CLPB (NM_001258393) Human Tagged ORF Clone

CAT#: RG234652

CLPB (GFP-tagged) - Homo sapiens ClpB caseinolytic peptidase B homolog (E. coli) (CLPB), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CLPB
• Synonyms: ANKCLB; HSP78; MEGCANN; MGCA7; SKD3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234652 representing NM_001258393
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGGTCCCTGGTGTTGAGGAGAAAAGCACTGGCGCCACGGCTACTCCTCCGGCTGCTCAGGTCCC
  CAACGCTCCGGGGCCATGGAGGTGCTTCCGGCCGGAATGTGACTACTGGGAGTCTCGGGGAGCCGCAGTG
  GCTGAGGGTAGCCACCGGGGGGCGCCCTGGAACATCGCCGGCCTTGTTCTCCGGACGTGGGGCAGCCACC
  GGGGGGCGCCAGGGAGGACGCTTCGATACCAAATGCCTCGCGGCTGCCACTTGGGGACGCCTTCCTGGTC
  CCGAAGAAACACTCCCAGGACAGGACAGCTGGAACGGGGTCCCCAGCAGGGCCGGACTGGGCATGTGCGC
  CCTGGCCGCAGCGCTGGTGGTTCATTGCTACAGCAAGAGTCCGTCCAACAAGGATGCAGCCCTGTTGGAA
  GCTGCCCGTGCCAACAATATGCAAGAAGTCAGCAGTGTGGTACAGGTCCTGCTTGCTGCTGGGGCTGATC
  CAAACCTTGGAGATGATTTCAGCAGTGTTTACAAGACTGCCAAGGAACAGGGAATCCATTCTTTGGAAGT
  CCTGATCACCCGAGAGGATGACTTCAACAACAGGCTGAACAACCGCGCCAGTTTCAAGGGCTGCACGGCC
  TTGCACTATGCTGTTCTTGCTGATGACTACCGCACTGTCAAGGAGCTGCTTGATGGAGGAGCCAACCCCC
  TGCAGAGGAATGAAATGGGACACACACCCTTGGATTATGCCCGAGAAGGGGAAGTGATGAAGCTTCTGAG
  GACTTCTGAAGCCAAGTACCAAGAGAAGCAGCGGAAGCGTGAGGCTGAGGAGCGGCGCCGCTTCCCCCTG
  GAGCAGCGACTAAAGGAGCACATCATTGGCCAGGAGAGCGCCATCGCCACAGTGGGTGCTGCGATCCGGA
  GGAAGGAGAATGGCTGGTACGATGAAGAACACCCTCTGGTCTTCCTCTTCTTGGGATCATCTGGAATAGG
  AAAAACAGAGCTGGCCAAGCAGACAGCCAAATATATGCACAAAGATGCTAAAAAGGGCTTCATCAGGCTG
  GACATGTCCGAGTTCCAGGAGCGACACGAGGTGGCCAAGTTTATTGGGTCTCCACCAGGCTACGTTGGCC
  ATGAGGAGGGTGGCCAGCTGACCAAGAAGTTGAAGCAGTGCCCCAATGCTGTGGTGCTCTTTGATGAAGT
  AGACAAGGCCCATCCAGATGTGCTCACCATCATGCTGCAGCTGTTTGATGAGGGCCGGCTGACAGATGGA
  AAAGGGAAGACCATTGATTGCAAGGACGCCATCTTCATCATGACCTCCAATGTGGCCAGCGACGAGATCG
  CACAGCACGCGCTGCAGCTGAGGCAGGAAGCTTTGGAGATGAGCCGTAACCGTATTGCCGAAAACCTGGG
  GGATGTCCAGATAAGTGACAAGATCACCATCTCAAAGAACTTCAAGGAGAATGTGATTCGCCCTATCCTG
  AAAGCTCACTTCCGGAGGGATGAGTTTCTGGGACGGATCAATGAGATCGTCTACTTCCTCCCCTTCTGCC
  ACTCGGAGCTCATCCAACTCGTCAACAAGGAACTAAACTTCTGGGCCAAGAGAGCCAAGCAAAGGCACAA
  CATCACGCTGCTCTGGGACCGCGAGGTGGCAGATGTGCTGGTCGACGGCTACAATGTGCACTATGGCGCC
  CGCTCCATCAAACATGAGGTAGAACGCCGTGTGGTGAACCAGCTGGCAGCAGCCTATGAGCAGGACCTGC
  TGCCAGGGGGCTGTACTTTGCGCATCACGGTGGAGGACTCAGACAAGCAGCTACTCAAAAGCCCAGAACT
  GCCCTCACCCCAGGCTGAGAAGCGCCTCCCCAAGCTGCGTCTGGAGATCATCGACAAGGACAGCAAGACT
  CGCAGACTGGACATCCGGGCACCACTGCACCCTGAGAAGGTGTGCAACACCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234652 representing NM_001258393
  Red=Cloning site Green=Tags(s)
  
  MLGSLVLRRKALAPRLLLRLLRSPTLRGHGGASGRNVTTGSLGEPQWLRVATGGRPGTSPALFSGRGAAT
  GGRQGGRFDTKCLAAATWGRLPGPEETLPGQDSWNGVPSRAGLGMCALAAALVVHCYSKSPSNKDAALLE
  AARANNMQEVSSVVQVLLAAGADPNLGDDFSSVYKTAKEQGIHSLEVLITREDDFNNRLNNRASFKGCTA
  LHYAVLADDYRTVKELLDGGANPLQRNEMGHTPLDYAREGEVMKLLRTSEAKYQEKQRKREAEERRRFPL
  EQRLKEHIIGQESAIATVGAAIRRKENGWYDEEHPLVFLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRL
  DMSEFQERHEVAKFIGSPPGYVGHEEGGQLTKKLKQCPNAVVLFDEVDKAHPDVLTIMLQLFDEGRLTDG
  KGKTIDCKDAIFIMTSNVASDEIAQHALQLRQEALEMSRNRIAENLGDVQISDKITISKNFKENVIRPIL
  KAHFRRDEFLGRINEIVYFLPFCHSELIQLVNKELNFWAKRAKQRHNITLLWDREVADVLVDGYNVHYGA
  RSIKHEVERRVVNQLAAAYEQDLLPGGCTLRITVEDSDKQLLKSPELPSPQAEKRLPKLRLEIIDKDSKT
  RRLDIRAPLHPEKVCNTI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258393
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001258393.1, NP_001245322.1
• RefSeq Size: 3095
• RefSeq ORF: 1947
• Locus ID: 81570
• Gene Summary: This gene belongs to the ATP-ases associated with diverse cellular activities (AAA+) superfamily. Members of this superfamily form ring-shaped homo-hexamers and have highly conserved ATPase domains that are involved in various processes including DNA replication, protein degradation and reactivation of misfolded proteins. All members of this family hydrolyze ATP through their AAA+ domains and use the energy generated through ATP hydrolysis to exert mechanical force on their substrates. In addition to an AAA+ domain, the protein encoded by this gene contains a C-terminal D2 domain, which is characteristic of the AAA+ subfamily of Caseinolytic peptidases to which this protein belongs. It cooperates with Hsp70 in the disaggregation of protein aggregates. Allelic variants of this gene are associated with 3-methylglutaconic aciduria, which causes cataracts and neutropenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]