RBM15 (NM_001201545) Human Tagged ORF Clone

CAT#: RG233225

RBM15 (GFP-tagged) - Homo sapiens RNA binding motif protein 15 (RBM15), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RBM15
• Synonyms: OTT; OTT1; SPEN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233225 representing NM_001201545
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGACTGCGGGGCGGGACCCTGTGCCGCGGCGGAGTCCAAGATGGCGGCGTGCGGTTCCGCTGTGTG
  AAACGAGCGCGGGGCGGCGGGTTACTCAGCTCCGCGGAGACGACCTCCGACGACCCGCAACAATGAAGGG
  AAAAGAGCGCTCGCCAGTGAAGGCCAAACGCTCCCGTGGTGGTGAGGACTCGACTTCCCGCGGTGAGCGG
  AGCAAGAAGTTAGGGGGCTCTGGTGGCAGCAATGGGAGCAGCAGCGGAAAGACCGATAGCGGCGGTGGGT
  CGCGGCGGAGTCTCCACCTGGACAAGTCCAGCAGTCGAGGTGGCAGCCGCGAGTATGATACCGGTGGGGG
  CAGCTCCAGTAGCCGCTTGCATAGTTATAGCTCCCCGAGCACCAAAAATTCTTCGGGCGGGGGCGAGTCG
  CGCAGCAGCTCCCGGGGTGGAGGCGGGGAGTCACGTTCCTCTGGGGCCGCCTCCTCAGCTCCCGGCGGCG
  GGGACGGCGCGGAATACAAGACTCTGAAGATAAGCGAGTTGGGGTCCCAGCTTAGTGACGAAGCGGTGGA
  GGACGGCCTGTTTCATGAGTTCAAACGCTTCGGTGATGTAAGTGTGAAAATCAGTCATCTGTCGGGTTCT
  GGCAGCGGGGATGAGCGGGTAGCCTTTGTGAACTTCCGGCGGCCAGAGGACGCGCGGGCGGCCAAGCATG
  CCAGAGGCCGCCTGGTGCTCTATGACCGGCCTCTGAAGATAGAAGCTGTGTATGTGAGCCGGCGCCGCAG
  CCGCTCCCCTTTAGACAAAGATACTTATCCTCCATCAGCCAGTGTGGTCGGGGCCTCTGTAGGTGGTCAC
  CGGCACCCCCCTGGAGGTGGTGGAGGCCAGAGATCACTTTCCCCTGGTGGCGCTGCTTTGGGATACAGAG
  ACTACCGGCTGCAGCAGTTGGCTCTTGGCCGCCTGCCCCCTCCACCTCCGCCACCATTGCCTCGAGACCT
  GGAGAGAGAAAGAGACTACCCGTTCTATGAGAGAGTGCGCCCTGCATACAGTCTTGAGCCAAGGGTGGGA
  GCTGGAGCAGGTGCTGCTCCTTTCAGAGAAGTGGATGAGATTTCACCCGAGGATGATCAGCGAGCTAACC
  GGACGCTCTTCTTGGGCAACCTAGACATCACTGTAACGGAGAGTGATTTAAGAAGGGCGTTTGATCGCTT
  TGGAGTCATCACAGAAGTAGATATCAAGAGGCCTTCTCGCGGCCAGACTAGTACTTACGGCTTTCTCAAA
  TTTGAGAACTTAGATATGTCTCACCGGGCCAAATTAGCAATGTCTGGCAAAATTATAATTCGGAATCCTA
  TCAAAATTGGTTATGGTAAAGCTACACCCACCACCCGCCTCTGGGTGGGAGGCCTGGGACCTTGGGTTCC
  TCTTGCTGCCCTGGCACGAGAATTTGATCGATTTGGCACCATACGCACCATAGACTACCGAAAAGGTGAT
  AGTTGGGCATATATCCAGTATGAAAGCCTGGATGCAGCGCATGCTGCCTGGACCCATATGCGGGGCTTCC
  CACTTGGTGGCCCAGATCGACGCCTTAGAGTAGACTTTGCCGACACCGAACATCGTTACCAGCAGCAGTA
  TCTGCAGCCTCTGCCCTTGACTCATTATGAGCTGGTGACAGATGCTTTTGGACATCGGGCACCAGACCCT
  TTGAGGGGTGCTCGGGATAGGACACCACCCTTACTATACAGAGATCGTGATAGGGACCTTTATCCTGACT
  CTGATTGGGTGCCACCCCCACCCCCAGTCCGAGAACGCAGCACTCGGACTGCAGCTACTTCTGTGCCTGC
  TTACGAGCCACTGGATAGCCTAGATCGCAGGCGGGATGGTTGGTCCTTGGACCGGGACAGAGGTGATCGA
  GATCTGCCCAGCAGCAGAGACCAGCCTAGGAAGCGAAGGCTGCCTGAGGAGAGTGGAGGACGTCATCTGG
  ATAGGTCTCCTGAGAGTGACCGCCCACGAAAACGTCACTGCGCTCCTTCTCCTGACCGCAGTCCAGAATT
  GAGCAGTAGCCGGGATCGTTACAACAGCGACAATGATCGATCTTCCCGTCTTCTCTTGGAAAGGCCCTCT
  CCAATCAGAGACAGACGAGGTAGTTTGGAGAAGAGCCAGGGTGACAAGCGAGACCGTAAAAACTCTGCAT
  CAGCTGAACGAGATAGGAAGCACCGGACAACTGCTCCCACTGAGGGAAAAAGCCCTCTGAAAAAAGAAGA
  CCGCTCTGATGGGAGTGCACCTAGCACCAGCACTGCTTCCTCCAAGCTGAAGTCCCCGTCCCAGAAACAG
  GATGGGGGGACAGCCCCTGTGGCATCAGCCTCTCCCAAACTCTGTTTGGCCTGGCAGGGCATGCTTCTAC
  TGAAGAACAGCAACTTTCCTTCCAACATGCATCTGTTGCAGGGTGACCTCCAAGTGGCTAGTAGTCTTCT
  TGTGGAGGGTTCAACTGGAGGCAAAGTGGCCCAGCTCAAGATCACTCAGCGTCTCCGTTTGGACCAGCCC
  AAGTTGGATGAAGTAACTCGACGCATCAAAGTAGCAGGGCCCAATGGTTATGCCATTCTTTTGGCTGTGC
  CTGGAAGTTCTGACAGCCGGTCCTCCTCTTCCTCAGCTGCATCAGACACTGCCACTTCTACTCAGAGGCC
  ACTTAGGAACCTTGTGTCCTATTTAAAGCAAAAGCAGGCAGCCGGGGTGATCAGCCTCCCTGTGGGGGGC
  AACAAAGACAAGGAAAACACCGGGGTCCTTCATGCCTTCCCACCTTGTGAGTTCTCCCAGCAGTTCCTGG
  ATTCCCCTGCCAAGGCACTGGCCAAATCTGAAGAAGATTACCTGGTCATGATCATTGTCCGTGCAAAACT
  GGTTGAACAGCGGATGAAGATATGGAATTCAAAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233225 representing NM_001201545
  Red=Cloning site Green=Tags(s)
  
  MRTAGRDPVPRRSPRWRRAVPLCETSAGRRVTQLRGDDLRRPATMKGKERSPVKAKRSRGGEDSTSRGER
  SKKLGGSGGSNGSSSGKTDSGGGSRRSLHLDKSSSRGGSREYDTGGGSSSSRLHSYSSPSTKNSSGGGES
  RSSSRGGGGESRSSGAASSAPGGGDGAEYKTLKISELGSQLSDEAVEDGLFHEFKRFGDVSVKISHLSGS
  GSGDERVAFVNFRRPEDARAAKHARGRLVLYDRPLKIEAVYVSRRRSRSPLDKDTYPPSASVVGASVGGH
  RHPPGGGGGQRSLSPGGAALGYRDYRLQQLALGRLPPPPPPPLPRDLERERDYPFYERVRPAYSLEPRVG
  AGAGAAPFREVDEISPEDDQRANRTLFLGNLDITVTESDLRRAFDRFGVITEVDIKRPSRGQTSTYGFLK
  FENLDMSHRAKLAMSGKIIIRNPIKIGYGKATPTTRLWVGGLGPWVPLAALAREFDRFGTIRTIDYRKGD
  SWAYIQYESLDAAHAAWTHMRGFPLGGPDRRLRVDFADTEHRYQQQYLQPLPLTHYELVTDAFGHRAPDP
  LRGARDRTPPLLYRDRDRDLYPDSDWVPPPPPVRERSTRTAATSVPAYEPLDSLDRRRDGWSLDRDRGDR
  DLPSSRDQPRKRRLPEESGGRHLDRSPESDRPRKRHCAPSPDRSPELSSSRDRYNSDNDRSSRLLLERPS
  PIRDRRGSLEKSQGDKRDRKNSASAERDRKHRTTAPTEGKSPLKKEDRSDGSAPSTSTASSKLKSPSQKQ
  DGGTAPVASASPKLCLAWQGMLLLKNSNFPSNMHLLQGDLQVASSLLVEGSTGGKVAQLKITQRLRLDQP
  KLDEVTRRIKVAGPNGYAILLAVPGSSDSRSSSSSAASDTATSTQRPLRNLVSYLKQKQAAGVISLPVGG
  NKDKENTGVLHAFPPCEFSQQFLDSPAKALAKSEEDYLVMIIVRAKLVEQRMKIWNSKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001201545
• ORF Size: 2907 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001201545.1, NP_001188474.1
• RefSeq Size: 3338
• RefSeq ORF: 2910
• Locus ID: 64783
• Protein Families: Transcription Factors
• Gene Summary: Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]). [supplied by OMIM, Feb 2009]