CTAGE5 (MIA2) (NM_001247988) Human Tagged ORF Clone

CAT#: RG234791

CTAGE5 (GFP-tagged) - Homo sapiens CTAGE family, member 5 (CTAGE5), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MIA2
• Synonyms: CTAGE5; MEA6; MGEA; MGEA6; MGEA11; TALI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234791 representing NM_001247988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACCAGATTCTAATCTTTATGGTTTTCCATGGGAATTGGTGATATGTGCAGCTGTTGTTGGATTTT
  TTGCTGTTCTCTTTTTTTTGTGGAGAAGTTTTAGATCGGTTAGGAGTCGGCTTTATGTGGGACGAGAGAA
  AAAGCTTGCTCTAATGCTTTCTGGACTAATTGAAGAAAAAAGTAAACTACTTGAAAAATTTAGCCTTGTT
  CAAAAAGAGTATGAAGGCTATGAAGTAGAGTCATCTTTAAAGGATGCCAGCTTTGAGAAGGAGGCAACAG
  AAGCACAAAGTTTGGAGGCAACCTGTGAAAAGCTGAACAGGTCCAATTCTGAACTTGAGGATGAAATACT
  CTGTCTAGAAAAAGAGTTAAAAGAAGAGAAATCCAAACATTCTGAACAAGATGAATTGATGGCGGATATT
  TCAAAAAGGATACAGTCTCTAGAAGATGAGTCAAAATCCCTCAAATCACAAGTAGCTGAAGCCAAAATGA
  CCTTCAAGATATTTCAAATGAATGAAGAACGACTGAAGATAGCAATAAAAGATGCTTTGAATGAAAATTC
  TCAACTTCAGGAAAGCCAGAAACAGCTTTTGCAAGAAGCTGAAGTATGGAAAGAACAAGTGAGTGAACTT
  AATAAACAGAAAGTAACATTTGAAGACTCCAAAGTACATGCAGAACAAGTTCTAAATGATAAAGAAAGTC
  ACATCAAGACTCTGACTGAACGCTTGTTAAAGATGAAAGATTGGGCTGCTATGCTTGGAGAAGACATAAC
  GGATGATGATAACTTGGAATTAGAAATGAACAGTGAATCGGAAAATGGTGCTTACTTAGATAATCCTCCA
  AAAGGAGCTTTGAAGAAACTGATTCATGCTGCTAAGTTAAATGCTTCTTTAAAAACCTTAGAAGGAGAAA
  GAAACCAAATTTATATTCAGTTGTCTGAAGTTGATAAAACAAAGGAAGAGCTTACAGAGCATATTAAAAA
  TCTTCAGACTGAACAAGCATCTTTGCAGTCAGAAAACACACATTTTGAAAATGAGAATCAGAAGCTTCAA
  CAGAAACTTAAAGTAATGACTGAATTATATCAAGAAAATGAAATGAAACTCCACAGGAAATTAACAGTAG
  AGGAAAATTATCGGTTAGAGAAAGAAGAGAAACTTTCTAAAGTAGATGAAAAGATCAGCCATGCCACTGA
  AGAGCTGGAGACCTATAGAAAGCGAGCCAAAGATCTTGAAGAAGAATTGGAGAGAACTATTCATTCTTAT
  CAAGGGCAGATTATTTCCCATGAGAAAAAAGCACATGATAATTGGTTGGCAGCTCGGAATGCTGAAAGAA
  ACCTCAATGATTTAAGGAAAGAAAATGCTCACAACAGACAAAAATTAACTGAAACAGAGCTTAAATTTGA
  ACTTTTAGAAAAAGATCCTTATGCACTCGATGTTCCAAATACAGCATTTGGCAGAGGCTCACGAGGCCCA
  GGGAATCCTCTGGACCATCAGATTACCAATGAAAGAGGAGAATCAAGCTGTGATAGGTTAACCGATCCTC
  ATAGGGCTCCCTCTGACACTGGGTCTCTGTCACCTCCATGGGACCAGGACCGTAGGATGATGTTTCCTCC
  GCCAGGACAATCATATCCTGATTCAGCCCTTCCTCCACAAAGGCAAGACAGATTTTGTTCTAATTCTGGT
  AGACTGTCTGGACCAGCAGAACTCAGAAGTTTTAATATGCCTTCTTTGGATAAAATGGATGGGTCAATGC
  CTTCAGAAATGGAATCCAGTAGAAATGATACCAAAGATGATCTTGGTAATTTAAATGTGCCTGATTCATC
  TCTCCCTGCTGAAAATGAAGCCACTGGCCCTGGCTTTGTTCCTCCACCTCTTGCTCCAATCAGAGGTCCA
  TTGTTTCCAGTGGATGCAAGAGGCCCATTCTTGAGAAGAGGACCTCCTTTCCCCCCACCTCCTCCAGGAG
  CCATGTTTGGAGCTTCTCGAGATTATTTTCCACCAGGGGATTTCCCAGGTCCACCACCTGCTCCATTTGC
  AATGAGAAATGTCTATCCACCGAGGGGTTTTCCTCCTTACCTTCCCCCAAGACCTGGATTTTTCCCCCCA
  CCCCCACATTCTGAAGGTAGAAGTGAGTTCCCCTCAGGTTTGATTCCACCTTCAAATGAGCCTGCTACTG
  AACATCCAGAACCACAGCAAGAAACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234791 representing NM_001247988
  Red=Cloning site Green=Tags(s)
  
  MRPDSNLYGFPWELVICAAVVGFFAVLFFLWRSFRSVRSRLYVGREKKLALMLSGLIEEKSKLLEKFSLV
  QKEYEGYEVESSLKDASFEKEATEAQSLEATCEKLNRSNSELEDEILCLEKELKEEKSKHSEQDELMADI
  SKRIQSLEDESKSLKSQVAEAKMTFKIFQMNEERLKIAIKDALNENSQLQESQKQLLQEAEVWKEQVSEL
  NKQKVTFEDSKVHAEQVLNDKESHIKTLTERLLKMKDWAAMLGEDITDDDNLELEMNSESENGAYLDNPP
  KGALKKLIHAAKLNASLKTLEGERNQIYIQLSEVDKTKEELTEHIKNLQTEQASLQSENTHFENENQKLQ
  QKLKVMTELYQENEMKLHRKLTVEENYRLEKEEKLSKVDEKISHATEELETYRKRAKDLEEELERTIHSY
  QGQIISHEKKAHDNWLAARNAERNLNDLRKENAHNRQKLTETELKFELLEKDPYALDVPNTAFGRGSRGP
  GNPLDHQITNERGESSCDRLTDPHRAPSDTGSLSPPWDQDRRMMFPPPGQSYPDSALPPQRQDRFCSNSG
  RLSGPAELRSFNMPSLDKMDGSMPSEMESSRNDTKDDLGNLNVPDSSLPAENEATGPGFVPPPLAPIRGP
  LFPVDARGPFLRRGPPFPPPPPGAMFGASRDYFPPGDFPGPPPAPFAMRNVYPPRGFPPYLPPRPGFFPP
  PPHSEGRSEFPSGLIPPSNEPATEHPEPQQET
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001247988
• ORF Size: 2196 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001247988.1, NP_001234917.1
• RefSeq Size: 3382 bp
• RefSeq ORF: 2199 bp
• Locus ID: 4253
• Cytogenetics: 14q21.1
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes s receptor in the endoplasmic reticulum, which plays a role in the export of large pre-chylomicrons and pre-very low density lipoproteins (pre-VLDLs). Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species (TANGO1-like or TALI). Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Sep 2016]'