ZNF195 (NM_001242841) Human Tagged ORF Clone

CAT#: RG234558

ZNF195 (GFP-tagged) - Homo sapiens zinc finger protein 195 (ZNF195), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF195
• Synonyms: HRF1; ZNFP104
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234558 representing NM_001242841
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGTGCTCAGACTCTGTTGACGTTCAGGGATGTGGCCATAGAATTCTCCCTGGAGGAGTGGAAAT
  GCCTGGACCTCGCTCAGCAGAATTTGTACAGGGATGTGATGTTGGAGAACTACAGAAACTTGTTCTCCGT
  TGGTCTCACTGTCTGTAAGCCAGGCCTGATCACCTGCCTGGAGCAACGAAAAGAGCCCTGGAATGTGAAG
  AGACAGGAGGCAGCAGACGGACATCCAGAGATGGGATTTCACCATGCTACTCAGGCTTGTCTTGAACTCC
  TGGGCTCAAGCGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAACCACCGTGCCCA
  GCCAGGCCTCAATGTTTCTGTGGACAAATTCACTGCTATGTCTTCTCATTTTACCCAAGACCTTCTGCCA
  GAGCAGGGCATACAAGATGCATTCCCAAAAAGAATACTGAGAGGATATGGAAATTGTGGCCTTGATAATT
  TATATTTAAGGAAAGACTGGGAAAGTTTAGATGAGTGTAAGTTGCAAAAAGATTATAATGGACTTAACCA
  ATGTTCATCAACTACCCATAGCAAAATCTTTCAATATAATAAATATGTTAAAATCTTTGATAACTTTTCA
  AATTTACATAGACGTAATATAAGTAATACTGGAGAGAAACCTTTCAAATGTCAAGAATGTGGCAAATCCT
  TTCAAATGCTCTCATTCCTAACTGAACATCAGAAAATTCACACTGGAAAAAAATTCCAAAAATGTGGAGA
  ATGTGGCAAAACCTTTATCCAGTGCTCACACTTTACTGAACCTGAGAACATTGACACTGGAGAGAAACCT
  TACAAGTGTCAAGAATGTAACAACGTCATTAAAACTTGCTCAGTCCTTACTAAAAATAGAATTTATGCCG
  GAGGGGAACATTACAGATGTGAAGAATTTGGCAAAGTATTTAACCAGTGCTCCCACCTTACTGAACATGA
  GCATGGTACTGAGGAAAAACCCTGCAAATATGAAGAGTGCAGCAGTGTCTTTATCTCTTGCTCAAGCCTT
  TCTAATCAACAGATGATTCTTGCTGGAGAGAAGCTCTCCAAATGTGAAACATGGTACAAAGGTTTTAACC
  ACAGCCCAAATCCTTCCAAACACCAGAGAAATGAGATTGGAGGGAAACCTTTCAAATGTGAGGAATGTGA
  CAGCATCTTCAAGTGGTTCTCAGACCTTACTAAACATAAGAGAATTCACACTGGTGAGAAACCATACAAA
  TGTGACGAATGTGGGAAAGCCTATACACAGTCCTCACACCTCAGTGAACACAGGAGGATTCACACCGGAG
  AGAAACCCTACCAATGTGAAGAATGTGGGAAGGTCTTCAGAACTTGCTCAAGCCTTTCTAACCATAAGAG
  AACTCATTCTGAAGAAAAACCCTACACGTGTGAAGAATGTGGCAACATCTTTAAGCAGTTATCAGACCTC
  ACTAAGCATAAGAAAACCCATACTGGAGAGAAGCCCTACAAATGTGACGAATGTGGAAAAAACTTTACCC
  AGTCCTCCAACCTTATTGTACATAAGAGAATTCATACTGGAGAGAAACCCTACAAGTGTGAAGAATGTGG
  CAGAGTCTTCATGTGGTTCTCAGACATTACCAAACATAAGAAAACCCATACTGGAGAGAAACCCTACAAA
  TGTGACGAATGTGGAAAAAACTTTACCCAGTCCTCAAACCTTATTGTACATAAGAGAATTCATACTGGAG
  AGAAACCCTACAAGTGTGAAAAGTGTGGCAAAGCCTTCACCCAGTTCTCACACCTGACTGTACATGAAAG
  CATTCATACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234558 representing NM_001242841
  Red=Cloning site Green=Tags(s)
  
  MAGAQTLLTFRDVAIEFSLEEWKCLDLAQQNLYRDVMLENYRNLFSVGLTVCKPGLITCLEQRKEPWNVK
  RQEAADGHPEMGFHHATQACLELLGSSDLPASASQSAGITGVNHRAQPGLNVSVDKFTAMSSHFTQDLLP
  EQGIQDAFPKRILRGYGNCGLDNLYLRKDWESLDECKLQKDYNGLNQCSSTTHSKIFQYNKYVKIFDNFS
  NLHRRNISNTGEKPFKCQECGKSFQMLSFLTEHQKIHTGKKFQKCGECGKTFIQCSHFTEPENIDTGEKP
  YKCQECNNVIKTCSVLTKNRIYAGGEHYRCEEFGKVFNQCSHLTEHEHGTEEKPCKYEECSSVFISCSSL
  SNQQMILAGEKLSKCETWYKGFNHSPNPSKHQRNEIGGKPFKCEECDSIFKWFSDLTKHKRIHTGEKPYK
  CDECGKAYTQSSHLSEHRRIHTGEKPYQCEECGKVFRTCSSLSNHKRTHSEEKPYTCEECGNIFKQLSDL
  TKHKKTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEECGRVFMWFSDITKHKKTHTGEKPYK
  CDECGKNFTQSSNLIVHKRIHTGEKPYKCEKCGKAFTQFSHLTVHESIHT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242841
• ORF Size: 1830 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242841.1, NP_001229770.1
• RefSeq Size: 3293 bp
• RefSeq ORF: 1833 bp
• Locus ID: 7748
• Cytogenetics: 11p15.4
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]'