NDUFS1 (NM_001199983) Human Tagged ORF Clone

CAT#: RG233068

NDUFS1 (GFP-tagged) - Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) (NDUFS1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NDUFS1
• Synonyms: CI-75k; CI-75Kd; MC1DN5; PRO1304
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233068 representing NM_001199983
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATCCCTCGATTCTGTTATCATGAAAGGTTGTCTGTTGCTGGAAACTGCAGGATGTGCCTTGTTG
  AAATTGAGAAAGCCCCTAAGGTTGTAGCTGCTTGTGCCATGCCAGTAATGAAGGGTTGGAATATCCTAAC
  AAACTCAGAAAAATCCAAAAAAGCCAGGGAAGGTGTGATGGAGTTCTTATTAGCAAATCACCCATTGGAC
  TGTCCTATTTGTGACCAGGGAGGTGAATGTGATCTGCAGGACCAGTCCATGATGTTTGGAAATGATAGGA
  GCCGATTTTTAGAGGGGAAGCGTGCTGTGGAAGACAAGAACATTGGGCCATTGGTAAAGACCATCATGAC
  AAGATGTATACAGTGTACTCGCTGCATCAGGTTTGCAAGTGAGATTGCAGGAGTAGATGATTTGGGAACA
  ACAGGCAGAGGAAATGATATGCAAGTTGGCACATACATTGAAAAGATGTTCATGTCTGAACTGTCTGGGA
  ATATCATTGATATCTGCCCTGTAGGTGCCCTAACCTCTAAGCCCTATGCCTTTACTGCCCGGCCTTGGGA
  AACAAGAAAGACAGAATCCATTGATGTAATGGATGCGGTTGGAAGTAATATTGTGGTTAGCACAAGAACT
  GGAGAAGTGATGAGGATTTTGCCACGTATGCATGAGGACATCAATGAAGAGTGGATCTCTGATAAAACCA
  GATTTGCCTATGATGGGCTAAAACGTCAAAGACTTACCGAGCCAATGGTCAGAAATGAAAAAGGGCTTTT
  AACCTATACTTCTTGGGAGGATGCGCTCTCTCGCGTAGCTGGAATGTTGCAGAGTTTTCAAGGCAAAGAT
  GTGGCAGCAATTGCAGGTGGCTTGGTGGATGCTGAAGCCCTGGTAGCTCTCAAAGATTTGCTTAATAGAG
  TGGACTCTGACACCTTATGCACTGAAGAGGTCTTCCCCACTGCAGGAGCTGGCACAGATTTGCGTTCCAA
  TTATCTTCTTAATACTACAATTGCTGGTGTGGAAGAGGCAGATGTTGTTCTTCTGGTTGGTACAAACCCA
  CGTTTTGAGGCACCACTGTTTAATGCTAGAATTCGAAAGAGCTGGCTGCATAATGACTTAAAAGTGGCCC
  TTATAGGCAGTCCAGTGGACCTCACTTACACATATGACCACCTGGGAGACTCCCCCAAAATTCTTCAAGA
  CATTGCTTCGGGAAGCCATCCATTTAGCCAGGTCCTAAAGGAAGCTAAAAAACCAATGGTGGTTTTAGGC
  AGTTCTGCACTCCAAAGAAATGATGGAGCAGCAATTCTTGCAGCTGTTTCTAGCATTGCACAAAAGATTC
  GGATGACTAGTGGTGTTACTGGTGATTGGAAAGTTATGAATATCCTTCATAGGATTGCAAGTCAAGTAGC
  TGCTTTGGACCTTGGCTATAAGCCTGGGGTGGAAGCAATTCGGAAGAACCCTCCCAAGGTGCTGTTTCTC
  CTGGGAGCAGATGGAGGTTGTATCACACGACAGGATTTGCCAAAGGATTGTTTCATTATTTATCAAGGAC
  ATCATGGTGATGTTGGGGCTCCCATAGCTGATGTTATTCTCCCAGGAGCTGCTTACACAGAGAAGTCTGC
  TACATATGTCAACACTGAGGGTAGAGCTCAGCAGACTAAGGTAGCAGTGACACCTCCTGGCTTGGCAAGA
  GAAGACTGGAAAATTATAAGAGCACTCTCTGAGATTGCTGGAATGACTCTTCCATATGATACTCTGGATC
  AAGTAAGGAACAGATTGGAAGAAGTCTCTCCTAATCTTGTTCGATATGATGATATTGAAGGGGCTAATTA
  CTTCCAGCAAGCAAATGAGCTCTCAAAGCTAGTGAACCAGCAGCTTCTTGCTGACCCACTTGTTCCACCT
  CAGCTAACTATAAAAGACTTCTACATGACAGATTCAATTAGCAGAGCCTCACAGACAATGGCCAAATGTG
  TCAAAGCTGTCACAGAGGGTGCCCAGGCAGTAGAGGAACCATCCATATGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233068 representing NM_001199983
  Red=Cloning site Green=Tags(s)
  
  MQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLD
  CPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGT
  TGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRT
  GEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQSFQGKD
  VAAIAGGLVDAEALVALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNP
  RFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYTYDHLGDSPKILQDIASGSHPFSQVLKEAKKPMVVLG
  SSALQRNDGAAILAAVSSIAQKIRMTSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKVLFL
  LGADGGCITRQDLPKDCFIIYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAR
  EDWKIIRALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDIEGANYFQQANELSKLVNQQLLADPLVPP
  QLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199983
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199983.1, NP_001186912.1
• RefSeq Size: 3381 bp
• RefSeq ORF: 2013 bp
• Locus ID: 4719
• Cytogenetics: 2q33.3
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]'