R Cadherin (CDH4) (NM_001252338) Human Tagged ORF Clone

CAT#: RG234988

CDH4 (GFP-tagged) - Homo sapiens cadherin 4, type 1, R-cadherin (retinal) (CDH4), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDH4
• Synonyms: CAD4; R-CAD; RCAD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234988 representing NM_001252338
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGCAGCTGTGACTTCATTCTCAGCGCCTCCTTTCTACCCTGCTTTAAAGAAGTCAAGTTCAGCA
  GCTGTGTGGGGACCAAGGGGACACAATATGAGACCAACAGCATGGACTTCAAAGTTGGGGCAGATGGGAC
  AGTCTTCGCCACCCGGGAGCTGCAGGTCCCCTCCGAGCAGGTGGCGTTCACGGTGACTGCATGGGACAGC
  CAGACAGCAGAGAAATGGGACGCCGTGGTGCGGTTGCTGGTGGCCCAGACCTCGTCCCCGCACTCTGGAC
  ACAAGCCGCAGAAAGGAAAGAAGGTCGTGGCTCTGGACCCCTCTCCGCCTCCGAAGGACACCCTGCTGCC
  GTGGCCCCAGCACCAGAACGCCAACGGGCTGAGGCGGCGCAAACGGGACTGGGTCATCCCGCCCATCAAC
  GTGCCCGAGAACTCGCGCGGGCCCTTCCCGCAGCAGCTCGTGAGGATCCGGTCCGACAAAGACAATGACA
  TCCCCATCCGGTACAGCATCACGGGAGTGGGCGCCGACCAGCCCCCCATGGAGGTCTTCAGCATTGACTC
  CATGTCCGGCCGGATGTACGTCACAAGGCCCATGGACCGGGAGGAGCACGCCTCTTACCACCTCCGAGCC
  CACGCTGTGGACATGAATGGCAACAAGGTGGAGAACCCCATCGACCTGTACATCTACGTCATCGACATGA
  ATGACAACCGCCCTGAGTTCATCAACCAGGTCTACAACGGCTCCGTGGACGAGGGCTCCAAGCCAGGCAC
  CTACGTGATGACCGTCACGGCCAACGATGCTGACGACAGCACCACGGCCAACGGGATGGTGCGGTACCGG
  ATCGTGACCCAGACCCCACAGAGCCCGTCCCAGAATATGTTCACCATCAACAGCGAGACTGGAGATATCG
  TCACAGTGGCGGCTGGCCTGGACCGAGAGAAAGTTCAGCAGTACACAGTCATCGTTCAGGCCACAGATAT
  GGAAGGAAATCTCAACTATGGCCTCTCAAACACAGCCACAGCCATCATCACGGTGACAGATGTGAATGAC
  AACCCGCCAGAATTTACCGCCAGCACGTTTGCAGGGGAGGTCCCCGAAAACCGCGTGGAGACCGTGGTCG
  CAAACCTCACGGTGATGGACCGAGATCAGCCCCACTCTCCAAACTGGAATGCCGTTTACCGCATCATCAG
  TGGGGATCCATCCGGGCACTTCAGCGTCCGCACAGACCCCGTAACCAACGAGGGCATGGTCACCGTGGTG
  AAGGCAGTCGACTACGAGCTCAACAGAGCTTTCATGCTGACAGTGATGGTGTCCAACCAGGCGCCCCTGG
  CCAGCGGAATCCAGATGTCCTTCCAGTCCACGGCAGGGGTGACCATCTCCATCATGGACATCAACGAGGC
  TCCCTACTTCCCCTCAAACCACAAGCTGATCCGCCTGGAGGAGGGCGTGCCCCCCGGCACCGTGCTGACC
  ACGTTTTCAGCTGTGGACCCTGACCGGTTCATGCAGCAGGCTGTGAGATACTCAAAGCTGTCAGACCCAG
  CGAGCTGGCTGCACATCAATGCCACCAACGGCCAGATCACCACGGCGGCAGTGCTGGACCGTGAGTCCCT
  CTACACCAAAAACAACGTCTACGAGGCCACCTTCCTGGCAGCTGACAATGGGATACCCCCGGCCAGCGGC
  ACCGGGACCCTCCAGATCTATCTCATTGACATCAACGACAACGCCCCTGAGCTGCTGCCCAAGGAGGCGC
  AGATCTGCGAGAAGCCCAACCTGAACGCCATCAACATCACGGCGGCCGACGCTGACGTCGACCCCAACAT
  CGGCCCCTACGTCTTCGAGCTGCCCTTTGTCCCGGCGGCCGTGCGGAAGAACTGGACCATCACCCGCCTG
  AACGGTGACTATGCCCAACTCAGCTTGCGCATCCTGTACCTGGAGGCCGGGATGTATGACGTCCCCATCA
  TCGTCACAGACTCTGGAAACCCTCCCCTGTCCAACACGTCCATCATCAAAGTCAAGGTGTGCCCATGTGA
  TGACAACGGGGACTGCACCACCATTGGCGCAGTGGCAGCGGCTGGTCTGGGCACCGGTGCCATCGTGGCC
  ATCCTCATCTGCATCCTCATCCTGCTGACCATGGTCCTGCTGTTTGTCATGTGGATGAAGCGGCGAGAGA
  AGGAGCGCCACACGAAGCAGCTGCTCATTGACCCCGAGGACGACGTCCGCGACAACATCCTCAAGTATGA
  CGAGGAAGGCGGTGGCGAGGAGGACCAGGACTACGACCTCAGCCAGCTGCAGCAGCCGGAAGCCATGGGG
  CACGTGCCAAGCAAAGCCCCTGGCGTGCGTCGCGTGGATGAGCGGCCGGTGGGCGCTGAGCCCCAGTACC
  CGATCAGGCCCATGGTGCCGCACCCAGGCGACATCGGTGACTTCATCAATGAGGGACTCCGCGCTGCTGA
  CAACGACCCCACGGCACCCCCCTATGACTCCCTGCTGGTCTTCGACTACGAGGGGAGCGGCTCCACCGCA
  GGCTCCGTCAGCTCCCTGAACTCATCCAGTTCCGGGGACCAAGACTACGATTACCTCAACGACTGGGGGC
  CCAGATTCAAGAAGCTGGCGGACATGTATGGAGGTGGTGAAGAGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234988 representing NM_001252338
  Red=Cloning site Green=Tags(s)
  
  MNGSCDFILSASFLPCFKEVKFSSCVGTKGTQYETNSMDFKVGADGTVFATRELQVPSEQVAFTVTAWDS
  QTAEKWDAVVRLLVAQTSSPHSGHKPQKGKKVVALDPSPPPKDTLLPWPQHQNANGLRRRKRDWVIPPIN
  VPENSRGPFPQQLVRIRSDKDNDIPIRYSITGVGADQPPMEVFSIDSMSGRMYVTRPMDREEHASYHLRA
  HAVDMNGNKVENPIDLYIYVIDMNDNRPEFINQVYNGSVDEGSKPGTYVMTVTANDADDSTTANGMVRYR
  IVTQTPQSPSQNMFTINSETGDIVTVAAGLDREKVQQYTVIVQATDMEGNLNYGLSNTATAIITVTDVND
  NPPEFTASTFAGEVPENRVETVVANLTVMDRDQPHSPNWNAVYRIISGDPSGHFSVRTDPVTNEGMVTVV
  KAVDYELNRAFMLTVMVSNQAPLASGIQMSFQSTAGVTISIMDINEAPYFPSNHKLIRLEEGVPPGTVLT
  TFSAVDPDRFMQQAVRYSKLSDPASWLHINATNGQITTAAVLDRESLYTKNNVYEATFLAADNGIPPASG
  TGTLQIYLIDINDNAPELLPKEAQICEKPNLNAINITAADADVDPNIGPYVFELPFVPAAVRKNWTITRL
  NGDYAQLSLRILYLEAGMYDVPIIVTDSGNPPLSNTSIIKVKVCPCDDNGDCTTIGAVAAAGLGTGAIVA
  ILICILILLTMVLLFVMWMKRREKERHTKQLLIDPEDDVRDNILKYDEEGGGEEDQDYDLSQLQQPEAMG
  HVPSKAPGVRRVDERPVGAEPQYPIRPMVPHPGDIGDFINEGLRAADNDPTAPPYDSLLVFDYEGSGSTA
  GSVSSLNSSSSGDQDYDYLNDWGPRFKKLADMYGGGEED
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252338
• ORF Size: 2637 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252338.1, NP_001239267.1
• RefSeq Size: 6343 bp
• RefSeq ORF: 2640 bp
• Locus ID: 1002
• Cytogenetics: 20q13.33
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: 'This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]'