RAD54B (NM_001205263) Human Tagged ORF Clone

CAT#: RG234779

RAD54B (GFP-tagged) - Homo sapiens RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RAD54B
• Synonyms: RDH54
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234779 representing NM_001205263
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTGTGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGATGACTTCAGCAGTGGCAGGTGTT
  TTCAGCTTGGAGGAGGAAGTACTGCTATCTCGCATTCTTCTCAGGTTGCCAGGAAATGTTTCTCTAACCC
  TTTCAAAAGTGTTTGTAAACCAAGTTCAAAGGAAAATAGACAGAATGATTTCCAAAATTGCAAACCACGC
  CATGACCCATATACGCCAAATTCCCTCGTTATGCCACGACCAGATAAGAATCACCAGTGGGTATTCAATA
  AGAACTGTTTCCCTCTTGTGGATGTAGTGATTGATCCTTACCTTGTATATCATCTTCGACCACATCAGAA
  AGAAGGAATCATATTCCTTTATGAATGTGTAATGGGAATGAGAATGAATGGCAGATGTGGAGCTATTCTT
  GCTGATGAAATGGGTTTAGGGAAGACATTGCAATGTATTTCGCTCATCTGGACCCTGCAGTGTCAGGGAC
  CCTATGGAGGCAAGCCAGTAATAAAGAAGACACTAATTGTCACACCTGGAAGCTTGGTGAATAATTGGAA
  GAAAGAATTTCAAAAATGGCTAGGAAGTGAAAGGATCAAGATATTTACTGTTGATCAGGACCACAAAGTT
  GAAGAATTCATCAAGTCTATATTTTATTCTGTTCTTATTATCAGTTATGAAATGTTACTTCGTTCCCTGG
  ATCAAATTAAGAATATAAAATTTGATCTTCTAATCTGTGACGAGGGGCATCGTTTGAAGAACAGTGCCAT
  TAAGACAACTACAGCCCTCATTAGCCTTTCTTGTGAGAAAAGAATAATTCTAACTGGTACTCCAATTCAG
  AATGATCTGCAAGAATTTTTTGCATTAATTGATTTTGTAAATCCAGGAATATTAGGCTCTTTGTCATCTT
  ATAGGAAAATATATGAAGAACCCATCATTTTATCGAGAGAACCTTCTGCTTCTGAGGAAGAAAAGGAGTT
  AGGAGAAAGAAGAGCAGCTGAACTTACTTGCCTCACTGGACTCTTTATCCTTAGAAGAACCCAAGAAATT
  ATAAATAAATATCTCCCACCTAAAATAGAGAATGTTGTCTTTTGCCGACCAGGAGCACTACAGATTGAGC
  TTTATCGAAAGCTGTTAAATTCTCAGGTTGTCAGGTTCTGCCTTCAAGGGTTGTTGGAAAATAGTCCCCA
  TCTAATATGTATAGGAGCTCTTAAAAAACTGTGCAATCACCCCTGCCTTTTGTTCAACTCTATAAAGGAA
  AAGGAATGTAGCTCAACTTGTGATAAAAATGAAGAAAAGAGTCTATACAAAGGCTTGCTAAGTGTGTTTC
  CTGCTGACTACAACCCTCTCCTGTTTACTGAAAAGGAGTCAGGAAAACTACAGGTGTTGTCCAAGCTCTT
  AGCGGTTATCCACGAACTTCGACCTACTGAAAAGGTGGTGTTGGTATCCAACTATACACAAACCTTGAAC
  ATTTTACAAGAAGTATGTAAGCGTCATGGATATGCTTATACAAGACTTGATGGACAAACACCAATCTCTC
  AAAGGCAGCAGATTGTTGATGGCTTTAACAGTCAACACTCTTCTTTTTTTATTTTTTTGTTAAGTTCAAA
  AGCTGGTGGTGTAGGACTTAACCTCATTGGAGGATCTCACTTAATTCTCTATGACATTGATTGGAATCCA
  GCCACTGACATTCAGGCAATGTCTAGAGTATGGAGAGATGGTCAGAAATATCCTGTACATATTTACAGAC
  TCCTAACTACAGGTACAATAGAAGAAAAGATCTATCAAAGGCAGATCAGTAAGCAAGGTCTTTGTGGGGC
  AGTTGTCGACCTCACCAAGACATCTGAACATATTCAGTTTTCAGTAGAAGAACTTAAAAATTTGTTCACA
  TTACATGAAAGTTCAGATTGTGTTACTCATGATCTGCTTGACTGTGAGTGTACAGGAGAAGAAGTTCATA
  CAGGTGATTCGTTGGAAAAATTCATTGTCTCTAGAGATTGTCAGCTTGGTCCACATCACCAGAAATCTAA
  CTCCCTGAAACCTCTTTCTATGTCCCAGCTGAAGCAATGGAAACATTTTTCTGGAGATCATTTAAATCTT
  ACAGATCCTTTTCTTGAAAGAATAACAGAAAATGTGTCATTCATTTTTCAGAATATAACCACTCAAGCTA
  CTGGCACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234779 representing NM_001205263
  Red=Cloning site Green=Tags(s)
  
  MICGKEIEVMGVISPDDFSSGRCFQLGGGSTAISHSSQVARKCFSNPFKSVCKPSSKENRQNDFQNCKPR
  HDPYTPNSLVMPRPDKNHQWVFNKNCFPLVDVVIDPYLVYHLRPHQKEGIIFLYECVMGMRMNGRCGAIL
  ADEMGLGKTLQCISLIWTLQCQGPYGGKPVIKKTLIVTPGSLVNNWKKEFQKWLGSERIKIFTVDQDHKV
  EEFIKSIFYSVLIISYEMLLRSLDQIKNIKFDLLICDEGHRLKNSAIKTTTALISLSCEKRIILTGTPIQ
  NDLQEFFALIDFVNPGILGSLSSYRKIYEEPIILSREPSASEEEKELGERRAAELTCLTGLFILRRTQEI
  INKYLPPKIENVVFCRPGALQIELYRKLLNSQVVRFCLQGLLENSPHLICIGALKKLCNHPCLLFNSIKE
  KECSSTCDKNEEKSLYKGLLSVFPADYNPLLFTEKESGKLQVLSKLLAVIHELRPTEKVVLVSNYTQTLN
  ILQEVCKRHGYAYTRLDGQTPISQRQQIVDGFNSQHSSFFIFLLSSKAGGVGLNLIGGSHLILYDIDWNP
  ATDIQAMSRVWRDGQKYPVHIYRLLTTGTIEEKIYQRQISKQGLCGAVVDLTKTSEHIQFSVEELKNLFT
  LHESSDCVTHDLLDCECTGEEVHTGDSLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNL
  TDPFLERITENVSFIFQNITTQATGT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001205263
• ORF Size: 2178 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001205263.1, NP_001192192.1
• RefSeq Size: 3097
• RefSeq ORF: 2181
• Locus ID: 25788
• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination
• Gene Summary: The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008]