CACNA1E (NM_001205294) Human Tagged ORF Clone

CAT#: RG235378

CACNA1E (GFP-tagged) - Homo sapiens calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CACNA1E
• Synonyms: BII; CACH6; CACNL1A6; Cav2.3; EIEE69; gm139
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235378 representing NM_001205294
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGCTTCGGGGAGGCGGTGGTCGCCAGGCCAGGGTCCGGCGATGGAGACTCGGACCAGAGCAGGA
  ACCGGCAAGGAACCCCCGTGCCGGCCTCGGGGCAGGCGGCCGCCTACAAGCAGACGAAAGCACAGAGGGC
  GCGGACTATGGCTTTGTACAACCCCATTCCCGTCCGGCAGAACTGTTTCACCGTCAACAGATCCCTGTTC
  ATCTTCGGAGAAGATAACATTGTCAGGAAATATGCCAAGAAGCTCATCGATTGGCCGCCATTTGAGTACA
  TGATCCTGGCCACCATCATTGCCAACTGCATCGTCCTGGCCCTGGAGCAGCATCTTCCTGAGGATGACAA
  GACCCCCATGTCCCGAAGACTGGAGAAGACAGAACCTTATTTCATTGGGATCTTTTGCTTTGAAGCTGGG
  ATCAAAATTGTGGCCCTGGGGTTCATCTTCCATAAGGGCTCTTACCTCCGCAATGGCTGGAATGTCATGG
  ACTTCATCGTGGTCCTCAGTGGCATCCTGGCCACTGCAGGAACCCACTTCAATACTCACGTGGACCTGAG
  GACCCTCCGGGCTGTGCGTGTCCTGCGGCCTTTGAAGCTCGTGTCAGGGATACCTAGCCTGCAGATTGTG
  TTGAAGTCCATCATGAAGGCCATGGTACCTCTTCTGCAGATTGGCCTTCTGCTCTTCTTTGCCATCCTGA
  TGTTTGCTATCATTGGTTTGGAGTTCTACAGTGGCAAGTTACATCGAGCATGCTTCATGAACAATTCAGG
  TATTCTAGAAGGATTTGACCCCCCTCACCCATGTGGTGTGCAGGGCTGCCCAGCTGGTTATGAATGCAAG
  GACTGGATCGGCCCCAATGATGGGATCACCCAGTTTGATAACATCCTTTTTGCTGTGCTGACTGTCTTCC
  AGTGCATCACCATGGAAGGGTGGACCACTGTGCTGTACAATACCAATGATGCCTTAGGAGCCACCTGGAA
  TTGGCTGTACTTCATCCCCCTCATCATCATTGGATCCTTCTTTGTTCTCAACCTAGTCCTGGGAGTGCTT
  TCCGGGGAATTTGCCAAAGAGAGAGAGAGAGTGGAGAACCGAAGGGCTTTCATGAAGCTGCGGCGCCAGC
  AGCAGATTGAGCGTGAGCTGAATGGCTACCGTGCCTGGATAGACAAAGCAGAGGAAGTCATGCTCGCTGA
  AGAAAATAAAAATGCTGGAACATCCGCCTTAGAAGTGCTTCGAAGGGCAACCATCAAGAGGAGCCGGACA
  GAGGCCATGACTCGAGACTCCAGTGATGAGCACTGTGTTGATATCTCCTCTGTGGGCACACCTCTGGCCC
  GAGCCAGTATCAAAAGTGCAAAGGTAGACGGGGTCTCTTATTTCCGGCACAAGGAAAGGCTTCTGCGCAT
  CTCCATTCGCCACATGGTTAAATCCCAGGTGTTTTACTGGATTGTGCTGAGCCTTGTGGCACTCAACACT
  GCCTGTGTGGCCATTGTCCATCACAACCAGCCCCAGTGGCTCACCCACCTCCTCTACTATGCAGAATTTC
  TGTTTCTGGGACTCTTCCTCTTGGAGATGTCCCTGAAGATGTATGGCATGGGGCCTCGCCTTTATTTTCA
  CTCTTCATTCAACTGCTTTGATTTTGGGGTCACAGTGGGCAGTATCTTTGAAGTGGTCTGGGCAATCTTC
  AGACCTGGTACGTCTTTTGGAATCAGTGTCTTGCGAGCCCTCCGGCTTCTAAGAATATTTAAAATAACCA
  AGTATTGGGCTTCCCTACGGAATTTGGTGGTCTCCTTGATGAGCTCAATGAAGTCTATCATCAGTTTGCT
  TTTCCTCCTCTTCCTCTTCATCGTTGTCTTTGCTCTCCTAGGAATGCAGTTATTTGGAGGCAGGTTTAAC
  TTTAATGATGGGACTCCTTCGGCAAATTTTGATACCTTCCCTGCAGCCATCATGACTGTGTTCCAGATCC
  TGACGGGTGAGGACTGGAATGAGGTGATGTACAATGGGATCCGCTCCCAGGGTGGGGTCAGCTCAGGCAT
  GTGGTCTGCCATCTACTTCATTGTGCTCACCTTGTTTGGCAACTACACGCTACTGAATGTGTTCTTGGCT
  ATCGCTGTGGATAATCTCGCCAACGCCCAGGAACTGACCAAGGATGAACAGGAGGAAGAAGAGGCCTTCA
  ACCAGAAACATGCACTGCAGAAGGCCAAGGAGGTCAGCCCGATGTCTGCACCCAACATGCCTTCGATCGA
  GAGGGAGCGGAGGCGCCGGCACCACATGTCCGTGTGGGAGCAGCGTACCAGCCAGCTGAGGAAGCACATG
  CAGATGTCCAGCCAGGAGGCCCTCAACAGAGAGGAGGCGCCGACCATGAACCCGCTCAACCCCCTCAACC
  CGCTCAGCTCCCTCAACCCGCTCAATGCCCACCCCAGCCTTTATCGGCGACCCAGGGCCATTGAGGGCCT
  GGCCCTGGGCCTGGCCCTGGAGAAGTTCGAGGAGGAGCGCATCAGCCGTGGGGGGTCCCTCAAGGGGGAT
  GGAGGGGACCGATCCAGTGCCCTGGACAACCAGAGGACCCCTTTGTCCCTGGGCCAGCGGGAGCCACCAT
  GGCTGGCCAGGCCCTGTCATGGAAACTGTGACCCGACTCAGCAGGAGGCAGGGGGAGGAGAGGCTGTGGT
  GACCTTTGAGGACCGGGCCAGGCACAGGCAGAGCCAACGGCGCAGCCGGCATCGCCGCGTCAGGACAGAA
  GGCAAGGAGTCCTCTTCAGCCTCCCGGAGCAGGTCTGCCAGCCAGGAACGCAGTCTGGATGAAGCCATGC
  CCACTGAAGGGGAGAAGGACCATGAGCTCAGGGGCAACCATGGTGCCAAGGAGCCAACGATCCAAGAAGA
  GAGAGCCCAGGATTTAAGGAGGACCAACAGTCTGATGGTGTCCAGAGGCTCCGGGCTGGCAGGAGGCCTT
  GATGAGGCTGACACCCCCCTAGTCCTGCCCCATCCTGAGCTGGAAGTGGGGAAGCACGTGGTGCTGACGG
  AGCAGGAGCCAGAAGGCAGCAGTGAGCAGGCCCTGCTGGGGAATGTGCAGCTAGACATGGGCCGGGTCAT
  CAGCCAGAGCGAGCCTGACCTCTCCTGCATCACGGCCAACACGGACAAGGCCACCACCGAGAGCACCAGC
  GTCACCGTCGCCATCCCCGACGTGGACCCCTTGGTGGACTCAACCGTGGTGCACATTAGCAACAAGACGG
  ATGGGGAAGCCAGTCCCTTGAAGGAGGCAGAGATCAGAGAGGATGAGGAGGAGGTGGAGAAGAAGAAGCA
  GAAGAAGGAGAAGCGTGAGACAGGCAAAGCCATGGTGCCCCACAGCTCAATGTTCATCTTCAGCACCACC
  AACCCGATCCGGAGGGCCTGCCACTACATCGTGAACCTGCGCTACTTTGAGATGTGCATCCTCCTGGTGA
  TTGCAGCCAGCAGCATCGCCCTGGCGGCAGAGGACCCCGTCCTGACCAACTCGGAGCGCAACAAAGTCCT
  GAGGTATTTTGACTATGTGTTCACGGGCGTGTTCACCTTTGAGATGGTTATAAAGATGATAGACCAAGGC
  TTGATCCTGCAGGATGGGTCCTACTTCCGAGACTTGTGGAACATCCTGGACTTTGTGGTGGTCGTTGGCG
  CATTGGTGGCCTTTGCTCTGGCGAACGCTTTGGGAACCAACAAAGGACGGGACATCAAGACCATCAAGTC
  TCTGCGGGTGCTCCGAGTTCTAAGGCCACTGAAAACCATCAAGCGCTTGCCCAAGCTCAAGGCCGTCTTC
  GACTGCGTAGTGACCTCCTTGAAGAATGTCTTCAACATACTCATTGTGTACAAGCTCTTCATGTTCATCT
  TTGCTGTCATCGCAGTTCAGCTCTTCAAGGGAAAGTTCTTTTATTGCACGGACAGTTCCAAGGACACAGA
  GAAGGAGTGCATAGGCAACTATGTAGATCATGAGAAAAACAAGATGGAGGTGAAGGGCCGGGAATGGAAG
  CGCCATGAATTCCACTACGACAACATTATCTGGGCCCTGCTGACCCTCTTCACCGTCTCCACAGGGGAAG
  GATGGCCTCAAGTTCTGCAGCACTCTGTAGATGTGACAGAGGAAGACCGAGGCCCAAGCCGCAGCAACCG
  CATGGAGATGTCTATCTTTTATGTAGTCTACTTTGTGGTCTTCCCCTTCTTCTTTGTCAATATCTTTGTG
  GCTCTCATCATCATCACCTTCCAGGAGCAAGGGGATAAGATGATGGAGGAGTGCAGCCTGGAGAAGAATG
  AGAGGGCGTGCATCGACTTCGCCATCAGCGCCAAACCTCTCACCCGCTACATGCCGCAGAACAGACACAC
  CTTCCAGTACCGCGTGTGGCACTTTGTGGTGTCTCCGTCCTTTGAGTACACCATTATGGCCATGATCGCC
  TTGAATACTGTTGTGCTGATGATGAAGTATTATTCTGCTCCCTGTACCTATGAGCTGGCCCTGAAGTACC
  TGAATATCGCCTTCACCATGGTGTTTTCCCTGGAATGTGTCCTGAAGGTCATCGCTTTTGGCTTTTTGAA
  CTATTTCCGAGACACCTGGAATATCTTTGACTTCATCACCGTGATTGGCAGTATCACAGAAATTATCCTG
  ACAGACAGCAAGCTGGTGAACACCAGTGGCTTCAATATGAGCTTTCTGAAGCTCTTCCGAGCTGCCCGCC
  TCATAAAGCTCCTGCGTCAGGGCTATACCATACGCATTTTGCTGTGGACCTTTGTGCAGTCCTTTAAGGC
  CCTCCCTTATGTCTGCCTTTTAATTGCCATGCTTTTCTTCATTTATGCCATCATTGGGATGCAGGTATTT
  GGAAACATAAAATTAGACGAGGAGAGTCACATCAACCGGCACAACAACTTCCGGAGTTTCTTTGGGTCCC
  TAATGCTACTCTTCAGGAGTGCCACAGGTGAGGCCTGGCAGGAGATTATGCTGTCATGCCTTGGGGAGAA
  GGGCTGTGAGCCTGACACCACCGCACCATCAGGGCAGAACGAGAACGAACGCTGCGGCACCGATCTGGCC
  TACGTGTACTTTGTCTCCTTCATCTTCTTCTGCTCCTTCTTGATGCTCAACCTGTTTGTGGCCGTCATCA
  TGGACAACTTTGAGTACCTGACTCGGGACTCCTCCATCCTGGGGCCTCACCACTTGGACGAGTTTGTCCG
  CGTCTGGGCAGAATATGACCGAGCAGCATGTGGCCGCATCCATTACACTGAGATGTATGAAATGCTGACT
  CTCATGTCACCTCCGCTAGGCCTCGGCAAGAGATGTCCCTCCAAAGTGGCATATAAGAGGTTGGTCCTGA
  TGAACATGCCAGTAGCTGAGGACATGACGGTCCACTTCACCTCCACACTTATGGCTCTGATCCGGACAGC
  TCTGGACATTAAAATTGCCAAAGGTGGTGCAGACAGGCAGCAGCTAGACTCAGAGCTACAAAAGGAGACC
  CTAGCCATCTGGCCTCACCTATCCCAGAAGATGCTGGATCTGCTTGTGCCCATGCCCAAAGCCTCTGACC
  TGACTGTGGGCAAAATCTATGCAGCAATGATGATCATGGACTACTATAAGCAGAGTAAGGTGAAGAAGCA
  GAGGCAGCAGCTGGAGGAACAGAAAAATGCCCCCATGTTCCAGCGCATGGAGCCTTCATCTCTGCCTCAG
  GAGATCATTGCTAATGCCAAAGCCCTGCCTTACCTCCAGCAGGACCCCGTTTCAGGCCTGAGTGGCCGGA
  GTGGATACCCTTCGATGAGTCCACTCTCTCCCCAGGATATATTCCAGTTGGCTTGTATGGACCCCGCCGA
  TGACGGACAGTTCCAAGAACGGCAGTCTCTGGTGGTGACAGACCCTAGCTCCATGAGACGTTCATTTTCC
  ACTATTCGGGATAAGCGTTCAAATTCCTCGTGGTTGGAGGAATTCTCCATGGAGCGAAGCAGTGAAAATA
  CCTACAAGTCCCGTCGCCGGAGTTACCACTCCTCCTTGCGGCTGTCAGCCCACCGCCTGAACTCTGATTC
  AGGCCACAAGTCTGACACTCACCGCTCAGGGGGCAGGGAGCGGGGACGATCAAAAGAGCGAAAGCATCTT
  CTCTCTCCTGATGTCTCCCGCTGCAATTCAGAAGAGCGAGGGACCCAGGCTGACTGGGAGTCCCCAGAGC
  GCCGTCAATCCAGGTCACCCAGTGAGGGCAGGTCACAGACGCCCAACAGACAGGGCACAGGTTCCCTAAG
  TGAGAGCTCCATCCCCTCTGTCTCTGACACCAGCACCCCAAGAAGAAGTCGTCGGCAGCTCCCACCCGTC
  CCGCCAAAGCCCCGGCCCCTCCTTTCCTACAGCTCCCTGATTCGACACGCGGGCAGCATCTCTCCACCTG
  CTGATGGAAGCGAGGAGGGCTCCCCGCTGACCTCCCAAGCTCTGGAGAGCAACAATGCTTGCCTGACCGA
  GTCTTCCAACTCTCCGCACCCCCAGCAGAGCCAACATGCCTCCCCACAGCGCTACATCTCCGAGCCCTAC
  TTGGCCCTGCACGAAGACTCCCACGCCTCAGACTGTGGTGAGGAGGAGACGCTCACTTTCGAAGCAGCCG
  TGGCTACTAGCCTGGGCCGTTCCAACACCATCGGCTCAGCCCCACCCCTGCGGCATAGCTGGCAGATGCC
  CAACGGGCACTATCGGCGGCGGAGGCGCGGGGGGCCTGGGCCAGGCATGATGTGTGGGGCTGTCAACAAC
  CTGCTAAGTGACACGGAAGAAGATGACAAATGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235378 representing NM_001205294
  Red=Cloning site Green=Tags(s)
  
  MARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLF
  IFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAG
  IKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIV
  LKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECK
  DWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVL
  SGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRT
  EAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNT
  ACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIF
  RPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFN
  FNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLA
  IAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRKHM
  QMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGD
  GGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTE
  GKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGL
  DEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTS
  VTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTT
  NPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQG
  LILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVF
  DCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWK
  RHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFV
  ALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIA
  LNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIIL
  TDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVF
  GNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLA
  YVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLT
  LMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKET
  LAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQ
  EIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFS
  TIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHL
  LSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPV
  PPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPY
  LALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNN
  LLSDTEEDDKC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001205294
• ORF Size: 6753 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205294.1, NP_001192223.1
• RefSeq Size: 14915 bp
• RefSeq ORF: 6756 bp
• Locus ID: 777
• Cytogenetics: 1q25.3
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Calcium signaling pathway, MAPK signaling pathway, Type II diabetes mellitus
• Gene Summary: 'Voltage-dependent calcium channels are multisubunit complexes consisting of alpha-1, alpha-2, beta, and delta subunits in a 1:1:1:1 ratio. These channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This gene encodes the alpha-1E subunit of the R-type calcium channels, which belong to the 'high-voltage activated' group that maybe involved in the modulation of firing patterns of neurons important for information processing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]'