PASK (NM_001252122) Human Tagged ORF Clone

CAT#: RG235255

PASK (GFP-tagged) - Homo sapiens PAS domain containing serine/threonine kinase (PASK), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PASK
• Synonyms: PASKIN; STK37
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235255 representing NM_001252122
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACGGGGGCTTAACAGCCTTTGAAGAGGACCAGAGATGCCTTTCCCAGAGCCTCCCCTTGCCAG
  TGTCAGCAGAGGGCCCAGCTGCACAGACCACTGCTGAGCCCAGCAGGTCGTTTTCCTCAGCCCACAGACA
  CCTGAGCAGAAGGAATGGGCTTTCCAGACTCTGCCAGAGCAGGACAGCGCTCTCTGAAGACAGATGGAGC
  TCCTATTGTCTATCATCACTGGCTGCCCAGAATATTTGTACAAGTAAACTGCACTGCCCTGCTGCCCCTG
  AGCACACGGACCCGTCCGAACCGCGGGGCAGTGTGTCCTGCTGCTCCCTGCTGCGGGGACTGTCCTCAGG
  GTGGTCCTCACCTCTGCTTCCGGCCCCTGTGTGCAACCCTAACAAGGCCATCTTCACGGTGGATGCCAAG
  ACCACAGAGATCCTGGTTGCTAACGACAAAGCTTGCGGGCTCCTGGGGTACAGCAGCCAGGACCTGATTG
  GCCAGAAGCTCACGCAGTTCTTTCTGAGGTCAGATTCTGATGTGGTGGAGGCCCTCAGCGAGGAGCACAT
  GGAGGCCGACGGCCACGCTGCGGTGGTGTTTGGCACGGTGGTGGACATCATCAGCCGTAGTGGGGAGAAG
  ATTCCAGTGTCTGTGTGGATGAAGAGGATGCGGCAGGAGCGCCGCCTATGCTGCGTGGTGGTCCTGGAGC
  CCGTGGAGAGGGTCTCGACCTGGGTCGCTTTCCAGAGCGATCTCCCTCCTTCTGGCCAGCACATCCCAAA
  GAATCTCAAGATTCAGAGGTCTGTTGGAAGAGCCAGGGACGGTACCACCTTCCCTCTGAGCTTAAAGCTG
  AAATCCCAACCCAGCAGCGAGGAGGCGACCACCGGTGAGGCGGCCCCTGTGAGCGGCTACCGGGCATCTG
  TCTGGGTGTTCTGCACCATCAGTGGCCTCATCACCCTCCTGCCGGATGGGACCATCCACGGCATCAACCA
  CAGCTTCGCGCTGACACTGTTTGGTTACGGAAAGACGGAGCTCCTGGGCAAGAATATCACTTTCCTGATT
  CCTGGTTTCTACAGCTACATGGACCTTGCGTACAACAGCTCATTACAGCTCCCAGACCTGGCCAGCTGCC
  TGGACGTCGGCAATGAGAGTGGGTGTGGGGAGAGAACCTTGGACCCGTGGCAGGGCCAGGACCCAGCTGA
  GGGGGGCCAGGATCCAAGGATTAATGTCGTGCTTGCTGGTGGCCACGTTGTGCCCCGAGATGAGATCCGG
  AAGCTGATGGAAAGCCAAGACATCTTCACCGGGACTCAGACTGAGCTGATTGCTGGAGGCCAGCTCCTTT
  CCTGCCTCTCACCTCAGCCTGCTCCAGGGGTGGACAATGTCCCAGAAGGAAGCCTGCCAGTGCACGGTGA
  ACAGGCGCTGCCCAAGGACCAGCAAATCACTGCCTTGGGGAGAGAGGAACCTGTGGCAATAGAGAGCCCC
  GGACAGGATCTTCTGGGAGAAAGCAGGTCTGAACCAGTGGATGTGAAGCCATTTGCTTCCTGCGAAGATT
  CTGAAGCTCCAGTCCCAGCTGAGGATGGGGGCAGTGATGCTGGCATGTGTGGCCTGTGTCAGAAGGCCCA
  GCTAGAGCGGATGGGAGTCAGTGGTCCCAGCGGTTCAGACCTTTGGGCTGGGGCTGCCGTGGCCAAGCCC
  CAGGCCAAGGGTCAGCTGGCGGGGGGCAGCCTCCTGATGCACTGCCCTTGCTATGGGAGTGAATGGGGCT
  TGTGGTGGCGAAGCCAGGACTTGGCCCCCAGCCCCTCTGGGATGGCAGGCCTCTCGTTTGGGACACCTAC
  TCTAGATGAGCCGTGGCTGGGAGTGGAAAACGACCGAGAAGAGCTGCAGACCTGCTTGATTAAGGAGCAG
  CTGTCCCAGTTGAGCCTTGCAGGAGCCCTGGATGTCCCCCACGCCGAACTCGTTCCGACAGAGTGCCAGG
  CTGTCACCGCTCCTGTGTCGTCCTGCGATCTGGGAGGCAGAGACCTGTGCGGTGGCTGCACGGGCAGCTC
  CTCAGCCTGCTATGCCTTGGCCACGGACCTCCCTGGGGGCCTGGAAGCAGTGGAGGCCCAGGAGGTTGAT
  GTGAATTCGTTTTCCTGGAACCTCAAGGAACTCTTTTTCAGTGACCAGACAGACCAAACGTCATCAAATT
  GTTCCTGTGCTACGTCTGAACTCAGAGAGACACCCTCTTCCTTGGCAGTGGGCTCCGATCCAGATGTAGG
  CAGTCTCCAGGAACAGGGGTCGTGTGTCCTGGATGACAGGGAGCTGTTACTACTGACCGGCACCTGTGTT
  GACCTTGGCCAAGGCCGACGGTTCCGGGAGAGCTGTGTGGGACATGATCCAACAGAACCGCTTGAGGTTT
  GTTTGGTGTCCTCTGAGCATTATGCAGCAAGCGACAGAGAAAGCCCAGGACACGTTCCTTCCACGTTGGA
  TGCTGGCCCTGAGGACACGTGCCCATCAGCAGAGGAGCCAAGGCTGAACGTCCAGGTCACCTCCACGCCC
  GTGATCGTGATGCGCGGGGCTGCTGGCCTGCAGCGGGAGATCCAGGAGGGTGCCTACTCCGGGAGCTGCT
  ACCATCGAGATGGCTTACGGCTGAGTATACAGTTTGAGGTGAGGCGGGTGGAGCTCCAGGGCCCCACACC
  TCTGTTCTGCTGCTGGCTGGTGAAAGACCTCCTCCACAGCCAACGCGACTCAGCCGCCAGGACCCGCCTG
  TTCCTTGCCAGCCTGCCCGGCTCCACCCACTCTACCGCTGCTGAGCTCACCGGACCCAGCCTGGTGGAAG
  TGCTCAGAGCCAGACCCTGGTTTGAGGAGCCCCCCAAGGCTGTGGAACTGGAGGGGTTGGCGGCCTGTGA
  GGGCGAGTACTCCCAAAAGTACAGTACCATGAGCCCGCTGGGCAGTGGGGCCTTCGGCTTCGTGTGGACT
  GCTGTGGACAAGGAAAAAAACAAGGAGGTGGTGGTGAAGTTTATTAAGAAGGAGAAGGTCTTGGAGGATT
  GTTGGATTGAGGATCCCAAACTTGGGAAAGTTACTTTAGAGATCGCAATTCTATCCAGGGTGGAGCACGC
  CAATATCATCAAGGTATTGGATATATTTGAAAACCAAGGGTTCTTCCAGCTTGTGATGGAGAAGCACGGC
  TCCGGCCTAGACCTCTTCGCTTTCATCGACCGCCACCCCAGGCTGGATGAGCCCCTGGCGAGCTACATCT
  TCCGACAACTAGTGTCAGCAGTGGGATACCTGCGCTTGAAGGACATCATCCACCGTGACATCAAGGATGA
  GAACATCGTGATCGCCGAGGACTTCACAATCAAGCTGATAGACTTTGGCTCGGCCGCCTACTTGGAAAGG
  GGAAAATTATTTTATACTTTTTGTGGGACCATCGAGTACTGTGCACCGGAAGTTCTCATGGGGAATCCCT
  ACAGAGGGCCGGAGCTGGAGATGTGGTCTCTGGGAGTCACTCTGTACACGCTGGTCTTTGAGGAGAACCC
  CTTCTGTGAGCTGGAGGAGACCGTGGAGGCTGCCATACACCCGCCATACCTGGTGTCCAAAGAACTCATG
  AGCCTTGTGTCTGGGCTGCTGCAGCCAGTCCCTGAGAGACGCACCACCTTGGAGAAGCTGGTGACAGACC
  CGTGGGTAACACAGCCTGTGAATCTTGCTGACTATACATGGGAAGAGGTGTTTCGAGTAAACAAGCCAGA
  AAGTGGAGTTCTGTCCGCTGCGAGCCTGGAGATGGGGAACAGGAGCCTGAGTGATGTGGCCCAGGCTCAG
  GAGCTTTGTGGGGGCCCCGTTCCAGGCGAGGCTCCTAATGGCCAAGGCTGTTTGCATCCCGGGGATCCCC
  GTCTGCTGACCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235255 representing NM_001252122
  Red=Cloning site Green=Tags(s)
  
  MEDGGLTAFEEDQRCLSQSLPLPVSAEGPAAQTTAEPSRSFSSAHRHLSRRNGLSRLCQSRTALSEDRWS
  SYCLSSLAAQNICTSKLHCPAAPEHTDPSEPRGSVSCCSLLRGLSSGWSSPLLPAPVCNPNKAIFTVDAK
  TTEILVANDKACGLLGYSSQDLIGQKLTQFFLRSDSDVVEALSEEHMEADGHAAVVFGTVVDIISRSGEK
  IPVSVWMKRMRQERRLCCVVVLEPVERVSTWVAFQSDLPPSGQHIPKNLKIQRSVGRARDGTTFPLSLKL
  KSQPSSEEATTGEAAPVSGYRASVWVFCTISGLITLLPDGTIHGINHSFALTLFGYGKTELLGKNITFLI
  PGFYSYMDLAYNSSLQLPDLASCLDVGNESGCGERTLDPWQGQDPAEGGQDPRINVVLAGGHVVPRDEIR
  KLMESQDIFTGTQTELIAGGQLLSCLSPQPAPGVDNVPEGSLPVHGEQALPKDQQITALGREEPVAIESP
  GQDLLGESRSEPVDVKPFASCEDSEAPVPAEDGGSDAGMCGLCQKAQLERMGVSGPSGSDLWAGAAVAKP
  QAKGQLAGGSLLMHCPCYGSEWGLWWRSQDLAPSPSGMAGLSFGTPTLDEPWLGVENDREELQTCLIKEQ
  LSQLSLAGALDVPHAELVPTECQAVTAPVSSCDLGGRDLCGGCTGSSSACYALATDLPGGLEAVEAQEVD
  VNSFSWNLKELFFSDQTDQTSSNCSCATSELRETPSSLAVGSDPDVGSLQEQGSCVLDDRELLLLTGTCV
  DLGQGRRFRESCVGHDPTEPLEVCLVSSEHYAASDRESPGHVPSTLDAGPEDTCPSAEEPRLNVQVTSTP
  VIVMRGAAGLQREIQEGAYSGSCYHRDGLRLSIQFEVRRVELQGPTPLFCCWLVKDLLHSQRDSAARTRL
  FLASLPGSTHSTAAELTGPSLVEVLRARPWFEEPPKAVELEGLAACEGEYSQKYSTMSPLGSGAFGFVWT
  AVDKEKNKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVLDIFENQGFFQLVMEKHG
  SGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGYLRLKDIIHRDIKDENIVIAEDFTIKLIDFGSAAYLER
  GKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLGVTLYTLVFEENPFCELEETVEAAIHPPYLVSKELM
  SLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADYTWEEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQ
  ELCGGPVPGEAPNGQGCLHPGDPRLLTS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252122
• ORF Size: 3864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001252122.1, NP_001239051.1
• RefSeq Size: 4483
• RefSeq ORF: 3867
• Locus ID: 23178
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Gene Summary: This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]