REST (NM_001193508) Human Tagged ORF Clone
CAT#: RG235166
- TrueORF®
REST (GFP-tagged) - Homo sapiens RE1-silencing transcription factor (REST), transcript variant 2
"NM_001193508" in other vectors (2)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Other products for "REST"
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | REST |
Synonyms | DFNA27; GINGF5; HGF5; NRSF; WT6; XBR |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG235166 representing NM_001193508
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCCAGGTAATGGGGCAGTCTTCTGGAGGAGGAGGGCTGTTTACCAGCAGTGGCAACATTGGAA TGGCCCTGCCTAACGACATGTATGACTTGCATGACCTTTCCAAAGCTGAACTGGCCGCACCTCAGCTTAT TATGCTGGCAAATGTGGCCTTAACTGGGGAAGTAAATGGCAGCTGCTGTGATTACCTGGTCGGTGAAGAA AGACAGATGGCAGAACTGATGCCGGTTGGGGATAACAACTTTTCAGATAGTGAAGAAGGAGAAGGACTTG AAGAGTCTGCTGATATAAAAGGTGAACCTCATGGACTGGAAAACATGGAACTGAGAAGTTTGGAACTCAG CGTCGTAGAACCTCAGCCTGTATTTGAGGCATCAGGTGCTCCAGATATTTACAGTTCAAATAAAGATCTT CCCCCTGAAACACCTGGAGCGGAGGACAAAGGCAAGAGCTCGAAGACCAAACCCTTTCGCTGTAAGCCAT GCCAATATGAAGCAGAATCTGAAGAACAGTTTGTGCATCACATCAGAGTTCACAGTGCTAAGAAATTTTT TGTGGAAGAGAGTGCAGAGAAGCAGGCAAAAGCCAGGGAATCTGGCTCTTCCACTGCAGAAGAGGGAGAT TTCTCCAAGGGCCCCATTCGCTGTGACCGCTGCGGCTACAATACTAATCGATATGATCACTATACAGCAC ACCTGAAACACCACACCAGAGCTGGGGATAATGAGCGAGTCTACAAGTGTATCATTTGCACATACACAAC AGTGAGCGAGTATCACTGGAGGAAACATTTAAGAAACCATTTTCCAAGGAAAGTATACACATGTGGAAAA TGCAACTATTTTTCAGACAGAAAAAACAATTATGTTCAGCATGTTAGAACTCATACAGGAGAACGCCCAT ATAAATGTGAACTTTGTCCTTACTCAAGTTCTCAGAAGACTCATCTAACTAGACATATGCGTACTCATTC AGGTGAGAAGCCATTTAAATGTGATCAGTGCAGTTATGTGGCCTCTAATCAACATGAAGTAACCCGCCAT GCAAGACAGGTTCACAATGGGCCTAAACCTCTTAATTGCCCACACTGTGATTACAAAACAGCAGATAGAA GCAACTTCAAAAAACATGTAGAGCTACATGTGAACCCACGGCAGTTCAATTGCCCTGTATGTGACTATGC AGCTTCCAAGAAGTGTAATCTACAGTATCACTTCAAATCTAAGCATCCTACTTGTCCTAATAAAACAATG GATGTCTCAAAAGTGAAACTAAAGAAAACCAAAAAACGAGAGGCTGACTTGCCTGATAATATTACCAATG AAAAAACAGAAATAGAACAAACAAAAATAAAAGGGGATGTGGCTGGAAAGAAAAATGAAAAGTCCGTCAA AGCAGAGAAAAGAGATGTCTCAAAAGAGAAAAAGCCTTCTAATAATGTGTCAGTGATCCAGGTGACTACC AGAACTCGAAAATCAGTAACAGAGGTGAAAGAGATGGATGTGCATACAGGAAGCAATTCAGAAAAATTCA GTAAAACTAAGAAAAGCAAAAGGAAGCTGGAAGTTGACAGCCATTCTTTACATGGTCCTGTGAATGATGA GGAATCTTCAACAAAAAAGAAAAAGAAGGTAGAAAGCAAATCCAAAAATAATAGTCAGGAAGTGCCAAAG GGTGACAGCAAAGTGGAGGAGAATAAAAAGCAAAATACTTGCATGAAAAAAAGTACAAAGAAGAAAACTC TGAAAAATAAATCAAGTAAGAAAAGCAGTAAGCCTCCTCAGAAGGAACCTGTTGAGAAGGGATCTGCTCA GATGGACCCTCCTCAGATGGGGCCTGCTCCCACAGAGGCGGTTCAGAAGGGGCCCGTTCAGGTGGAGCCG CCACCTCCCATGGAGCATGCTCAGATGGAGGGTGCCCAGATACGGCCTGCTCCTGACGAGCCTGTTCAGA TGGAGGTGGTTCAGGAGGGGCCTGCTCAGAAGGAGCTGCTGCCTCCCGTGGAGCCTGCTCAGATGGTGGG TGCCCAAATTGTACTTGCTCACATGGAGCTGCCTCCTCCCATGGAGACTGCTCAGACGGAGGTTGCCCAA ATGGGGCCTGCTCCCATGGAACCTGCTCAGATGGAGGTTGCCCAGGTAGAATCTGCTCCCATGCAGGTGG TCCAGAAGGAGCCTGTTCAGATGGAGCTGTCTCCTCCCATGGAGGTGGTCCAGAAGGAGCCTGTTCAGAT AGAGCTGTCTCCTCCCATGGAGGTGGTCCAGAAGGAACCTGTTAAGATAGAGCTGTCTCCTCCCATAGAG GTGGTCCAGAAGGAGCCTGTTCAGATGGAGTTGTCTCCTCCCATGGGGGTGGTTCAGAAGGAGCCTGCTC AGAGGGAGCCACCTCCTCCCAGAGAGCCTCCCCTTCACATGGAGCCAATTTCCAAAAAGCCTCCTCTCCG AAAAGATAAAAAGGAAAAGTCTAACATGCAGAGTGAAAGGGCACGGAAGGAGCAAGTCCTTATTGAAGTT GGCTTAGTGCCTGTTAAAGATAGCTGGCTTCTAAAGGAAAGTGTAAGCACAGAGGATCTCTCACCACCAT CACCACCACTGCCAAAGGAAAATTTAAGAGAAGAGGCATCAGGAGACCAAAAATTACTCAACACAGGTGA AGGAAATAAAGAAGCCCCTCTTCAGAAAGTAGGAGCAGAAGAGGCAGATGAGAGCCTACCTGGTCTTGCT GCTAATATCAACGAATCTACCCATATTTCATCCTCTGGACAAAACTTGAATACGCCAGAGGGTGAAACTT TAAATGGTAAACATCAGACTGACAGTATAGTTTGTGAAATGAAAATGGACACTGATCAGAACACAAGAGA GAATCTCACTGGTATAAATTCAACAGTTGAAGAACCAGTTTCACCAATGCTTCCCCCTTCAGCAGTAGAA GAACGTGAAGCAGTGTCCAAAACTGCACTGGCATCACCTCCTGCTACAATGGCAGCAAATGAGTCTCAGG AAATTGATGAAGATGAAGGCATCCACAGCCATGAAGGAAGTGACCTAAGTGACAACATGTCAGAGGGTAG TGATGATTCTGGATTGCATGGGGCTCGGCCAGTTCCACAAGAATCTAGCAGAAAAAATGCAAAGGAAGCC TTGGCAGTCAAAGCGGCTAAGGGAGATTTTGTTTGTATCTTCTGTGATCGTTCTTTCAGAAAGGGAAAAG ATTACAGCAAACACCTCAATCGCCATTTGGTTAATGTGTACTATCTTGAAGAAGCAGCTCAAGGGCAGGA G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235166 representing NM_001193508
Red=Cloning site Green=Tags(s) MATQVMGQSSGGGGLFTSSGNIGMALPNDMYDLHDLSKAELAAPQLIMLANVALTGEVNGSCCDYLVGEE RQMAELMPVGDNNFSDSEEGEGLEESADIKGEPHGLENMELRSLELSVVEPQPVFEASGAPDIYSSNKDL PPETPGAEDKGKSSKTKPFRCKPCQYEAESEEQFVHHIRVHSAKKFFVEESAEKQAKARESGSSTAEEGD FSKGPIRCDRCGYNTNRYDHYTAHLKHHTRAGDNERVYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCGK CNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCSYVASNQHEVTRH ARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPNKTM DVSKVKLKKTKKREADLPDNITNEKTEIEQTKIKGDVAGKKNEKSVKAEKRDVSKEKKPSNNVSVIQVTT RTRKSVTEVKEMDVHTGSNSEKFSKTKKSKRKLEVDSHSLHGPVNDEESSTKKKKKVESKSKNNSQEVPK GDSKVEENKKQNTCMKKSTKKKTLKNKSSKKSSKPPQKEPVEKGSAQMDPPQMGPAPTEAVQKGPVQVEP PPPMEHAQMEGAQIRPAPDEPVQMEVVQEGPAQKELLPPVEPAQMVGAQIVLAHMELPPPMETAQTEVAQ MGPAPMEPAQMEVAQVESAPMQVVQKEPVQMELSPPMEVVQKEPVQIELSPPMEVVQKEPVKIELSPPIE VVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEPISKKPPLRKDKKEKSNMQSERARKEQVLIEV GLVPVKDSWLLKESVSTEDLSPPSPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLA ANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVE EREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEA LAVKAAKGDFVCIFCDRSFRKGKDYSKHLNRHLVNVYYLEEAAQGQE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001193508 |
ORF Size | 3291 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001193508.1, NP_001180437.1 |
RefSeq Size | 7122 bp |
RefSeq ORF | 3294 bp |
Locus ID | 5978 |
Cytogenetics | 4q12 |
Protein Families | Transcription Factors |
Protein Pathways | Huntington's disease |
Gene Summary | 'This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018]' |
Documents
Product Manuals |
FAQs |
Resources
{0} Product Review(s)
0 Product Review(s)
Submit review
Be the first one to submit a review
Product Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.