ZNF195 (NM_001242841) Human Tagged ORF Clone
CAT#: RG234558
- TrueORF®
ZNF195 (GFP-tagged) - Homo sapiens zinc finger protein 195 (ZNF195), transcript variant 4
"NM_001242841" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | ZNF195 |
Synonyms | HRF1; ZNFP104 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234558 representing NM_001242841
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGTGCTCAGACTCTGTTGACGTTCAGGGATGTGGCCATAGAATTCTCCCTGGAGGAGTGGAAAT GCCTGGACCTCGCTCAGCAGAATTTGTACAGGGATGTGATGTTGGAGAACTACAGAAACTTGTTCTCCGT TGGTCTCACTGTCTGTAAGCCAGGCCTGATCACCTGCCTGGAGCAACGAAAAGAGCCCTGGAATGTGAAG AGACAGGAGGCAGCAGACGGACATCCAGAGATGGGATTTCACCATGCTACTCAGGCTTGTCTTGAACTCC TGGGCTCAAGCGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAACCACCGTGCCCA GCCAGGCCTCAATGTTTCTGTGGACAAATTCACTGCTATGTCTTCTCATTTTACCCAAGACCTTCTGCCA GAGCAGGGCATACAAGATGCATTCCCAAAAAGAATACTGAGAGGATATGGAAATTGTGGCCTTGATAATT TATATTTAAGGAAAGACTGGGAAAGTTTAGATGAGTGTAAGTTGCAAAAAGATTATAATGGACTTAACCA ATGTTCATCAACTACCCATAGCAAAATCTTTCAATATAATAAATATGTTAAAATCTTTGATAACTTTTCA AATTTACATAGACGTAATATAAGTAATACTGGAGAGAAACCTTTCAAATGTCAAGAATGTGGCAAATCCT TTCAAATGCTCTCATTCCTAACTGAACATCAGAAAATTCACACTGGAAAAAAATTCCAAAAATGTGGAGA ATGTGGCAAAACCTTTATCCAGTGCTCACACTTTACTGAACCTGAGAACATTGACACTGGAGAGAAACCT TACAAGTGTCAAGAATGTAACAACGTCATTAAAACTTGCTCAGTCCTTACTAAAAATAGAATTTATGCCG GAGGGGAACATTACAGATGTGAAGAATTTGGCAAAGTATTTAACCAGTGCTCCCACCTTACTGAACATGA GCATGGTACTGAGGAAAAACCCTGCAAATATGAAGAGTGCAGCAGTGTCTTTATCTCTTGCTCAAGCCTT TCTAATCAACAGATGATTCTTGCTGGAGAGAAGCTCTCCAAATGTGAAACATGGTACAAAGGTTTTAACC ACAGCCCAAATCCTTCCAAACACCAGAGAAATGAGATTGGAGGGAAACCTTTCAAATGTGAGGAATGTGA CAGCATCTTCAAGTGGTTCTCAGACCTTACTAAACATAAGAGAATTCACACTGGTGAGAAACCATACAAA TGTGACGAATGTGGGAAAGCCTATACACAGTCCTCACACCTCAGTGAACACAGGAGGATTCACACCGGAG AGAAACCCTACCAATGTGAAGAATGTGGGAAGGTCTTCAGAACTTGCTCAAGCCTTTCTAACCATAAGAG AACTCATTCTGAAGAAAAACCCTACACGTGTGAAGAATGTGGCAACATCTTTAAGCAGTTATCAGACCTC ACTAAGCATAAGAAAACCCATACTGGAGAGAAGCCCTACAAATGTGACGAATGTGGAAAAAACTTTACCC AGTCCTCCAACCTTATTGTACATAAGAGAATTCATACTGGAGAGAAACCCTACAAGTGTGAAGAATGTGG CAGAGTCTTCATGTGGTTCTCAGACATTACCAAACATAAGAAAACCCATACTGGAGAGAAACCCTACAAA TGTGACGAATGTGGAAAAAACTTTACCCAGTCCTCAAACCTTATTGTACATAAGAGAATTCATACTGGAG AGAAACCCTACAAGTGTGAAAAGTGTGGCAAAGCCTTCACCCAGTTCTCACACCTGACTGTACATGAAAG CATTCATACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234558 representing NM_001242841
Red=Cloning site Green=Tags(s) MAGAQTLLTFRDVAIEFSLEEWKCLDLAQQNLYRDVMLENYRNLFSVGLTVCKPGLITCLEQRKEPWNVK RQEAADGHPEMGFHHATQACLELLGSSDLPASASQSAGITGVNHRAQPGLNVSVDKFTAMSSHFTQDLLP EQGIQDAFPKRILRGYGNCGLDNLYLRKDWESLDECKLQKDYNGLNQCSSTTHSKIFQYNKYVKIFDNFS NLHRRNISNTGEKPFKCQECGKSFQMLSFLTEHQKIHTGKKFQKCGECGKTFIQCSHFTEPENIDTGEKP YKCQECNNVIKTCSVLTKNRIYAGGEHYRCEEFGKVFNQCSHLTEHEHGTEEKPCKYEECSSVFISCSSL SNQQMILAGEKLSKCETWYKGFNHSPNPSKHQRNEIGGKPFKCEECDSIFKWFSDLTKHKRIHTGEKPYK CDECGKAYTQSSHLSEHRRIHTGEKPYQCEECGKVFRTCSSLSNHKRTHSEEKPYTCEECGNIFKQLSDL TKHKKTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEECGRVFMWFSDITKHKKTHTGEKPYK CDECGKNFTQSSNLIVHKRIHTGEKPYKCEKCGKAFTQFSHLTVHESIHT TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001242841 |
ORF Size | 1830 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001242841.1, NP_001229770.1 |
RefSeq Size | 3293 bp |
RefSeq ORF | 1833 bp |
Locus ID | 7748 |
Cytogenetics | 11p15.4 |
Protein Families | Transcription Factors |
Gene Summary | 'This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]' |
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