PUS7L (NM_001271826) Human Tagged ORF Clone

CAT#: RG233952

PUS7L (GFP-tagged) - Homo sapiens pseudouridylate synthase 7 homolog (S. cerevisiae)-like (PUS7L), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PUS7L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233952 representing NM_001271826
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGAAGCGATTGGTTTTTTAGCTATCAAACTTGGTGTTATTCCTTCGGATTTTAGTTATGCAGGCC
  TTAAAGACAAGAAAGCCATCACCTATCAAGCAATGGTTGTTAGAAAAGTGACTCCAGAGAGGTTGAAAAA
  TATTGAAAAAGAAATTGAAAAGAAAAGAATGAATGTCTTTAATATTCGGTCTGTAGATGATTCCCTGAGA
  CTTGGTCAGCTCAAAGGAAATCACTTTGATATTGTCATTAGAAATTTAAAAAAACAAATAAATGATTCTG
  CAAACCTGAGGGAGAGAATTATGGAAGCAATAGAAAATGTTAAGAAAAAAGGCTTTGTGAATTACTATGG
  ACCACAGAGATTTGGGAAGGGAAGGAAAGTTCACACAGACCAAATTGGACTAGCTTTGCTGAAGAATGAA
  ATGATGAAAGCCATAAAATTGTTTCTTACACCAGAAGACTTGGATGATCCTGTAAATAGAGCAAAGAAGT
  ATTTTCTTCAAACTGAGGATGCTAAAGGCACACTTTCATTGATGCCTGAATTCAAAGTGCGTGAGAGAGC
  ATTGTTGGAGGCATTGCACCGCTTTGGCATGACCGAGGAAGGTTGTATCCAGGCATGGTTCTCTTTACCC
  CATTCCATGCGCATATTCTATGTTCACGCATATACCAGCAAAATTTGGAATGAGGCAGTATCTTACAGAC
  TTGAAACCTATGGAGCAAGAGTAGTGCAGGGTGATTTGGTCTGTTTGGATGAAGACATTGATGACGAGAA
  TTTCCCAAATAGTAAAATTCACCTGGTAACTGAAGAGGAGGGATCAGCTAATATGTATGCAATACATCAG
  GTGGTTCTTCCAGTACTTGGATACAATATTCAGTACCCGAAGAACAAAGTAGGGCAGTGGTACCATGACA
  TACTTAGCAGAGATGGACTACAGACATGTAGGTTTAAAGTACCTACTCTGAAACTGAATATACCAGGTTG
  CTATAGACAGATTTTGAAACATCCCTGTAATCTCTCATACCAACTAATGGAAGATCATGACATTGATGTC
  AAAACGAAAGGTTCCCACATTGATGAAACAGCTTTGTCTCTTTTGATCTCTTTTGATCTTGATGCTTCAT
  GCTATGCTACCGTTTGTCTGAAGGAAATAATGAAGCATGACGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233952 representing NM_001271826
  Red=Cloning site Green=Tags(s)
  
  MFEAIGFLAIKLGVIPSDFSYAGLKDKKAITYQAMVVRKVTPERLKNIEKEIEKKRMNVFNIRSVDDSLR
  LGQLKGNHFDIVIRNLKKQINDSANLRERIMEAIENVKKKGFVNYYGPQRFGKGRKVHTDQIGLALLKNE
  MMKAIKLFLTPEDLDDPVNRAKKYFLQTEDAKGTLSLMPEFKVRERALLEALHRFGMTEEGCIQAWFSLP
  HSMRIFYVHAYTSKIWNEAVSYRLETYGARVVQGDLVCLDEDIDDENFPNSKIHLVTEEEGSANMYAIHQ
  VVLPVLGYNIQYPKNKVGQWYHDILSRDGLQTCRFKVPTLKLNIPGCYRQILKHPCNLSYQLMEDHDIDV
  KTKGSHIDETALSLLISFDLDASCYATVCLKEIMKHDV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271826
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271826.1, NP_001258755.1
• RefSeq Size: 3059
• RefSeq ORF: 1167
• Locus ID: 83448