PSD93 (DLG2) (NM_001206769) Human Tagged ORF Clone

CAT#: RG233167

DLG2 (GFP-tagged) - Homo sapiens discs, large homolog 2 (Drosophila) (DLG2), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DLG2
• Synonyms: chapsyn-110; PPP1R58; PSD-93; PSD93
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233167 representing NM_001206769
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCATACCTCACCAAGCAACACAGCTGCAGCCGGGGGTCCGATGGGATGGATGCCGTCAGGAGTG
  CGCCCACCCTGATCAGGGATGCCCACTGTGCTTGTGGCTGGCAGAGGAACTGTCAGGGGCTCGGGTACAG
  TTCTCAGACCATGCCCTCCTCAGGACCAGGGGGCCCAGCTTCAAACAGGACCGGGGGGTCCAGCTTCAAC
  AGGACCTTGTGGGACAGTGTGCGGAAAAGCCCCCACAAGACGAGCACCAAGGGCAAAGGGACTTGTGGGG
  AGCATTGTACCTGCCCACATGGTTGGTTCAGCCCAGCACAGGCCAGTCCTGCTCCTATAATTGTCAACAC
  AGATACTTTGGACACAATTCCTTATGTCAATGGGACAGAAATTGAATATGAATTTGAAGAAATTACACTG
  GAGAGGGGGAATTCTGGCCTGGGATTCAGTATTGCTGGGGGGACAGATAATCCCCACATTGGAGATGACC
  CTGGCATATTTATTACGAAGATTATACCAGGAGGTGCTGCAGCAGAGGATGGCAGACTCAGGGTCAATGA
  TTGTATCTTGCGGGTGAATGAGGTTGATGTGTCAGAGGTTTCCCACAGTAAAGCGGTGGAAGCCCTGAAG
  GAAGCAGGGTCTATCGTTCGGCTGTATGTGCGTAGAAGACGACCTATTTTGGAGACCGTTGTGGAAATCA
  AACTGTTCAAAGGCCCTAAAGGTTTAGGCTTCAGTATTGCAGGAGGTGTGGGGAACCAACACATTCCTGG
  AGACAACAGCATTTATGTAACTAAAATTATAGATGGAGGAGCTGCACAAAAAGATGGAAGGTTGCAAGTA
  GGAGATAGACTACTAATGGTAAACAACTACAGTTTAGAAGAAGTAACACACGAAGAGGCAGTAGCAATAT
  TAAAGAACACATCAGAGGTAGTTTATTTAAAAGTTGGCAAACCCACTACCATTTATATGACTGATCCTTA
  TGGTCCACCTGATATTACTCACTCTTATTCTCCACCAATGGAAAACCATCTACTCTCTGGCAACAATGGC
  ACTTTAGAATATAAAACCTCCCTGCCACCCATCTCTCCAGGAAGGTACTCACCAATTCCAAAGCACATGC
  TTGTTGACGACGACTACACCAGGCCTCCGGAACCTGTTTACAGCACTGTGAACAAACTATGTGATAAGCC
  TGCTTCTCCCAGGCACTATTCCCCTGTTGAGTGTGACAAAAGCTTCCTCCTCTCAGCTCCCTATTCCCAC
  TACCACCTAGGCCTGCTACCTGACTCTGAGATGACCAGTCATTCCCAACATAGCACCGCAACTCGTCAGC
  CTTCAATGACTCTCCAACGGGCCGTCTCCCTGGAAGGAGAGCCTCGCAAGGTAGTCCTGCACAAAGGCTC
  CACTGGCCTGGGCTTCAACATTGTCGGTGGGGAAGATGGAGAAGGTATTTTTGTGTCCTTCATTCTGGCT
  GGTGGACCAGCAGACCTAAGTGGGGAGCTCCAGAGAGGAGACCAGATCCTATCGGTGAATGGCATTGACC
  TCCGTGGTGCATCCCACGAGCAGGCAGCTGCTGCACTAAAGGGGGCTGGACAGACAGTGACGATTATAGC
  ACAATATCAACCTGAAGATTACGCTCGATTTGAGGCCAAAATCCATGACCTACGAGAGCAGATGATGAAC
  CACAGCATGAGCTCCGGGTCCGGATCCCTGCGAACCAATCAGAAACGCTCCCTCTACGTCAGAGCCATGT
  TCGACTACGACAAGAGCAAGGACAGTGGGCTGCCAAGTCAAGGACTTAGTTTTAAATATGGAGATATTCT
  CCACGTTATCAATGCCTCTGATGATGAGTGGTGGCAAGCCAGGAGAGTCATGCTGGAGGGAGACAGTGAG
  GAGATGGGGGTCATCCCCAGCAAAAGGAGGGTGGAAAGAAAGGAACGTGCCCGATTGAAGACAGTGAAGT
  TTAATGCCAAACCTGGAGTGATTGATTCGAAAGGGTCATTCAATGACAAGCGTAAAAAGAGCTTCATCTT
  TTCACGAAAATTCCCATTCTACAAGAACAAGGAGCAGAGTGAGCAGGAAACCAGTGATCCTGAACGAGGA
  CAAGAAGACCTCATTCTTTCCTATGAGCCTGTTACAAGGCAGGAAATAAACTACACCCGGCCGGTGATTA
  TCCTGGGGCCCATGAAGGATCGGATCAATGACGACTTGATATCTGAATTCCCTGATAAATTTGGCTCCTG
  TGTGCCTCATACTACGAGGCCAAAGCGAGACTACGAGGTGGATGGCAGAGACTATCACTTTGTCATTTCC
  AGAGAACAAATGGAGAAAGATATCCAAGAGCACAAGTTTATAGAAGCCGGCCAGTACAATGACAATTTAT
  ATGGAACCAGTGTGCAGTCTGTGAGATTTGTAGCAGAAAGAGGCAAACACTGTATACTTGATGTATCAGG
  AAATGCTATCAAGCGGTTACAAGTTGCCCAGCTCTATCCCATTGCCATCTTCATAAAACCCAGGTCTCTG
  GAACCTCTTATGGAGATGAATAAGCGTCTAACAGAGGAACAAGCCAAGAAAACCTATGATCGAGCAATTA
  AGCTAGAACAAGAATTTGGAGAATATTTTACAGCTATTGTCCAAGGAGATACTTTAGAAGATATATATAA
  CCAATGCAAGCTTGTTATTGAAGAGCAATCTGGGCCTTTCATCTGGATTCCCTCAAAGGAAAAGTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233167 representing NM_001206769
  Red=Cloning site Green=Tags(s)
  
  MNAYLTKQHSCSRGSDGMDAVRSAPTLIRDAHCACGWQRNCQGLGYSSQTMPSSGPGGPASNRTGGSSFN
  RTLWDSVRKSPHKTSTKGKGTCGEHCTCPHGWFSPAQASPAPIIVNTDTLDTIPYVNGTEIEYEFEEITL
  ERGNSGLGFSIAGGTDNPHIGDDPGIFITKIIPGGAAAEDGRLRVNDCILRVNEVDVSEVSHSKAVEALK
  EAGSIVRLYVRRRRPILETVVEIKLFKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIDGGAAQKDGRLQV
  GDRLLMVNNYSLEEVTHEEAVAILKNTSEVVYLKVGKPTTIYMTDPYGPPDITHSYSPPMENHLLSGNNG
  TLEYKTSLPPISPGRYSPIPKHMLVDDDYTRPPEPVYSTVNKLCDKPASPRHYSPVECDKSFLLSAPYSH
  YHLGLLPDSEMTSHSQHSTATRQPSMTLQRAVSLEGEPRKVVLHKGSTGLGFNIVGGEDGEGIFVSFILA
  GGPADLSGELQRGDQILSVNGIDLRGASHEQAAAALKGAGQTVTIIAQYQPEDYARFEAKIHDLREQMMN
  HSMSSGSGSLRTNQKRSLYVRAMFDYDKSKDSGLPSQGLSFKYGDILHVINASDDEWWQARRVMLEGDSE
  EMGVIPSKRRVERKERARLKTVKFNAKPGVIDSKGSFNDKRKKSFIFSRKFPFYKNKEQSEQETSDPERG
  QEDLILSYEPVTRQEINYTRPVIILGPMKDRINDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVIS
  REQMEKDIQEHKFIEAGQYNDNLYGTSVQSVRFVAERGKHCILDVSGNAIKRLQVAQLYPIAIFIKPRSL
  EPLMEMNKRLTEEQAKKTYDRAIKLEQEFGEYFTAIVQGDTLEDIYNQCKLVIEEQSGPFIWIPSKEKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001206769
• ORF Size: 2727 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001206769.1, NP_001193698.1
• RefSeq Size: 7730 bp
• RefSeq ORF: 2730 bp
• Locus ID: 1740
• Cytogenetics: 11q14.1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. The encoded protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described, but their full-length nature is not known. [provided by RefSeq, Dec 2008]'