Zonula occludens protein 3 (TJP3) (NM_001267561) Human Tagged ORF Clone

CAT#: RG235043

TJP3 (GFP-tagged) - Homo sapiens tight junction protein 3 (TJP3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TJP3
• Synonyms: ZO-3; ZO3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235043 representing NM_001267561
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGAGATTCCAGGTGGCTGACATGGAGGAGCTGACCATCTGGGAACAGCACACGGCCACACTGT
  CCAAGGACCCCCGCCGGGGCTTTGGCATTGCGATCTCTGGAGGCCGAGACCGGCCCGGTGGATCCATGGT
  TGTATCTGACGTGGTACCTGGAGGGCCGGCGGAGGGCAGGCTACAGACAGGCGACCACATCGTCATGGTG
  AACGGGGTTTCCATGGAGAATGCCACCTCCGCGTTTGCCATTCAGATACTCAAGACCTGCACCAAGATGG
  CCAACATCACAGTGAAACGTCCCCGGAGGATCCACCTGCCCGCCACCAAAGCCAGCCCCTCCAGCCCAGG
  GCGCCAGGACTCGGATGAAGACGATGGGCCCCAGCGGGTGGAGGAGGTGGACCAGGGCCGGGGCTATGAC
  GGCGACTCATCCAGTGGCTCCGGCCGCTCCTGGGACGAGCGCTCCCGCCGGCCGAGGCCTGGTCGCCGGG
  GCCGGGCCGGCAGCCATGGGCGTAGGAGCCCAGGTGGTGGCTCTGAGGCCAACGGGCTGGCCCTGGTGTC
  CGGCTTTAAGCGGCTGCCACGGCAGGACGTGCAGATGAAGCCTGTGAAGTCAGTGCTGGTGAAGAGGAGA
  GACAGCGAAGAGTTTGGCGTCAAGCTGGGCAGTCAGATCTTCATCAAGCACATTACAGATTCGGGCCTGG
  CTGCCCGGCACCGTGGGCTGCAGGAAGGAGATCTCATTCTACAGATCAACGGGGTGTCTAGCCAGAACCT
  GTCACTGAACGACACCCGGCGACTGATTGAGAAGTCAGAAGGGAAGCTAAGCCTGCTGGTGCTGAGAGAT
  CGTGGGCAGTTCCTGGTGAACATTCCGCCTGCTGTCAGTGACAGCGACAGCTCGCCATTGGAGGACATCT
  CGGACCTCGCCTCGGAGCTATCGCAGGCACCACCATCCCACATCCCACCACCACCCCGGCATGCTCAGCG
  GAGCCCCGAGGCCAGCCAGACCGACTCTCCCGTGGAGAGTCCCCGGCTTCGGCGGGAAAGTTCAGTAGAT
  TCCAGAACCATCTCGGAACCAGATGAGCAACGGTCAGAGTTGCCCAGGGAAAGCAGCTATGACATCTACA
  GAGTGCCCAGCAGTCAGAGCATGGAGGATCGTGGGTACAGCCCCGACACGCGTGTGGTCCGCTTCCTCAA
  GGGCAAGAGCATCGGGCTGCGGCTGGCAGGGGGCAATGACGTGGGCATCTTCGTGTCCGGGGTGCAGGCG
  GGCAGCCCGGCCGACGGGCAGGGCATCCAGGAGGGAGATCAGATTCTGCAGGTGAATGACGTGCCATTCC
  AGAACCTGACACGGGAGGAGGCAGTGCAGTTCCTGCTGGGGCTGCCACCAGGCGAGGAGATGGAGCTGGT
  GACGCAGAGGAAGCAGGACATTTTCTGGAAAATGGTGCAGTCCCGCGTGGGTGACTCCTTCTACATCCGC
  ACTCACTTTGAGCTGGAGCCCAGTCCACCGTCTGGCCTGGGCTTCACCCGTGGCGACGTCTTCCACGTGC
  TGGACACGCTGCACCCCGGCCCCGGGCAGAGCCACGCACGAGGAGGCCACTGGCTGGCGGTGCGCATGGG
  TCGTGACCTGCGGGAGCAAGAGCGGGGCATCATTCCCAACCAGAGCAGGGCGGAGCAGCTGGCCAGCCTG
  GAAGCTGCCCAGAGGGCCGTGGGAGTCGGGCCCGGCTCCTCCGCGGGCTCCAATGCTCGGGCCGAGTTCT
  GGCGGCTGCGGGGTCTTCGTCGAGGAGCCAAGAAGACCACTCAGCGGAGCCGTGAGGACCTCTCAGCTCT
  GACCCGACAGGGCCGCTACCCGCCCTACGAACGAGTGGTGTTGCGAGAAGCCAGTTTCAAGCGCCCGGTA
  GTGATCCTGGGACCCGTGGCCGACATTGCTATGCAGAAGTTGACTGCTGAGATGCCTGACCAGTTTGAAA
  TCGCAGAGACTGTGTCCAGGACCGACAGCCCCTCCAAGATCATCAAACTAGACACCGTGCGGGTGATTGC
  AGAAAAAGACAAGCATGCGCTCCTGGATGTGACCCCCTCCGCCATCGAGCGCCTCAACTATGTGCAGTAC
  TACCCCATTGTGGTCTTCTTCATCCCCGAGAGCCGGCCGGCCCTCAAGGCACTGCGCCAGTGGCTGGCGC
  CTGCCTCCCGCCGCAGCACCCGTCGCCTCTACGCACAAGCCCAGAAGCTGCGAAAACACAGCAGCCACCT
  CTTCACAGCCACCATCCCTCTGAATGGCACGAGTGACACCTGGTACCAGGAGCTCAAGGCCATCATTCGA
  GAGCAGCAGACGCGGCCCATCTGGACGGCGGAAGATCAGCTGGATGGCTCCTTGGAGGACAACCTAGACC
  TCCCTCACCACGGCCTGGCCGACAGCTCCGCTGACCTCAGCTGCGACAGCCGCGTTAACAGCGACTACGA
  GACGGACGGCGAGGGCGGCGCGTACACGGATGGCGAGGGCTACACAGACGGCGAGGGGGGGCCCTACACG
  GATGTGGATGATGAGCCCCCGGCTCCAGCCCTGGCCCGGTCCTCGGAGCCCGTGCAGGCAGATGAGTCCC
  AGAGCCCGAGGGATCGTGGGAGAATCTCGGCTCATCAGGGGGCCCAGGTGGACAGCCGCCACCCCCAGGG
  ACAGTGGCGACAGGACAGCATGCGAACCTATGAACGGGAAGCCCTGAAGAAAAAGTTTATGCGAGTACAT
  GATGCGGAGTCCTCCGATGAAGACGGCTATGACTGGGGTCCGGCCACTGACCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235043 representing NM_001267561
  Red=Cloning site Green=Tags(s)
  
  MAVRFQVADMEELTIWEQHTATLSKDPRRGFGIAISGGRDRPGGSMVVSDVVPGGPAEGRLQTGDHIVMV
  NGVSMENATSAFAIQILKTCTKMANITVKRPRRIHLPATKASPSSPGRQDSDEDDGPQRVEEVDQGRGYD
  GDSSSGSGRSWDERSRRPRPGRRGRAGSHGRRSPGGGSEANGLALVSGFKRLPRQDVQMKPVKSVLVKRR
  DSEEFGVKLGSQIFIKHITDSGLAARHRGLQEGDLILQINGVSSQNLSLNDTRRLIEKSEGKLSLLVLRD
  RGQFLVNIPPAVSDSDSSPLEDISDLASELSQAPPSHIPPPPRHAQRSPEASQTDSPVESPRLRRESSVD
  SRTISEPDEQRSELPRESSYDIYRVPSSQSMEDRGYSPDTRVVRFLKGKSIGLRLAGGNDVGIFVSGVQA
  GSPADGQGIQEGDQILQVNDVPFQNLTREEAVQFLLGLPPGEEMELVTQRKQDIFWKMVQSRVGDSFYIR
  THFELEPSPPSGLGFTRGDVFHVLDTLHPGPGQSHARGGHWLAVRMGRDLREQERGIIPNQSRAEQLASL
  EAAQRAVGVGPGSSAGSNARAEFWRLRGLRRGAKKTTQRSREDLSALTRQGRYPPYERVVLREASFKRPV
  VILGPVADIAMQKLTAEMPDQFEIAETVSRTDSPSKIIKLDTVRVIAEKDKHALLDVTPSAIERLNYVQY
  YPIVVFFIPESRPALKALRQWLAPASRRSTRRLYAQAQKLRKHSSHLFTATIPLNGTSDTWYQELKAIIR
  EQQTRPIWTAEDQLDGSLEDNLDLPHHGLADSSADLSCDSRVNSDYETDGEGGAYTDGEGYTDGEGGPYT
  DVDDEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVH
  DAESSDEDGYDWGPATDL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001267561
• ORF Size: 2784 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001267561.1, NP_001254490.1
• RefSeq Size: 3127
• RefSeq ORF: 2787
• Locus ID: 27134
• Protein Pathways: Tight junction
• Gene Summary: The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012]