Growth hormone receptor (GHR) (NM_001242399) Human Tagged ORF Clone

CAT#: RG233054

GHR (GFP-tagged) - Homo sapiens growth hormone receptor (GHR), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GHR
• Synonyms: GHBP; GHIP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233054 representing NM_001242399
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTGGGGTCCTACAGGTATGGATCTCTGGCAGCTGCTGTTGACCTTGGCACTGGCAGGATCAAGTG
  ATGCTTTTTCTGGAAGTGAGGCCACAGCAGCTATCCTTAGCAGAGCACCCTGGAGTCTGCAAAGTGTTAA
  TCCAGGCCTAAAGACAAATTCTTCTAAGGAGCCTAAATTCACCAAGTGCCGTTCACCTGAGCGAGAGACT
  TTTTCATGCCACTGGACAGATGAGGTTCATCATGGTACAAAGAACCTAGGACCCATACAGCTGTTCTATA
  CCAGAAGGAACACTCAAGAATGGACTCAAGAATGGAAAGAATGCCCTGATTATGTTTCTGCTGGGGAAAA
  CAGCTGTTACTTTAATTCATCGTTTACCTCCATCTGGATACCTTATTGTATCAAGCTAACTAGCAATGGT
  GGTACAGTGGATGAAAAGTGTTTCTCTGTTGATGAAATAGTGCAACCAGATCCACCCATTGCCCTCAACT
  GGACTTTACTGAACGTCAGTTTAACTGGGATTCATGCAGATATCCAAGTGAGATGGGAAGCACCACGCAA
  TGCAGATATTCAGAAAGGATGGATGGTTCTGGAGTATGAACTTCAATACAAAGAAGTAAATGAAACTAAA
  TGGAAAATGATGGACCCTATATTGACAACATCAGTTCCAGTGTACTCATTGAAAGTGGATAAGGAATATG
  AAGTGCGTGTGAGATCCAAACAACGAAACTCTGGAAATTATGGCGAGTTCAGTGAGGTGCTCTATGTAAC
  ACTTCCTCAGATGAGCCAATTTACATGTGAAGAAGATTTCTACTTTCCATGGCTCTTAATTATTATCTTT
  GGAATATTTGGGCTAACAGTGATGCTATTTGTATTCTTATTTTCTAAACAGCAAAGGATTAAAATGCTGA
  TTCTGCCCCCAGTTCCAGTTCCAAAGATTAAAGGAATCGATCCAGATCTCCTCAAGGAAGGAAAATTAGA
  GGAGGTGAACACAATCTTAGCCATTCATGATAGCTATAAACCCGAATTCCACAGTGATGACTCTTGGGTT
  GAATTTATTGAGCTAGATATTGATGAGCCAGATGAAAAGACTGAGGAATCAGACACAGACAGACTTCTAA
  GCAGTGACCATGAGAAATCACATAGTAACCTAGGGGTGAAGGATGGCGACTCTGGACGTACCAGCTGTTG
  TGAACCTGACATTCTGGAGACTGATTTCAATGCCAATGACATACATGAGGGTACCTCAGAGGTTGCTCAG
  CCACAGAGGTTAAAAGGGGAAGCAGATCTCTTATGCCTTGACCAGAAGAATCAAAATAACTCACCTTATC
  ATGATGCTTGCCCTGCTACTCAGCAGCCCAGTGTTATCCAAGCAGAGAAAAACAAACCACAACCACTTCC
  TACTGAAGGAGCTGAGTCAACTCACCAAGCTGCCCATATTCAGCTAAGCAATCCAAGTTCACTGTCAAAC
  ATCGACTTTTATGCCCAGGTGAGCGACATTACACCAGCAGGTAGTGTGGTCCTTTCCCCGGGCCAAAAGA
  ATAAGGCAGGGATGTCCCAATGTGACATGCACCCGGAAATGGTCTCACTCTGCCAAGAAAACTTCCTTAT
  GGACAATGCCTACTTCTGTGAGGCAGATGCCAAAAAGTGCATCCCTGTGGCTCCTCACATCAAGGTTGAA
  TCACACATACAGCCAAGCTTAAACCAAGAGGACATTTACATCACCACAGAAAGCCTTACCACTGCTGCTG
  GGAGGCCTGGGACAGGAGAACATGTTCCAGGTTCTGAGATGCCTGTCCCAGACTATACCTCCATTCATAT
  AGTACAGTCCCCACAGGGCCTCATACTCAATGCGACTGCCTTGCCCTTGCCTGACAAAGAGTTTCTCTCA
  TCATGTGGCTATGTGAGCACAGACCAACTGAACAAAATCATGCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233054 representing NM_001242399
  Red=Cloning site Green=Tags(s)
  
  MNWGPTGMDLWQLLLTLALAGSSDAFSGSEATAAILSRAPWSLQSVNPGLKTNSSKEPKFTKCRSPERET
  FSCHWTDEVHHGTKNLGPIQLFYTRRNTQEWTQEWKECPDYVSAGENSCYFNSSFTSIWIPYCIKLTSNG
  GTVDEKCFSVDEIVQPDPPIALNWTLLNVSLTGIHADIQVRWEAPRNADIQKGWMVLEYELQYKEVNETK
  WKMMDPILTTSVPVYSLKVDKEYEVRVRSKQRNSGNYGEFSEVLYVTLPQMSQFTCEEDFYFPWLLIIIF
  GIFGLTVMLFVFLFSKQQRIKMLILPPVPVPKIKGIDPDLLKEGKLEEVNTILAIHDSYKPEFHSDDSWV
  EFIELDIDEPDEKTEESDTDRLLSSDHEKSHSNLGVKDGDSGRTSCCEPDILETDFNANDIHEGTSEVAQ
  PQRLKGEADLLCLDQKNQNNSPYHDACPATQQPSVIQAEKNKPQPLPTEGAESTHQAAHIQLSNPSSLSN
  IDFYAQVSDITPAGSVVLSPGQKNKAGMSQCDMHPEMVSLCQENFLMDNAYFCEADAKKCIPVAPHIKVE
  SHIQPSLNQEDIYITTESLTTAAGRPGTGEHVPGSEMPVPDYTSIHIVQSPQGLILNATALPLPDKEFLS
  SCGYVSTDQLNKIMP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242399
• ORF Size: 1935 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242399.2, NP_001229328.1
• RefSeq Size: 4529 bp
• RefSeq ORF: 1938 bp
• Locus ID: 2690
• Cytogenetics: 5p13.1-p12
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]'