CLCN3 (NM_001243372) Human Tagged ORF Clone

CAT#: RG234881

CLCN3 (GFP-tagged) - Homo sapiens chloride channel, voltage-sensitive 3 (CLCN3), transcript variant a

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CLCN3
• Synonyms: ClC-3; CLC3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234881 representing NM_001243372
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGAGTCTGAGCAGCTGTTCCATAGAGGCTACTATAGAAACAGCTACAACAGTATAACAAGTGCAAGTA
  GTGATGAGGAACTTTTAGATGGAGCAGGTGTTATTATGGACTTTCAAACATCTGAAGATGACAATTTATT
  AGATGGTGACACTGCAGTTGGAACTCATTATACAATGACAAATGGAGGCAGCATTAACAGTTCTACACAT
  TTACTGGATCTTTTGGATGAACCAATTCCAGGTGTTGGTACATATGATGATTTCCATACTATTGATTGGG
  TGCGAGAAAAATGTAAAGACAGAGAAAGGCATAGACGGATCAACAGCAAAAAGAAAGAATCAGCATGGGA
  AATGACAAAAAGTTTGTATGATGCGTGGTCAGGATGGCTAGTAGTAACACTAACAGGATTGGCATCAGGG
  GCACTGGCCGGATTAATAGACATTGCTGCCGATTGGATGACTGACCTAAAGGAGGGCATTTGCCTTAGTG
  CGTTGTGGTACAACCACGAACAGTGCTGTTGGGGATCTAATGAAACAACATTTGAAGAGAGGGATAAATG
  TCCACAGTGGAAAACATGGGCAGAATTAATCATAGGTCAAGCAGAGGGTCCTGGTTCTTATATCATGAAC
  TACATAATGTACATCTTCTGGGCCTTGAGTTTTGCCTTTCTTGCAGTTTCCCTGGTAAAGGTATTTGCTC
  CATATGCCTGTGGCTCTGGAATTCCAGAGATTAAAACTATTTTAAGTGGATTCATCATCAGAGGTTACTT
  GGGAAAATGGACTTTAATGATTAAAACCATCACATTAGTCCTGGCTGTGGCATCAGGTTTGAGTTTAGGA
  AAAGAAGGTCCCCTGGTACATGTTGCCTGTTGCTGCGGAAATATCTTTTCCTACCTCTTTCCAAAGTATA
  GCACAAACGAAGCTAAAAAAAGGGAGGTTAGCTATTATTTTCCTCTCAAAACTTTATGGAGATCATTTTT
  TGCTGCTTTAGTGGCTGCATTTGTTTTGAGGTCCATCAATCCATTTGGTAACAGCCGTCTGGTCCTTTTT
  TATGTGGAGTATCATACACCATGGTACCTTTTTGAACTGTTTCCTTTTATTCTTCTAGGGGTATTTGGAG
  GGCTTTGGGGAGCCTTTTTCATTAGGGCAAATATTGCCTGGTGTCGTCGACGCAAGTCCACGAAATTTGG
  AAAGTATCCCGTTCTGGAAGTCATTATTGTTGCAGCCATTACTGCTGTGATAGCCTTCCCTAATCCATAC
  ACTAGGCTAAACACCAGTGAACTGATCAAAGAGCTTTTTACAGACTGTGGTCCCCTGGAATCCTCTTCTC
  TTTGTGACTACAGAAATGACATGAATGCCAGTAAAATTGTCGATGACATTCCTGATCGTCCAGCAGGCAT
  TGGAGTATATTCAGCTATATGGCAGTTATGCCTGGCACTCATATTTAAAATCATAATGACAGTATTCACT
  TTTGGCATCAAGGTTCCATCAGGCTTGTTCATCCCCAGCATGGCCATTGGAGCGATCGCAGGAAGGATTG
  TGGGGATTGCGGTGGAGCAGCTTGCCTACTATCACCACGACTGGTTTATCTTTAAGGAGTGGTGTGAGGT
  CGGGGCTGATTGCATTACACCTGGCCTTTATGCCATGGTTGGTGCTGCTGCATGCTTAGGTGGTGTGACA
  AGAATGACTGTCTCCCTGGTGGTTATTGTTTTTGAGCTTACTGGAGGCTTGGAATATATTGTTCCCCTTA
  TGGCTGCAGTCATGACCAGTAAATGGGTTGGAGATGCCTTTGGCAGGGAAGGCATTTATGAAGCACACAT
  CCGATTAAATGGATACCCTTTCTTGGATGCAAAAGAAGAATTCACTCATACCACCCTGGCTGCTGACGTT
  ATGAGACCTCGAAGGAATGATCCTCCCTTAGCTGTCCTGACACAGGACAATATGACAGTGGATGATATAG
  AAAACATGATTAATGAAACCAGCTACAATGGATTTCCTGTCATAATGTCAAAAGAATCTCAGAGATTAGT
  GGGATTTGCCCTCAGAAGAGACCTGACAATTGCAATAGAAAGTGCCAGGAAAAAACAAGAAGGTATCGTT
  GGCAGTTCTCGGGTGTGTTTTGCACAGCACACCCCATCTCTTCCAGCAGAAAGTCCTCGGCCATTGAAGC
  TTCGAAGCATTCTTGACATGAGCCCTTTTACAGTGACAGACCACACCCCAATGGAGATCGTGGTGGATAT
  TTTCCGAAAGCTGGGACTGAGGCAGTGCCTTGTAACTCACAATGGGCGCCTCCTTGGCATTATAACAAAA
  AAAGATATCCTCCGGCATATGGCCCAGACGGCAAACCAAGACCCCGCTTCAATAATGTTCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234881 representing NM_001243372
  Red=Cloning site Green=Tags(s)
  
  MESEQLFHRGYYRNSYNSITSASSDEELLDGAGVIMDFQTSEDDNLLDGDTAVGTHYTMTNGGSINSSTH
  LLDLLDEPIPGVGTYDDFHTIDWVREKCKDRERHRRINSKKKESAWEMTKSLYDAWSGWLVVTLTGLASG
  ALAGLIDIAADWMTDLKEGICLSALWYNHEQCCWGSNETTFEERDKCPQWKTWAELIIGQAEGPGSYIMN
  YIMYIFWALSFAFLAVSLVKVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLMIKTITLVLAVASGLSLG
  KEGPLVHVACCCGNIFSYLFPKYSTNEAKKREVSYYFPLKTLWRSFFAALVAAFVLRSINPFGNSRLVLF
  YVEYHTPWYLFELFPFILLGVFGGLWGAFFIRANIAWCRRRKSTKFGKYPVLEVIIVAAITAVIAFPNPY
  TRLNTSELIKELFTDCGPLESSSLCDYRNDMNASKIVDDIPDRPAGIGVYSAIWQLCLALIFKIIMTVFT
  FGIKVPSGLFIPSMAIGAIAGRIVGIAVEQLAYYHHDWFIFKEWCEVGADCITPGLYAMVGAAACLGGVT
  RMTVSLVVIVFELTGGLEYIVPLMAAVMTSKWVGDAFGREGIYEAHIRLNGYPFLDAKEEFTHTTLAADV
  MRPRRNDPPLAVLTQDNMTVDDIENMINETSYNGFPVIMSKESQRLVGFALRRDLTIAIESARKKQEGIV
  GSSRVCFAQHTPSLPAESPRPLKLRSILDMSPFTVTDHTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITK
  KDILRHMAQTANQDPASIMFN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243372
• ORF Size: 2373 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001243372.1, NP_001230301.1
• RefSeq Size: 6142
• RefSeq ORF: 2376
• Locus ID: 1182
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]