GRIA1 (NM_001258020) Human Tagged ORF Clone

CAT#: RG234917

GRIA1 (GFP-tagged) - Homo sapiens glutamate receptor, ionotropic, AMPA 1 (GRIA1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GRIA1
• Synonyms: GluA1; GLUH1; GLUR1; GLURA; HBGR1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234917 representing NM_001258020
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACCTCCTTTTGTGGGGCCCTCCACGTCTGCTTCATTACGCCGAGCTTTCCCGTTGATACATCCA
  ATCAGTTTGTCCTTCAGCTGCGCCCTGAACTGCAGGATGCCCTCATCAGCATCATTGACCATTACAAGTG
  GCAGAAATTTGTCTACATTTATGATGCCGACCGGGGCTTATCCGTCCTGCAGAAAGTCCTGGATACAGCT
  GCTGAGAAGAACTGGCAGGTGACAGCAGTCAACATTTTGACAACCACAGAGGAGGGATACCGGATGCTCT
  TTCAGGACCTGGAGAAGAAAAAGGAGCGGCTGGTGGTGGTGGACTGTGAATCAGAACGCCTCAATGCTAT
  CTTGGGCCAGATTATAAAGCTAGAGAAGAATGGCATCGGCTACCACTACATTCTTGCAAATCTGGGCTTC
  ATGGACATTGACTTAAACAAATTCAAGGAGAGTGGCGCCAATGTGACAGGTTTCCAGCTGGTGAACTACA
  CAGACACTATTCCGGCCAAGATCATGCAGCAGTGGAAGAATAGTGATGCTCGAGACCACACACGGGTGGA
  CTGGAAGAGACCCAAGTACACCTCTGCGCTCACCTACGATGGGGTGAAGGTGATGGCTGAGGCTTTCCAG
  AGCCTGCGGAGGCAGAGAATTGATATATCTCGCCGGGGGAATGCTGGGGATTGTCTGGCTAACCCAGCTG
  TTCCCTGGGGCCAAGGGATCGACATCCAGAGAGCTCTGCAGCAGGTGCGATTTGAAGGTTTAACAGGAAA
  CGTGCAGTTTAATGAGAAAGGACGCCGGACCAACTACACGCTCCACGTGATTGAAATGAAACATGACGGC
  ATCCGAAAGATTGGTTACTGGAATGAAGATGATAAGTTTGTCCCTGCAGCCACCGATGCCCAAGCTGGGG
  GCGATAATTCAAGTGTTCAGAACAGAACATACATCGTCACAACAATCCTAGAAGATCCTTATGTGATGCT
  CAAGAAGAACGCCAATCAGTTTGAGGGCAATGACCGTTACGAGGGCTACTGTGTAGAGCTGGCGGCAGAG
  ATTGCCAAGCACGTGGGCTACTCCTACCGTCTGGAGATTGTCAGTGATGGAAAATACGGAGCCCGAGACC
  CTGACACGAAGGCCTGGAATGGCATGGTGGGAGAGCTGGTCTATGGAAGAGCAGATGTGGCTGTGGCTCC
  CTTAACTATCACTTTGGTCCGGGAAGAAGTTATAGATTTCTCCAAACCATTTATGAGTTTGGGGATCTCC
  ATCATGATTAAAAAACCACAGAAATCCAAGCCGGGTGTCTTCTCCTTCCTTGATCCTTTGGCTTATGAGA
  TTTGGATGTGCATTGTTTTTGCCTACATTGGAGTGAGTGTTGTCCTCTTCCTGGTCAGCCGCTTCAGTCC
  CTATGAATGGCACAGTGAAGAGTTTGAGGAAGGACGGGACCAGACAACCAGTGACCAGTCCAATGAGTTT
  GGGATATTCAACAGTTTGTGGTTCTCCCTGGGAGCCTTCATGCAGCAAGGATGTGACATTTCTCCCAGGT
  CCCTGTCTGGTCGCATCGTTGGTGGCGTCTGGTGGTTCTTCACCTTAATCATCATCTCCTCATATACAGC
  CAATCTGGCCGCCTTCCTGACCGTGGAGAGGATGGTGTCTCCCATTGAGAGTGCAGAGGACCTAGCGAAG
  CAGACAGAAATTGCCTACGGGACGCTGGAAGCAGGATCTACTAAGGAGTTCTTCAGGAGGTCTAAAATTG
  CTGTGTTTGAGAAGATGTGGACATACATGAAGTCAGCAGAGCCATCAGTTTTTGTGCGGACCACAGAGGA
  GGGGATGATTCGAGTGAGGAAATCCAAAGGCAAATATGCCTACCTCCTGGAGTCCACCATGAATGAGTAC
  ATTGAGCAGCGGAAACCCTGTGACACCATGAAGGTGGGAGGTAACTTGGATTCCAAAGGCTATGGCATTG
  CAACACCCAAGGGGTCTGCCCTGAGAGGTCCCGTAAACCTAGCGGTTTTGAAACTCAGTGAGCAAGGCGT
  CTTAGACAAGCTGAAAAGCAAATGGTGGTACGATAAAGGGGAATGTGGAAGCAAGGACTCCGGAAGTAAG
  GACAAGACAAGCGCTCTGAGCCTCAGCAATGTGGCAGGCGTGTTCTACATCCTGATCGGAGGACTTGGAC
  TAGCCATGCTGGTTGCCTTAATCGAGTTCTGCTACAAATCCCGTAGTGAATCCAAGCGGATGAAGGGTTT
  TTGTTTGATCCCACAGCAATCCATCAACGAAGCCATACGGACATCGACCCTCCCCCGCAACAGCGGGGCA
  GGAGCCAGCAGCGGCGGCAGTGGAGAGAATGGTCGGGTGGTCAGCCATGACTTCCCCAAGTCCATGCAAT
  CGATTCCTTGCATGAGCCACAGTTCAGGGATGCCCTTGGGAGCCACGGGATTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234917 representing NM_001258020
  Red=Cloning site Green=Tags(s)
  
  MLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQDALISIIDHYKWQKFVYIYDADRGLSVLQKVLDTA
  AEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIIKLEKNGIGYHYILANLGF
  MDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQWKNSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQ
  SLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDG
  IRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAE
  IAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGIS
  IMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEF
  GIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAK
  QTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEY
  IEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPVNLAVLKLSEQGVLDKLKSKWWYDKGECGSKDSGSK
  DKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGA
  GASSGGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258020
• ORF Size: 2433 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258020.1, NP_001244949.1
• RefSeq Size: 5804 bp
• RefSeq ORF: 2436 bp
• Locus ID: 2890
• Cytogenetics: 5q33.2
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction
• Gene Summary: 'Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'