PKD2L1 (NM_001253837) Human Tagged ORF Clone

CAT#: RG234845

PKD2L1 (GFP-tagged) - Homo sapiens polycystic kidney disease 2-like 1 (PKD2L1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PKD2L1
• Synonyms: PCL; PKD2L; PKDL; TRPP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234845 representing NM_001253837
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCCAGGAGACGGCATACAGGACCCAGGTGTCCAGCTGCTGCCTCCATATCTGTCAAGGCATCAG
  AGGCATCTCTCCCATGCCCCTTAGGACTTTGGGGAACAACCCTGACTGAGAACACAGCTGAGAACCGGGA
  ACTTTATATCAAGACCACCCTGAGGGAGCTGTTGGTATATATTGTGTTCCTGGTGGACATCTGTCTACTG
  ACCTATGGAATGACAAGCTCCAGTGCTTATTACTACACCAAAGTGATGTCTGAGCTCTTCTTACATACTC
  CATCAGACACTGGAGTCTCCTTTCAGGCCATCAGCAGCATGGCGGACTTCTGGGATTTTGCCCAGGGCCC
  ACTACTGGACAGTTTGTATTGGACCAAATGGTACAACAACCAGAGCCTGGGCCATGGCTCCCACTCCTTC
  ATCTACTATGAGAACATGCTGCTGGGGGTTCCGAGGCTGCGGCAGCTAAAGGTCCGCAATGACTCCTGTG
  TGGTGCATGAAGACTTCCGGGAGGACATTCTGAGCTGCTATGATGTCTACTCTCCAGACAAAGAAGAACA
  ACTCCCCTTTGGGCCCTTCAATGGCACAGCGTGGACATACCACTCGCAGGATGAGTTGGGGGGCTTCTCC
  CACTGGGGCAGGCTCACAAGCTACAGCGGAGGTGGCTACTACCTGGACCTTCCAGGATCCCGACAGGGTA
  GTGCAGAGGCTCTCCGGGCCCTTCAGGAGGGGCTGTGGCTGGACAGGGGCACTCGAGTGGTGTTCATCGA
  CTTCTCAGTCTACAATGCCAATATCAATCTTTTCTGTGTCCTGAGGCTGGTGGTGGAGTTTCCAGCTACA
  GGAGGTGCCATCCCATCCTGGCAAATCCGCACAGTCAAGCTGATCCGCTATGTCAGCAACTGGGACTTCT
  TTATCGTTGGCTGTGAGGTCATCTTCTGCGTCTTCATCTTCTACTATGTGGTGGAAGAGATCCTGGAGCT
  CCACATTCACCGGCTTCGCTACCTCAGCAGCATCTGGAACATACTGGACCTGGTGGTCATCTTGCTCTCC
  ATTGTGGCTGTGGGCTTCCACATATTCCGAACCCTCGAGGTGAATCGGCTCATGGGGAAGCTCCTGCAGC
  AGCCAAACACGTATGCAGACTTTGAGTTCCTCGCCTTCTGGCAGACACAGTACAACAACATGAATGCTGT
  CAACCTCTTCTTCGCCTGGATCAAGATATTCAAGTACATCAGCTTCAACAAAACCATGACCCAGCTCTCC
  TCCACGCTGGCCCGCTGTGCCAAGGACATCCTGGGCTTCGCCGTCATGTTCTTCATTGTTTTCTTCGCCT
  ATGCCCAACTCGGCTACCTGCTTTTCGGGACCCAAGTGGAAAACTTTAGCACTTTCATCAAGTGCATTTT
  CACTCAGTTCCGGATAATCCTCGGGGACTTTGACTACAATGCTATCGACAATGCCAACCGCATCCTGGGC
  CCTGCCTACTTTGTCACCTATGTCTTCTTCGTCTTCTTCGTGCTCCTGAACATGTTCCTGGCCATCATCA
  ATGACACATATTCAGAGGTCAAGGAGGAGCTGGCTGGACAGAAGGATGAGCTGCAACTTTCTGACCTCCT
  GAAACAGGGCTACAACAAGACCCTACTAAGACTGCGTCTGAGGAAGGAGAGGGTTTCGGATGTGCAGAAG
  GTCCTGCAGGGTGGGGAGCAGGAGATCCAGTTTGAGGATTTCACCAACACCTTAAGGGAACTGGGACACG
  CAGAGCATGAAATCACTGAGCTCACGGCCACCTTCACCAAGTTTGACAGAGATGGGAATCGTATTCTGGA
  TGAGAAGGAACAGGAAAAAATGCGACAGGACCTGGAGGAAGAGAGGGTGGCCCTCAACACTGAGATTGAG
  AAACTAGGCCGATCTATTGTGAGCAGCCCACAAGGCAAATCGGGTCCAGAGGCTGCCAGAGCAGGAGGCT
  GGGTTTCAGGAGAAGAATTCTACATGCTCACAAGGAGAGTTCTGCAGCTGGAGACTGTCCTGGAAGGAGT
  AGTGTCCCAGATTGATGCTGTAGGCTCAAAGCTGAAAATGCTGGAGAGGAAGGGGTGGCTGGCTCCCTCC
  CCAGGCGTGAAGGAACAAGCTATTTGGAAGCACCCGCAGCCAGCCCCAGCTGTGACCCCAGACCCCTGGG
  GAGTCCAGGGTGGGCAGGAGAGTGAGGTTCCCTATAAAAGAGAAGAGGAAGCCTTAGAGGAGAGGAGACT
  CTCCCGTGGTGAGATTCCAACGTTGCAGAGGAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234845 representing NM_001253837
  Red=Cloning site Green=Tags(s)
  
  MNPRRRHTGPRCPAAASISVKASEASLPCPLGLWGTTLTENTAENRELYIKTTLRELLVYIVFLVDICLL
  TYGMTSSSAYYYTKVMSELFLHTPSDTGVSFQAISSMADFWDFAQGPLLDSLYWTKWYNNQSLGHGSHSF
  IYYENMLLGVPRLRQLKVRNDSCVVHEDFREDILSCYDVYSPDKEEQLPFGPFNGTAWTYHSQDELGGFS
  HWGRLTSYSGGGYYLDLPGSRQGSAEALRALQEGLWLDRGTRVVFIDFSVYNANINLFCVLRLVVEFPAT
  GGAIPSWQIRTVKLIRYVSNWDFFIVGCEVIFCVFIFYYVVEEILELHIHRLRYLSSIWNILDLVVILLS
  IVAVGFHIFRTLEVNRLMGKLLQQPNTYADFEFLAFWQTQYNNMNAVNLFFAWIKIFKYISFNKTMTQLS
  STLARCAKDILGFAVMFFIVFFAYAQLGYLLFGTQVENFSTFIKCIFTQFRIILGDFDYNAIDNANRILG
  PAYFVTYVFFVFFVLLNMFLAIINDTYSEVKEELAGQKDELQLSDLLKQGYNKTLLRLRLRKERVSDVQK
  VLQGGEQEIQFEDFTNTLRELGHAEHEITELTATFTKFDRDGNRILDEKEQEKMRQDLEEERVALNTEIE
  KLGRSIVSSPQGKSGPEAARAGGWVSGEEFYMLTRRVLQLETVLEGVVSQIDAVGSKLKMLERKGWLAPS
  PGVKEQAIWKHPQPAPAVTPDPWGVQGGQESEVPYKREEEALEERRLSRGEIPTLQRS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253837
• ORF Size: 2274 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001253837.1, NP_001240766.1
• RefSeq Size: 3082
• RefSeq ORF: 2277
• Locus ID: 9033
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: This gene encodes a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. This protein functions as a calcium-regulated nonselective cation channel. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]