BRDT (NM_001242805) Human Tagged ORF Clone
CAT#: RG235071
- TrueORF®
BRDT (GFP-tagged) - Homo sapiens bromodomain, testis-specific (BRDT), transcript variant 3
"NM_001242805" in other vectors (2)
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Other products for "BRDT"
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | BRDT |
Synonyms | BRD6; CT9; SPGF21 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG235071 representing NM_001242805
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGCCAAGTCGACAAACAGCTATTATTGTTAACCCTCCTCCACCAGAATATATAAATACTAAGA AAAATGGGCGATTGACAAATCAACTTCAGTATCTACAAAAAGTTGTCCTAAAGGATTTATGGAAGCATAG TTTTTCATGGCCCTTTCAACGTCCTGTGGATGCTGTGAAACTACAGTTGCCTGATTATTATACCATTATA AAAAACCCAATGGATTTAAATACAATTAAGAAGCGCTTGGAGAATAAATATTATGCGAAGGCTTCAGAAT GTATAGAAGACTTCAATACAATGTTCTCAAATTGTTATTTATATAACAAGCCTGGAGATGACATTGTTCT TATGGCACAAGCTCTAGAGAAGCTGTTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAAGTTGTG GGTGTTAAGGAAAGAATCAAGAAAGGCACTCAACAGAATATAGCTGTTTCTTCTGCTAAAGAAAAATCAT CACCCAGCGCAACAGAAAAAGTATTTAAGCAGCAAGAAATTCCTTCTGTATTTCCTAAGACATCTATTTC TCCCTTGAACGTGGTACAGGGAGCTTCAGTCAACTCCAGTTCACAAACTGCGGCCCAAGTTACAAAAGGT GTGAAGAGGAAAGCAGATACAACAACTCCTGCAACTTCAGCAGTTAAAGCAAGTAGTGAATTTTCTCCAA CATTCACAGAAAAATCAGTGGCACTGCCACCTATAAAAGAAAATATGCCAAAGAATGTTTTGCCAGATTC TCAGCAACAATATAATGTTGTGAAGACTGTTAAAGTAACTGAACAATTAAGGCACTGTAGTGAGATTCTT AAAGAAATGCTTGCAAAGAAACATTTTTCATATGCATGGCCCTTTTATAATCCTGTTGACGTTAATGCTT TGGGACTCCATAACTACTATGACGTTGTCAAAAATCCGATGGATCTTGGAACTATTAAGGAGAAAATGGA TAACCAAGAATATAAGGATGCATACAAATTTGCGGCAGATGTTAGATTAATGTTCATGAATTGCTACAAG TACAATCCTCCAGATCACGAAGTTGTGACAATGGCAAGAATGCTTCAGGATGTTTTCGAAACGCATTTTT CAAAGATCCCGATTGAACCTGTTGAGAGTATGCCTTTATGTTACATCAAAACAGATATCACAGAAACCAC TGGTAGAGAGAACACTAATGAAGCCTCCTCTGAAGGGAACTCTTCTGATGATTCTGAAGATGAGCGAGTT AAGCGTCTTGCAAAGCTTCAGGAGCAGCTTAAAGCTGTACATCAACAGCTCCAGGTTTTGTCCCAAGTAC CTTTCCGTAAGCTAAATAAAAAGAAAGAGAAGTCTAAAAAGGAAAAGAAAAAAGAAAAGGTTAATAACAG CAATGAAAATCCAAGAAAAATGTGTGAGCAAATGAGGCTAAAGGAAAAGTCCAAGAGAAATCAGCCAAAG AAAAGGAAACAACAGTTCATTGGTCTAAAATCTGAAGATGAAGATAATGCTAAACCTATGAACTATGATG AGAAAAGGCAGTTAAGTCTGAATATAAACAAACTCCCTGGAGATAAACTTGGGCGAGTAGTTCACATAAT ACAATCAAGAGAGCCTTCTCTGAGCAATTCCAATCCTGATGAGATAGAGATAGACTTTGAAACACTGAAA GCATCAACACTAAGAGAATTAGAAAAATATGTTTCGGCATGTCTAAGAAAGAGACCATTAAAACCTCCTG CTAAGAAAATAATGATGTCCAAAGAAGAACTTCACTCACAGAAAAAACAGGAATTGGAAAAGCGGTTACT GGATGTTAATAATCAGTTAAATTCTAGAAAACGTCAAACAAAATCTGATAAAACGCAACCATCCAAAGCT GTTGAAAATGTTTCCCGACTGAGTGAGAGCAGCAGCAGCAGCAGCAGCTCATCAGAGTCTGAAAGTAGCA GCAGTGACTTAAGCTCTTCAGACAGCAGTGATTCTGAATCAGAAATGTTCCCTAAGTTTACAGAAGTAAA ACCAAATGATTCTCCTTCTAAAGAGAATGTAAAGAAAATGAAGAATGAATGCATACTGCCTGAAGGAAGA ACAGGCGTCACACAGATAGGATATTGTGTGCAAGACACAACCTCTGCCAATACTACCCTTGTTCATCAGA CCACACCTTCACATGTAATGCCACCAAATCACCACCAATTAGCATTTAATTATCAAGAATTAGAACATTT ACAGACTGTGAAAAACATTTCACCTTTACAAATTCTGCCTCCCTCAGGTGATTCTGAACAGCTCTCAAAT GGCATAACTGTGATGCATCCATCTGGTGATAGTGACACAACGATGTTAGAATCTGAATGTCAAGCTCCTG TACAGAAGGATATAAAGATTAAGAATGCAGATTCATGGAAAAGTTTAGGCAAACCAGTGAAACCATCAGG TGTAATGAAATCCTCAGATGAGCTCTTCAACCAATTTAGAAAAGCAGCCATAGAAAAGGAAGTAAAAGCT CGGACACAGGAACTCATACGGAAGCATTTGGAACAAAATACAAAGGAACTAAAAGCATCTCAAGAAAATC AGAGGGATCTTGGGAATGGATTGACTGTAGAATCTTTTTCAAATAAAATACAAAACAAGTGCTCTGGAGA AGAGCAGAAAGAACATCAGCAGTCATCAGAAGCTCAAGATAAATCCAAACTCTGGCTTCTCAAAGACCGT GATTTAGCAAGGCAGAAAGAACAAGAGAGGAGGAGGAGAGAAGCAATGGTGGGTACCATTGATATGACCC TTCAAAGTGACATTATGACAATGTTTGAAAACAACTTTGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235071 representing NM_001242805
Red=Cloning site Green=Tags(s) MSLPSRQTAIIVNPPPPEYINTKKNGRLTNQLQYLQKVVLKDLWKHSFSWPFQRPVDAVKLQLPDYYTII KNPMDLNTIKKRLENKYYAKASECIEDFNTMFSNCYLYNKPGDDIVLMAQALEKLFMQKLSQMPQEEQVV GVKERIKKGTQQNIAVSSAKEKSSPSATEKVFKQQEIPSVFPKTSISPLNVVQGASVNSSSQTAAQVTKG VKRKADTTTPATSAVKASSEFSPTFTEKSVALPPIKENMPKNVLPDSQQQYNVVKTVKVTEQLRHCSEIL KEMLAKKHFSYAWPFYNPVDVNALGLHNYYDVVKNPMDLGTIKEKMDNQEYKDAYKFAADVRLMFMNCYK YNPPDHEVVTMARMLQDVFETHFSKIPIEPVESMPLCYIKTDITETTGRENTNEASSEGNSSDDSEDERV KRLAKLQEQLKAVHQQLQVLSQVPFRKLNKKKEKSKKEKKKEKVNNSNENPRKMCEQMRLKEKSKRNQPK KRKQQFIGLKSEDEDNAKPMNYDEKRQLSLNINKLPGDKLGRVVHIIQSREPSLSNSNPDEIEIDFETLK ASTLRELEKYVSACLRKRPLKPPAKKIMMSKEELHSQKKQELEKRLLDVNNQLNSRKRQTKSDKTQPSKA VENVSRLSESSSSSSSSSESESSSSDLSSSDSSDSESEMFPKFTEVKPNDSPSKENVKKMKNECILPEGR TGVTQIGYCVQDTTSANTTLVHQTTPSHVMPPNHHQLAFNYQELEHLQTVKNISPLQILPPSGDSEQLSN GITVMHPSGDSDTTMLESECQAPVQKDIKIKNADSWKSLGKPVKPSGVMKSSDELFNQFRKAAIEKEVKA RTQELIRKHLEQNTKELKASQENQRDLGNGLTVESFSNKIQNKCSGEEQKEHQQSSEAQDKSKLWLLKDR DLARQKEQERRRREAMVGTIDMTLQSDIMTMFENNFD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001242805 |
ORF Size | 2841 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001242805.1, NP_001229734.1 |
RefSeq Size | 3430 bp |
RefSeq ORF | 2844 bp |
Locus ID | 676 |
Cytogenetics | 1p22.1 |
Protein Families | Protein Kinase, Transcription Factors |
Gene Summary | 'BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jun 2011]' |
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