USP10 (NM_001272075) Human Tagged ORF Clone

CAT#: RG233142

USP10 (GFP-tagged) - Homo sapiens ubiquitin specific peptidase 10 (USP10), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: USP10
• Synonyms: UBPO
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233142 representing NM_001272075
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTGGTTGCCCTCTCCTGGAATAGGGCAGTATATTTTTGGAGATTTTAGCCCTGATGAATTCAATC
  AATTCTTTGTGACTCCTCGATCTTCAGTTGAGCTTCCTCCATACAGTGGAACAGTTCTGTGTGGCACACA
  GGCTGTGGATAAACTACCTGATGGACAAGAATATCAGAGAATTGAGTTTGGTGTCGATGAAGTCATTGAA
  CCCAGTGACACTTTGCCGAGAACCCCCAGCTACAGTATTTCAAGCACACTGAACCCTCAGGCCCCTGAAT
  TTATTCTCGGTTGTACAGCTTCCAAAATAACCCCTGATGGTATCACTAAAGAAGCAAGCTATGGCTCCAT
  CGACTGCCAGTACCCAGGCTCTGCCCTCGCTTTGGATGGAAGTTCTAATGTGGAGGCGGAAGTTTTGGAA
  AATGATGGTGTCTCAGGTGGTCTTGGACAAAGGGAGCGTAAAAAGAAGAAAAAGCGGCCACCTGGATATT
  ACAGCTATTTGAAAGATGGTGGCGATGATAGTATCTCCACAGAAGCCCTGGTCAATGGCCATGCCAATTC
  AGCAGTCCCGAACAGTGTCAGTGCAGAGGATGCAGAATTTATGGGTGACATGCCCCCGTCAGTTACGCCC
  AGGACTTGTAACAGCCCCCAGAACTCCACAGACTCTGTCAGTGACATTGTGCCTGACAGTCCTTTCCCCG
  GAGCACTCGGCAGTGACACCAGGACTGCAGGGCAGCCAGAGGGGGGCCCCGGGGCTGATTTTGGTCAGTC
  CTGCTTCCCTGCAGAGGCTGGCAGAGACACCCTGTCAAGGACAGCTGGGGCTCAGCCCTGCGTTGGTACC
  GATACTACTGAAAACCTTGGAGTTGCTAATGGACAAATACTTGAATCCTCGGGTGAGGGCACAGCTACCA
  ACGGGGTGGAGTTGCACACCACGGAAAGCATAGACTTGGACCCAACCAAACCCGAGAGTGCATCACCTCC
  TGCTGACGGCACGGGCTCTGCATCAGGCACCCTTCCTGTCAGCCAGCCCAAGTCCTGGGCCAGCCTCTTT
  CATGATTCTAAGCCCTCTTCCTCCTCGCCGGTGGCCTATGTGGAAACTAAGTATTCCCCTCCCGCCATAT
  CTCCCCTGGTTTCTGAAAAGCAGGTTGAAGTCAAAGAAGGGCTTGTTCCGGTTTCAGAGGATCCTGTAGC
  CATAAAGATTGCAGAGTTGCTGGAGAATGTAACCCTAATCCATAAACCAGTGTCGTTGCAACCCCGTGGG
  CTGATCAATAAAGGGAACTGGTGCTACATTAATGCTACACTGCAGGCATTGGTTGCTTGCCCGCCGATGT
  ACCACCTGATGAAGTTCATTCCTCTGTATTCCAAAGTGCAAAGGCCTTGTACGTCAACACCCATGATAGA
  CAGCTTTGTTCGGCTAATGAATGAGTTCACTAATATGCCAGTACCTCCAAAACCCCGACAAGCTCTTGGA
  GATAAAATCGTGAGGGATATTCGCCCTGGAGCTGCCTTTGAGCCCACATATATTTACAGACTCCTGACAG
  TTAACAAGTCAAGCCTGTCTGAAAAGGGTCGACAAGAAGATGCTGAGGAATACTTAGGCTTCATTCTAAA
  TGGACTTCATGAGGAAATGTTGAACCTAAAGAAGCTTCTCTCACCAAGTAATGAAAAACTTACGATTTCC
  AACGGCCCCAAAAACCACTCGGTCAATGAAGAAGAGCAGGAAGAACAAGGTGAAGGAAGCGAGGATGAAT
  GGGAACAAGTGGGCCCCCGGAACAAGACTTCCGTCACCCGCCAGGCGGATTTTGTTCAGACTCCAATCAC
  CGGCATTTTTGGTGGACACATCAGGTCTGTGGTTTACCAGCAGAGTTCAAAAGAATCTGCCACTTTGCAG
  CCATTTTTCACGTTGCAGTTGGATATCCAGTCAGACAAGATACGCACAGTCCAGGATGCACTGGAGAGCT
  TGGTGGCAAGAGAATCTGTCCAAGGTTATACCACAAAAACCAAACAAGAGGTTGAGATAAGTCGAAGAGT
  GACTCTGGAAAAACTCCCTCCTGTCCTCGTGCTGCACCTGAAACGATTCGTTTATGAGAAGACTGGTGGG
  TGCCAGAAGCTTATCAAAAATATTGAATATCCTGTGGACTTGGAAATTAGTAAAGAACTGCTTTCTCCAG
  GGGTTAAAAATAAGAATTTTAAATGCCACCGAACCTATCGGCTCTTTGCAGTGGTCTACCATCACGGCAA
  CAGTGCGACGGGCGGCCATTACACTACAGACGTCTTCCAGATCGGTCTGAATGGCTGGCTGCGCATCGAT
  GACCAGACAGTCAAGGTGATCAACCAGTACCAGGTGGTGAAACCAACTGCTGAACGCACAGCCTACCTCC
  TGTATTACCGCCGAGTGGACCTGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233142 representing NM_001272075
  Red=Cloning site Green=Tags(s)
  
  MPWLPSPGIGQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDGQEYQRIEFGVDEVIE
  PSDTLPRTPSYSISSTLNPQAPEFILGCTASKITPDGITKEASYGSIDCQYPGSALALDGSSNVEAEVLE
  NDGVSGGLGQRERKKKKKRPPGYYSYLKDGGDDSISTEALVNGHANSAVPNSVSAEDAEFMGDMPPSVTP
  RTCNSPQNSTDSVSDIVPDSPFPGALGSDTRTAGQPEGGPGADFGQSCFPAEAGRDTLSRTAGAQPCVGT
  DTTENLGVANGQILESSGEGTATNGVELHTTESIDLDPTKPESASPPADGTGSASGTLPVSQPKSWASLF
  HDSKPSSSSPVAYVETKYSPPAISPLVSEKQVEVKEGLVPVSEDPVAIKIAELLENVTLIHKPVSLQPRG
  LINKGNWCYINATLQALVACPPMYHLMKFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALG
  DKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTIS
  NGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQ
  PFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGG
  CQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRID
  DQTVKVINQYQVVKPTAERTAYLLYYRRVDLL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001272075
• ORF Size: 2406 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001272075.1, NP_001259004.1
• RefSeq Size: 3520
• RefSeq ORF: 2409
• Locus ID: 9100
• Protein Families: Druggable Genome, Protease
• Gene Summary: Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2013]