MAP4K3 (NM_001270425) Human Tagged ORF Clone

CAT#: RG234983

MAP4K3 (GFP-tagged) - Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAP4K3
• Synonyms: GLK; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234983 representing NM_001270425
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGACTTCGAGCTGATTCAGCGCATCGGCA
  GCGGCACCTACGGCGACGTCTACAAGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAAT
  AAAATTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATTATGATGAAAGACTGTAAACAC
  CCAAATATTGTTGCTTATTTTGGAAGCTATCTCAGGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTG
  GAGGTGGTTCTTTACAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATATGTTAG
  CAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAAAAATGCACAGAGATATAAAGGGAGCT
  AACATTCTATTAACGGATAATGGTCATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTA
  CAATTGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAGTTGCAGCTGTTGAGAG
  GAAGGGGGGTTACAATCAACTCTGTGATCTCTGGGCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTT
  CAGCCTCCTATGTTTGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTCAGCCTC
  CTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCACTTTGTGAAAATGGCACTTACCAAAAA
  TCCGAAAAAAAGACCTACTGCTGAAAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCT
  TTGGCAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCATGATTTCGATGATGATG
  ATCCTGAGCCTCTTGTTGCTGTACCACATAGAATTCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAAC
  ACGCTCAGAGATAACCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCACATCAT
  GAACTTGATCTGCAACTGGAATATGGACAAGGACACCAAGGTGGTTACTTTTTAGGTGCAAACAAGAGTC
  TTCTCAAGTCTGTTGAAGAAGAATTGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGA
  TGATGATGAATCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCCACCAAAGCCTAAGTCT
  ATCTTCATACCACAGGAAATGCATTCTACTGAGGATGAAAATCAAGGAACAATCAAGAGATGTCCCATGT
  CAGGGAGCCCAGCAAAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCAGATTACCCCCACACAAACC
  TGTTGCCTTAGGAAATGGAATGAGCTCCTTCCAGTTAAATGGTGAACGAGATGGCTCATTATGTCAACAA
  CAGAATGAACATAGAGGCACAAACCTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATG
  GTCTTCCTCCAACACCTAAAGTGCATATGGGTGCATGTTTTTCAAAAGTTTTTAATGGGTGTCCCTTGAA
  AATTCACTGTGCATCATCATGGATAAACCCAGATACAAGAGATCAGTACTTGATATTTGGTGCCGAAGAA
  GGGATTTATACCCTCAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTCCCTCGAAGGTGTA
  CATGGTTGTATGTAATGAACAATTGCTTGCTATCAATATCTGGTAAAGCTTCTCAGCTTTATTCCCATAA
  TTTACCAGGGCTTTTTGATTATGCAAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCACACAAACTC
  CCTGACAGAATACTGCCAAGGAAATTTTCTGTATCAGCAAAAATCCCTGAAACCAAATGGTGCCAGAAGT
  GTTGTGTTGTAAGAAATCCTTACACGGGCCATAAATACCTATGTGGAGCACTTCAGACTAGCATTGTTCT
  ATTAGAATGGGTTGAACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCATGTCCA
  CTTAGAATGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCCTTTAGTTTGTGTTGGTGTCAGTAGAG
  GTAGAGACTTCAACCAAGTGGTTCGATTTGAGACGGTCAATCCAAATTCTACCTCTTCATGGTTTACAGA
  ATCAGATACCCCACAGACAAATGTTACTCATGTAACCCAACTGGAGAGAGATACCATCCTTGTATGCTTG
  GACTGTTGTATAAAAATAGTAAATCTCCAAGGAAGATTAAAATCTAGCAGGAAATTGTCATCAGAACTCA
  CCTTTGATTTCCAGATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACATGGAAT
  GCAAGGTAGAAGTTTTAGATCTAATGAGGTAACACAAGAAATTTCAGATAGCACAAGAATTTTCAGGCTG
  CTTGGATCTGACAGGGTCGTGGTTTTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATAGCAATTTGT
  ACATCCTGGCGGGTCATGAAAACAGTTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234983 representing NM_001270425
  Red=Cloning site Green=Tags(s)
  
  MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKH
  PNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGA
  NILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAEL
  QPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRS
  LAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHH
  ELDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPKS
  IFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQ
  QNEHRGTNLSRKEKKDVPKPISNGLPPTPKVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEE
  GIYTLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLPVAIPAHKL
  PDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCP
  LRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCL
  DCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRL
  LGSDRVVVLESRPTDNPTANSNLYILAGHENSY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270425
• ORF Size: 2619 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001270425.1, NP_001257354.1
• RefSeq Size: 4299
• RefSeq ORF: 2622
• Locus ID: 8491
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]