTCTACGGACCTGCAGCACCAGCCA
  GCGGTGCCCTCCCAGAGGGCCAGGTGGATTCACTGCTTTCCATCATCTCCAGCCAGAGGGAGCGCTTCCG
  TGCCCGGAACCAGGAGCTTGAGGCCGAGAACCGCCTGGCCCAGCACACCCTCCAGGCCCTGCAGAGTGAG
  CTGGACAGCCTGCGCGCCGACAACATCAAGCTCTTTGAGAAGATCAAGTTCCTGCAGAGCTACCCTGGCC
  GGGGCAGCGGCAGTGATGACACGGAGCTGCGGTACTCGTCCCAGTACGAGGAGCGCCTGGACCCCTTCTC
  CTCCTTCAGCAAGCGGGAGCGGCAGAGGAAGTACCTGAGCTTGAGTCCCTGGGACAAGGCCACCCTCAGC
  ATGGGGCGTCTGGTTCTCTCCAACAAGATGGCGCGCACCATCGGCTTCTTCTACACACTGTTCCTGCACT
  GCCTGGTCTTCCTGGTGCTCTACAAGCTGGCATGGAGCGAGAGCATGGAGAGGGACTGTGCCACCTTCTG
  CGCCAAGAAGTTCGCTGACCACCTGCACAAGTTCCACGAGAATGACAACGGGGCTGCGGCTGGTGACTTG
  TGGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233030 representing NM_001202545
  Red=Cloning site Green=Tags(s)
  
  MAANVGSMFQYWKRFDLQQLQRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKS
  FQGEIDALSKRSKEAEAAFLNVYKRLIDVPEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEK
  ERKLQETQMSTTSKLEEAEHKVQSLQTALEKTRTELFDLKTKYDEETTAKADEIEMIMTDLERANQRAEV
  AQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEVELAAKEREIAQLVEDVQRLQASLTK
  LRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNILKSMEFAPSEGAGTQDAAKPLEVLL
  LEKNRSLQSENAALRISNSDLSGRCAELQVRITEAVATATEQRELIARLEQDLSIIQSIQRPDAEGAAEH
  RLEKIPEPIKEATALFYGPAAPASGALPEGQVDSLLSIISSQRERFRARNQELEAENRLAQHTLQALQSE
  LDSLRADNIKLFEKIKFLQSYPGRGSGSDDTELRYSSQYEERLDPFSSFSKRERQRKYLSLSPWDKATLS
  MGRLVLSNKMARTIGFFYTLFLHCLVFLVLYKLAWSESMERDCATFCAKKFADHLHKFHENDNGAAAGDL
  WQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001202545
• ORF Size: 1896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001202545.2, NP_001189474.1
• RefSeq Size: 2901 bp
• RefSeq ORF: 1899 bp
• Locus ID: 1523
• Cytogenetics: 7q22.1
• Protein Families: Transcription Factors, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011]'