RTN3 (NM_001265590) Human Tagged ORF Clone

CAT#: RG235038

RTN3 (GFP-tagged) - Homo sapiens reticulon 3 (RTN3), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RTN3
• Synonyms: ASYIP; HAP; NSPL2; NSPLII; RTN3-A1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235038 representing NM_001265590
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCGTCGGCGGCCACTCAGTCCCATTCCATCTCCTCGTCGTCCTTCGGAGCCGAGCCGTCCG
  CGCCCGGCGGCGGCGGGAGCCCAGGAGCCTGCCCCGCCCTGGGGACGAAGAGCTGCAGCTCCTCCTGTGC
  GGCCAGTTTCCCAGAGCATCCTGCTTTTCTCTCAAAGAAAATTGGTCAAGTGGAAGAGCAAATAGATAAA
  GAGACCAAGAACCCAAATGGGGTATCAAGTAGGGAGGCTAAAACTGCATTGGATGCTGATGACAGATTCA
  CTTTGCTGACAGCCCAGAAACCACCTACTGAGTACTCTAAGGTAGAAGGCATTTATACATATTCTTTGTC
  TCCATCCAAAGTTTCAGGAGATGATGTTATTGAAAAGGATTCCCCTGAATCACCATTTGAAGTAATTATT
  GACAAAGCAGCATTTGACAAAGAATTTAAAGACTCATATAAGGAGAGCACAGATGATTTTGGTAGCTGGT
  CTGTGCACACTGATAAAGAATCATCCGAAGACATTTCAGAGACTAATGACAAGCTTTTTCCACTGAGAAA
  TAAAGAGGCAGGACGTTACCCAATGTCTGCATTGCTCAGTAGGCAGTTTTCACACACAAATGCAGCACTG
  GAAGAGGTGTCCAGATGCGTGAATGATATGCATAACTTTACTAACGAAATACTGACTTGGGATCTGGTTC
  CCCAAGTGAAACAACAGACCGATAAATCTTCTGACTGCATCACAAAAACTACAGGACTTGACATGAGTGA
  ATATAATTCAGAAATTCCAGTTGTAAATCTTAAAACTAGCACTCATCAGAAAACTCCTGTATGTTCTATT
  GATGGGAGCACTCCCATCACTAAATCAACAGGTGATTGGGCAGAAGCATCTCTCCAGCAAGAAAATGCTA
  TTACTGGAAAACCTGTACCTGACTCTTTGAATTCCACAAAAGAATTCAGTATCAAAGGTGTGCAAGGCAA
  TATGCAGAAACAGGATGACACACTTGCAGAATTACCTGGATCTCCACCTGAGAAATGTGACTCTTTGGGT
  TCTGGAGTGGCCACAGTGAAAGTGGTTTTACCTGATGACCACCTGAAAGATGAAATGGACTGGCAGAGCT
  CTGCATTGGGAGAAATCACAGAAGCTGATAGTTCTGGTGAGTCTGATGACACAGTAATAGAGGACATCAC
  AGCAGATACATCATTTGAAAATAACAAAATTCAGGCTGAAAAACCTGTTTCCATTCCAAGTGCTGTTGTA
  AAAACAGGTGAAAGAGAAATCAAAGAGATTCCCAGTTGTGAGAGAGAAGAAAAAACATCTAAAAACTTTG
  AAGAATTGGTCAGTGACTCTGAGCTGCATCAAGATCAGCCTGATATTCTTGGAAGGAGTCCAGCTAGTGA
  GGCAGCATGTTCAAAAGTACCCGATACGAATGTCTCCTTAGAAGATGTGAGTGAAGTTGCTCCTGAAAAG
  CCTATTACTACTGAGAACCCCAAACTTCCTTCAACAGTGTCTCCAAATGTTTTTAATGAGACAGAATTCT
  CATTAAATGTGACAACATCTGCCTATTTGGAGTCATTACATGGGAAAAATGTTAAACATATAGATGATTC
  CTCCCCAGAGGACCTGATAGCAGCCTTTACAGAAACCAGAGATAAAGGAATAGTAGATAGTGAAAGAAAT
  GCTTTTAAAGCAATATCAGAGAAGATGACAGACTTTAAAACAACTCCTCCTGTAGAAGTCTTACATGAAA
  ATGAGTCCGGTGGTTCTGAAATTAAAGACATTGGAAGCAAATACAGTGAACAAAGCAAAGAAACAAATGG
  AAGTGAGCCTCTAGGTGTTTTCCCTACCCAAGGTACTCCAGTAGCATCTCTTGACTTAGAACAAGAACAG
  CTCACAATTAAGGCTCTTAAAGAATTAGGTGAAAGACAGGTTGAGAAGTCAACTTCTGCACAGCGTGACG
  CAGAATTGCCTTCTGAAGAAGTACTGAAGCAAACTTTCACATTTGCTCCAGAATCTTGGCCACAGAGATC
  ATATGACATCCTAGAACGTAATGTCAAGAATGGATCTGATCTTGGGATTTCCCAGAAGCCCATCACTATC
  AGAGAAACTACTAGGGTAGATGCTGTTTCCAGCCTTAGCAAGACTGAATTGGTAAAAAAGCATGTCCTAG
  CAAGACTTCTGACAGACTTCTCAGTGCACGATCTGATTTTCTGGAGAGATGTGAAGAAGACTGGGTTTGT
  CTTTGGCACCACGCTGATCATGCTGCTTTCCCTGGCAGCTTTCAGTGTCATCAGTGTGGTTTCTTACCTC
  ATCCTGGCTCTTCTCTCTGTCACCATCAGCTTCAGGATCTACAAGTCCGTCATCCAAGCTGTACAGAAGT
  CAGAAGAAGGCCATCCATTCAAAGCCTACCTGGACGTAGACATTACTCTGTCCTCAGAAGCTTTCCATAA
  TTACATGAATGCTGCCATGGTGCACATCAACAGGGCCCTGAAACTCATTATTCGTCTCTTTCTGGTAGAA
  GATCTGGTTGACTCCTTGAAGCTGGCTGTCTTCATGTGGCTGATGACCTATGTTGGTGCTGTTTTTAACG
  GAATCACCCTTCTAATTCTTGCTGAACTGCTCATTTTCAGTGTCCCGATTGTCTATGAGAAGTACAAGAC
  CCAGATTGATCACTATGTTGGCATCGCCCGAGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAA
  CTCCCTGGAATCGCCAAAAAAAAGGCAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235038 representing NM_001265590
  Red=Cloning site Green=Tags(s)
  
  MAEPSAATQSHSISSSSFGAEPSAPGGGGSPGACPALGTKSCSSSCAASFPEHPAFLSKKIGQVEEQIDK
  ETKNPNGVSSREAKTALDADDRFTLLTAQKPPTEYSKVEGIYTYSLSPSKVSGDDVIEKDSPESPFEVII
  DKAAFDKEFKDSYKESTDDFGSWSVHTDKESSEDISETNDKLFPLRNKEAGRYPMSALLSRQFSHTNAAL
  EEVSRCVNDMHNFTNEILTWDLVPQVKQQTDKSSDCITKTTGLDMSEYNSEIPVVNLKTSTHQKTPVCSI
  DGSTPITKSTGDWAEASLQQENAITGKPVPDSLNSTKEFSIKGVQGNMQKQDDTLAELPGSPPEKCDSLG
  SGVATVKVVLPDDHLKDEMDWQSSALGEITEADSSGESDDTVIEDITADTSFENNKIQAEKPVSIPSAVV
  KTGEREIKEIPSCEREEKTSKNFEELVSDSELHQDQPDILGRSPASEAACSKVPDTNVSLEDVSEVAPEK
  PITTENPKLPSTVSPNVFNETEFSLNVTTSAYLESLHGKNVKHIDDSSPEDLIAAFTETRDKGIVDSERN
  AFKAISEKMTDFKTTPPVEVLHENESGGSEIKDIGSKYSEQSKETNGSEPLGVFPTQGTPVASLDLEQEQ
  LTIKALKELGERQVEKSTSAQRDAELPSEEVLKQTFTFAPESWPQRSYDILERNVKNGSDLGISQKPITI
  RETTRVDAVSSLSKTELVKKHVLARLLTDFSVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSYL
  ILALLSVTISFRIYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMVHINRALKLIIRLFLVE
  DLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLIFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKIQAK
  LPGIAKKKAE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001265590
• ORF Size: 2760 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001265590.1, NP_001252519.1
• RefSeq Size: 4640
• RefSeq ORF: 2763
• Locus ID: 10313
• Protein Families: Transmembrane
• Gene Summary: This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. An increase in the expression of any reticulon protein substantially reduces the production of amyloid-beta, suggesting that reticulon proteins are negative modulators of BACE1 in cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, and pseudogenes of this gene are located on chromosomes 4 and 12. [provided by RefSeq, May 2012]