DIAPH3 (NM_001258368) Human Tagged ORF Clone

CAT#: RG235176

DIAPH3 (GFP-tagged) - Homo sapiens diaphanous-related formin 3 (DIAPH3), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DIAPH3
• Synonyms: AN; AUNA1; DIA2; diap3; DRF3; mDia2; NSDAN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235176 representing NM_001258368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACGGCACCAGCCGCGGCTGCACCACCCGGCCCAAGGCTCAGCCGCTGGGACTCCCTACCCTTCCT
  CAGCCTCTCTCCGCGGCTGCCGGGAAAGCAAGATGCCGCGCAGGAAGGGCCCCCAACACCCTCCGCCGCC
  CAGTGGCCCCGAGGAGCCTGGGGAGAAGCGCCCCAAGTTTGAAGATATGAATTTAAATGAAGATAAAAAG
  GCACCATTGCGGGAAAAGGACTTCAGTATCAAAAAAGAAATGGTGATGCAGTACATTAATACTGCTTCTA
  AGACAGGAAGTCTTAAGAGAAGCCGACAGATCTCACCTCAGGAATTCATTCATGAGCTGAAAATGGGGTC
  TGCAGATGAGAGACTTGTCACATGCCTGGAGTCTCTCCGAGTGTCTTTGACCAGCAATCCTGTGAGTTGG
  GTGGAAAGCTTTGGACATGAAGGGCTTGGATTATTATTAGACATTTTGGAAAAACTGATTAGTGGAAAAA
  TCCAAGAAAAAGTTGTAAAGAAAAATCAACATAAAGTCATACAGTGTCTAAAAGCCCTGATGAATACGCA
  GTATGGCTTGGAAAGAATTATGAGTGAGGAGAGGAGCCTTTCCTTATTGGCCAAAGCCGTGGATCCCAGA
  CACCCCAATATGATGACAGATGTGGTTAAACTTCTCTCTGCGGTATGCATTGTAGGGGAAGAAAGCATCC
  TTGAAGAAGTTTTAGAAGCTTTAACTTCAGCTGGTGAAGAAAAAAAAATTGACAGATTTTTTTGTATTGT
  GGAAGGCCTCCGGCACAATTCAGTTCAACTGCAAGTAGCTTGTATGCAGCTCATCAATGCCCTGGTTACA
  TCTCCTGATGATTTGGATTTCAGGCTTCACATCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATAT
  TGCCAAATTTAAAATGCATTAAGAATGATGGCCTGGATATCCAACTTAAAGTCTTTGATGAGCATAAAGA
  AGAAGATTTGTTTGAGTTATCCCATCGCCTTGAAGATATTAGAGCTGAACTTGATGAAGCATATGATGTT
  TACAACATGGTGTGGAGCACAGTTAAAGAAACTAGAGCAGAGGGATATTTTATTTCTATTCTTCAGCATC
  TTTTGCTGATTCGAAATGATTATTTTATAAGGCAACAATACTTCAAATTAATTGATGAGTGTGTATCCCA
  GATTGTATTGCATAGAGATGGAATGGATCCAGACTTCACATATCGAAAAAGACTAGATTTAGATTTAACC
  CAGTTTGTAGACATTTGCATAGATCAAGCAAAACTAGAAGAGTTTGAAGAGAAAGCATCAGAACTTTACA
  AGAAATTTGAAAAAGAGTTTACCGACCACCAAGAAACTCAGGCTGAATTGCAGAAAAAAGAGGCAAAGAT
  TAATGAGCTTCAAGCAGAGCTACAAGCTTTTAAGTCTCAGTTTGGTGCCTTGCCAGCTGATTGTAATATT
  CCTTTGCCTCCCTCTAAAGAAGGTGGAACTGGCCACTCAGCACTTCCTCCTCCGCCTCCACTGCCTTCTG
  GTGGAGGGGTGCCGCCTCCACCTCCTCCCCCACCACCTCCTCCACTTCCAGGAATGCGGATGCCATTCAG
  TGGTCCTGTGCCTCCACCACCTCCCCTGGGATTCCTTGGAGGACAAAATTCTCCTCCTCTACCAATCCTG
  CCATTTGGGTTGAAACCAAAGAAAGAATTTAAACCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGA
  TCAGACCTCATGAAATGACTGAAAACTGTTTCTGGATAAAAGTAAATGAAAATAAGTATGAAAACGTGGA
  TTTGCTTTGTAAACTTGAGAATACATTTTGTTGCCAACAAAAAGAGAGAAGAGAAGAGGAAGATATTGAA
  GAGAAGAAATCGATTAAGAAAAAAATTAAAGAACTTAAGTTTTTAGATTCTAAAATTGCCCAGAACCTTT
  CAATCTTCCTGAGCTCTTTTCGGGTGCCATATGAGGAAATCAGAATGATGATATTGGAAGTAGATGAAAC
  ACGGTTGGCAGAGTCTATGATTCAGAACTTAATAAAGCATCTTCCTGATCAAGAGCAATTAAATTCATTG
  TCTCAGTTCAAGAGTGAATATAGCAACTTATGTGAACCTGAGCAGTTTGTGGTTGTGATGAGCAATGTGA
  AGAGACTACGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAGTTTGAAGAGCAGGTGAACAACATCAA
  ACCTGACATCATGGCTGTCAGTACTGCCTGCGAAGAGATAAAGAAGAGCAAAAGCTTTAGCAAGTTGCTG
  GAACTTGTATTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTAACC
  TTAGCTCTCTCTGTAAACTAAAGGACACAAAATCAGCAGATCAGAAAACAACGCTACTTCATTTCCTGGT
  AGAAATATGTGAAGAGAAGTACCCTGATATACTGAATTTTGTGGATGATTTGGAACCTTTAGACAAAGCT
  AGTAAAGTCTCTGTAGAAACGCTGGAAAAGAATTTGAGGCAGATGGGAAGGCAGCTTCAACAGCTTGAGA
  AGGAATTGGAAACCTTTCCCCCTCCTGAGGACTTGCATGACAAGTTTGTGACAAAGATGTCCAGATTTGT
  TATCAGTGCAAAAGAACAATATGAGACACTTTCGAAGTTACACGAAAACATGGAAAAGTTATACCAGAGT
  ATAATAGGATACTATGCCATTGATGTGAAGAAGGTGTCTGTGGAAGACTTTCTTACTGACCTGAATAACT
  TCAGAACCACATTCATGCAAGCAATAAAGGAGAATATCAAAAAAAGAGAAGCAGAGGAAAAAGAAAAACG
  TGTCAGAATAGCTAAAGAATTAGCAGAGCGAGAAAGACTCGAACGCCAACAAAAGAAAAAGCGTTTATTA
  GAAATGAAGACTGAGGGTGATGAGACAGGAGTGATGGATAATCTGCTGGAGGCCTTGCAGTCCGGGGCTG
  CCTTCCGCGACAGAAGAAAAAGGACACCGATGCCAAAAGATGTTCGGCAGAGTCTCAGTCCAATGTCTCA
  GAGGCCTGTTCTGAAAGTTTGTAACCATGAAAATCAGAAAGTGCAGTTGACAGAAGGGTCACGTTCACAC
  TACAATATCAATTGCAACTCAACAAGGACTCCAGTCGCCAAGGAGCTTAATTATAATCTAGACACTCATA
  CGTCTACTGGGAGGATCAAGGCAGCTGAGAAGAAGGAAGCGTGTAATGTAGAAAGCAACAGAAAAAAGGA
  AACGGAACTTCTTGGCTCTTTTTCTAAAAATGAATCAGTTCCCGAAGTTGAAGCCCTGCTGGCAAGATTA
  CGAGCTTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235176 representing NM_001258368
  Red=Cloning site Green=Tags(s)
  
  MERHQPRLHHPAQGSAAGTPYPSSASLRGCRESKMPRRKGPQHPPPPSGPEEPGEKRPKFEDMNLNEDKK
  APLREKDFSIKKEMVMQYINTASKTGSLKRSRQISPQEFIHELKMGSADERLVTCLESLRVSLTSNPVSW
  VESFGHEGLGLLLDILEKLISGKIQEKVVKKNQHKVIQCLKALMNTQYGLERIMSEERSLSLLAKAVDPR
  HPNMMTDVVKLLSAVCIVGEESILEEVLEALTSAGEEKKIDRFFCIVEGLRHNSVQLQVACMQLINALVT
  SPDDLDFRLHIRNEFMRCGLKEILPNLKCIKNDGLDIQLKVFDEHKEEDLFELSHRLEDIRAELDEAYDV
  YNMVWSTVKETRAEGYFISILQHLLLIRNDYFIRQQYFKLIDECVSQIVLHRDGMDPDFTYRKRLDLDLT
  QFVDICIDQAKLEEFEEKASELYKKFEKEFTDHQETQAELQKKEAKINELQAELQAFKSQFGALPADCNI
  PLPPSKEGGTGHSALPPPPPLPSGGGVPPPPPPPPPPPLPGMRMPFSGPVPPPPPLGFLGGQNSPPLPIL
  PFGLKPKKEFKPEISMRRLNWLKIRPHEMTENCFWIKVNENKYENVDLLCKLENTFCCQQKERREEEDIE
  EKKSIKKKIKELKFLDSKIAQNLSIFLSSFRVPYEEIRMMILEVDETRLAESMIQNLIKHLPDQEQLNSL
  SQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKSFSKLL
  ELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEKYPDILNFVDDLEPLDKA
  SKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQS
  IIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKELAERERLERQQKKKRLL
  EMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKVCNHENQKVQLTEGSRSH
  YNINCNSTRTPVAKELNYNLDTHTSTGRIKAAEKKEACNVESNRKKETELLGSFSKNESVPEVEALLARL
  RAL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258368
• ORF Size: 3369 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001258368.1, NP_001245297.1
• RefSeq Size: 4602
• RefSeq ORF: 3372
• Locus ID: 81624
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]