LONP1 (NM_001276479) Human Tagged ORF Clone

CAT#: RG234998

LONP1 (GFP-tagged) - Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LONP1
• Synonyms: CODASS; hLON; LON; LonHS; LONP; PIM1; PRSS15
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234998 representing NM_001276479
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGAGCACTGGCTACGTGCGACTGTGGGGAGCGGCGCGGTGCTGGGTGCTGCGGCGGCCGATGC
  TGGCCGCCGCCGGGGGGCGGGTTCCCACTGCAGCAGGAGCGTGGTTGCTCCGAGGCCCGGTCATAACGGC
  GCTCACGCCCATGACGATCCCCGATGTGTTTCCGCACCTGCCGCTCATCGCCATCACCCGCAACCCGGTG
  TTCCCGCGCTTTATCAAGATTATCGAGGTTAAAAATAAGAAGTTGGTTGAGCTGCTGAGAAGGAAAGTTC
  GTCTCGCCCAGCCTTATGTCGGCGTCTTTCTAAAGAGAGATGACAGCAATGAGTCGGATGTGGTCGAGAG
  CCTGGATGAAATCTACCACACGGGGACGTTTGCCCAGATCCATGAGATGCAGGACCTTGGGGACAAGCTG
  CGCATGATCGTCATGGGACACAGAAGAGTCCATATCAGCAGACAGCTGGAGGTGGAGCCCGAGGAGCCGG
  AGGCGGAGAACAAGCACAAGCCCCGCAGGAAGTCAAAGCGGGGCAAGAAGGAGGCGGAGGACGAGCTGAG
  CGCCAGGCACCCGGCGGAGCTGGCGATGGAGCCCACCCCTGAGCTCCCGGCTGAGGTGCTCATGGTGGAG
  GTAGAGAACGTTGTCCACGAGGACTTCCAGGTCACGGAGGAGGTGAAAGCCCTGACTGCAGAGATCGTGA
  AGACCATCCGGGACATCATTGCCTTGAACCCTCTCTACAGGGAGTCAGTGCTGCAGATGATGCAGGCTGG
  CCAGCGGGTGGTGGACAACCCCATCTACCTGAGCGACATGGGCGCCGCGCTCACCGGGGCCGAGTCCCAT
  GAGCTGCAGGACGTCCTGGAAGAGACCAATATTCCTAAGCGGCTGTACAAGGCCCTCTCCCTGCTGAAGA
  AGGAATTTGAACTGAGCAAGCTGCAGCAGCGCCTGGGGCGGGAGGTGGAGGAGAAGATCAAGCAGACCCA
  CCGTAAGTACCTGCTGCAGGAGCAGCTAAAGATCATCAAGAAGGAGCTGGGCCTGGAGAAGGACGACAAG
  GATGCCATCGAGGAGAAGTTCCGGGAGCGCCTGAAGGAGCTCGTGGTCCCCAAGCACGTCATGGATGTTG
  TGGACGAGGAGCTGAGCAAGCTGGGCCTGCTGGACAACCACTCCTCGGAGTTCAATGTCACCCGCAACTA
  CCTAGACTGGCTCACGTCCATCCCTTGGGGCAAGTACAGCAACGAGAACCTGGACCTGGCGCGGGCACAG
  GCAGTGCTGGAGGAAGACCACTACGGCATGGAGGACGTCAAGAAACGCATCCTGGAGTTCATTGCCGTTA
  GCCAGCTCCGCGGCTCCACCCAGGGCAAGATCCTCTGCTTCTATGGCCCCCCTGGCGTGGGTAAGACCAG
  CATTGCTCGCTCCATCGCCCGCGCCCTGAACCGAGAGTACTTCCGCTTCAGCGTCGGGGGCATGACTGAC
  GTGGCTGAGATCAAGGGCCACAGGCGGACCTACGTGGGCGCCATGCCCGGGAAGATCATCCAGTGTTTGA
  AGAAGACCAAGACGGAGAACCCCCTGATCCTCATCGACGAGGTGGACAAGATCGGCCGAGGCTACCAGGG
  GGACCCGTCGTCGGCACTGCTGGAGCTGCTGGACCCAGAGCAGAATGCCAACTTCCTGGACCACTACCTG
  GACGTGCCCGTGGACTTGTCCAAGGTGCTGTTCATCTGCACGGCCAACGTCACGGACACCATCCCCGAGC
  CGCTGCGAGACCGTATGGAGATGATCAACGTGTCGGGCTACGTGGCCCAGGAGAAGCTGGCCATTGCGGA
  GCGCTACCTGGTGCCCCAGGCTCGCGCCCTGTGTGGCTTGGATGAGAGCAAGGCCAAGCTGTCATCGGAC
  GTGCTGACGCTGCTCATCAAGCAGTACTGCCGCGAGAGCGGTGTCCGCAACCTGCAGAAGCAAGTGGAGA
  AGGTGTTACGGAAATCGGCCTACAAGATTGTCAGCGGCGAGGCCGAGTCCGTGGAGGTGACGCCCGAGAA
  CCTGCAGGACTTCGTGGGGAAGCCCGTGTTCACCGTGGAGCGCATGTATGACGTGACACCGCCCGGCGTG
  GTCATGGGGCTGGCCTGGACCGCAATGGGAGGCTCCACGCTGTTTGTGGAGACATCCCTGAGACGGCCAC
  AGGACAAGGATGCCAAGGGTGACAAGGATGGCAGCCTGGAGGTGACAGGCCAGCTGGGGGAGGTGATGAA
  GGAGAGCGCCCGCATAGCCTACACCTTCGCCAGAGCCTTCCTCATGCAGCACGCCCCCGCCAATGACTAC
  CTGGTGACCTCACACATCCACCTGCATGTGCCCGAGGGCGCCACCCCCAAGGACGGCCCAAGCGCAGGCT
  GCACCATCGTCACGGCCCTGCTGTCCCTGGCCATGGGCAGGCCTGTCCGGCAGAATCTGGCCATGACTGG
  CGAAGTCTCCCTCACGGGCAAGATCCTGCCTGTTGGTGGCATCAAGGAGAAGACCATTGCGGCCAAGCGC
  GCAGGGGTGACGTGCATCGTCCTGCCAGCCGAGAACAAGAAGGACTTCTACGACCTGGCAGCCTTCATCA
  CCGAGGGCCTGGAGGTGCACTTCGTGGAACACTACCGGGAGATCTTCGACATCGCCTTCCCGGACGAGCA
  GGCAGAGGCGCTGGCCGTGGAACGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234998 representing NM_001276479
  Red=Cloning site Green=Tags(s)
  
  MAASTGYVRLWGAARCWVLRRPMLAAAGGRVPTAAGAWLLRGPVITALTPMTIPDVFPHLPLIAITRNPV
  FPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKL
  RMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVE
  VENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESH
  ELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDK
  DAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNENLDLARAQ
  AVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFRFSVGGMTD
  VAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYL
  DVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSD
  VLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGV
  VMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDY
  LVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKR
  AGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276479
• ORF Size: 2685 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001276479.1, NP_001263408.1
• RefSeq Size: 2936
• RefSeq ORF: 2688
• Locus ID: 9361
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome. Decreased expression of this gene has been noted in a patient with hereditary spastic paraplegia (PMID:18378094). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]