BRD2 (NM_001199456) Human Tagged ORF Clone

CAT#: RG234837

BRD2 (GFP-tagged) - Homo sapiens bromodomain containing 2 (BRD2), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BRD2
• Synonyms: BRD2-IT1; D6S113E; FSH; FSRG1; NAT; O27.1.1; RING3; RNF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234837 representing NM_001199456
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCGGTGCCTGCTTTGCAACTTACCCCTGCCAACCCACCACCCCCGGAGGTGTCCAATCCCAAAA
  AGCCAGGACGAGTTACCAACCAGCTGCAATACCTACACAAGGTAGTGATGAAGGCTCTGTGGAAACATCA
  GTTCGCATGGCCATTCCGGCAGCCTGTGGATGCTGTCAAACTGGGTCTACCGGATTATCACAAAATTATA
  AAACAGCCTATGGACATGGGTACTATTAAGAGGAGACTTGAAAACAATTATTATTGGGCTGCTTCAGAGT
  GTATGCAAGATTTTAATACCATGTTCACCAACTGTTACATTTACAACAAGCCCACTGATGATATTGTCCT
  AATGGCACAAACGCTGGAAAAGATATTCCTACAGAAGGTTGCATCAATGCCACAAGAAGAACAAGAGCTG
  GTAGTGACCATCCCTAAGAACAGCCACAAGAAGGGGGCCAAGTTGGCAGCGCTCCAGGGCAGTGTTACCA
  GTGCCCATCAGGTGCCTGCCGTCTCTTCTGTGTCACACACAGCCCTGTATACTCCTCCACCTGAGATACC
  TACCACTGTCCTCAACATTCCCCACCCATCAGTCATTTCCTCTCCACTTCTCAAGTCCTTGCACTCTGCT
  GGACCCCCGCTCCTTGCTGTTACTGCAGCTCCTCCAGCCCAGCCCCTTGCCAAGAAAAAAGGCGTAAAGC
  GGAAAGCAGATACTACCACCCCTACACCTACAGCCATCTTGGCTCCTGGTTCTCCAGCTAGCCCTCCTGG
  GAGTCTTGAGCCTAAGGCAGCACGGCTTCCCCCTATGCGTAGAGAGAGTGGTCGCCCCATCAAGCCCCCA
  CGCAAAGACTTGCCTGACTCTCAGCAACAACACCAGAGCTCTAAGAAAGGAAAGCTTTCAGAACAGTTAA
  AACATTGCAATGGCATTTTGAAGGAGTTACTCTCTAAGAAGCATGCTGCCTATGCTTGGCCTTTCTATAA
  ACCAGTGGATGCTTCTGCACTTGGCCTGCATGACTACCATGACATCATTAAGCACCCCATGGACCTCAGC
  ACTGTCAAGCGGAAGATGGAGAACCGTGATTACCGGGATGCACAGGAGTTTGCTGCTGATGTACGGCTTA
  TGTTCTCCAACTGCTATAAGTACAATCCCCCAGATCACGATGTTGTGGCAATGGCACGAAAGCTACAGGA
  TGTATTTGAGTTCCGTTATGCCAAGATGCCAGATGAACCACTAGAACCAGGGCCTTTACCAGTCTCTACT
  GCCATGCCCCCTGGCTTGGCCAAATCGTCTTCAGAGTCCTCCAGTGAGGAAAGTAGCAGTGAGAGCTCCT
  CTGAGGAAGAGGAGGAGGAAGATGAGGAGGACGAGGAGGAAGAAGAGAGTGAAAGCTCAGACTCAGAGGA
  AGAAAGGGCTCATCGCTTAGCAGAACTACAGGAACAGCTTCGGGCAGTACATGAACAACTGGCTGCTCTG
  TCCCAGGGTCCAATATCCAAGCCCAAGAGGAAAAGAGAGAAAAAAGAGAAAAAGAAGAAACGGAAGGCAG
  AGAAGCATCGAGGCCGAGCTGGGGCCGATGAAGATGACAAGGGGCCTAGGGCACCCCGCCCACCTCAACC
  TAAGAAGTCCAAGAAAGCAAGTGGCAGTGGGGGTGGCAGTGCTGCTTTAGGCCCTTCTGGCTTTGGACCT
  TCTGGAGGAAGTGGCACCAAGCTCCCCAAAAAGGCCACAAAGACAGCCCCACCTGCCCTGCCTACAGGTT
  ATGATTCAGAGGAGGAGGAAGAGAGCAGGCCCATGAGTTACGATGAGAAGCGGCAGCTGAGCCTGGACAT
  CAACAAATTACCTGGGGAGAAGCTGGGCCGAGTTGTGCATATAATCCAAGCCAGGGAGCCCTCTTTACGT
  GATTCAAACCCAGAAGAGATTGAGATTGATTTTGAAACACTCAAGCCATCCACACTTAGAGAGCTTGAGC
  GCTATGTCCTTTCCTGCCTACGTAAGAAACCCCGGAAGCCCTACACCATTAAGAAGCCTGTGGGAAAGAC
  AAAGGAGGAACTGGCTTTGGAGAAAAAGCGGGAATTAGAAAAGCGGTTACAAGATGTCAGCGGACAGCTC
  AATTCTACTAAAAAGCCCCCCAAGAAAGCGAATGAGAAAACAGAGTCATCCTCTGCACAGCAAGTAGCAG
  TGTCACGCCTTAGCGCTTCCAGCTCCAGCTCAGATTCCAGCTCCTCCTCTTCCTCGTCGTCGTCTTCAGA
  CACCAGTGATTCAGACTCAGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234837 representing NM_001199456
  Red=Cloning site Green=Tags(s)
  
  MASVPALQLTPANPPPPEVSNPKKPGRVTNQLQYLHKVVMKALWKHQFAWPFRQPVDAVKLGLPDYHKII
  KQPMDMGTIKRRLENNYYWAASECMQDFNTMFTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQEL
  VVTIPKNSHKKGAKLAALQGSVTSAHQVPAVSSVSHTALYTPPPEIPTTVLNIPHPSVISSPLLKSLHSA
  GPPLLAVTAAPPAQPLAKKKGVKRKADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPP
  RKDLPDSQQQHQSSKKGKLSEQLKHCNGILKELLSKKHAAYAWPFYKPVDASALGLHDYHDIIKHPMDLS
  TVKRKMENRDYRDAQEFAADVRLMFSNCYKYNPPDHDVVAMARKLQDVFEFRYAKMPDEPLEPGPLPVST
  AMPPGLAKSSSESSSEESSSESSSEEEEEEDEEDEEEEESESSDSEEERAHRLAELQEQLRAVHEQLAAL
  SQGPISKPKRKREKKEKKKKRKAEKHRGRAGADEDDKGPRAPRPPQPKKSKKASGSGGGSAALGPSGFGP
  SGGSGTKLPKKATKTAPPALPTGYDSEEEEESRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAREPSLR
  DSNPEEIEIDFETLKPSTLRELERYVLSCLRKKPRKPYTIKKPVGKTKEELALEKKRELEKRLQDVSGQL
  NSTKKPPKKANEKTESSSAQQVAVSRLSASSSSSDSSSSSSSSSSSDTSDSDSG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199456
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199456.1, NP_001186385.1
• RefSeq Size: 3210 bp
• RefSeq ORF: 2265 bp
• Locus ID: 6046
• Cytogenetics: 6p21.32
• Protein Families: Protein Kinase
• Gene Summary: 'This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010]'