CACNA1G (NM_001256328) Human Tagged ORF Clone

CAT#: RG235380

CACNA1G (GFP-tagged) - Homo sapiens calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant 20

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CACNA1G
• Synonyms: Ca(V)T.1; Cav3.1; NBR13; SCA42; SCA42ND
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235380 representing NM_001256328
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGGAGGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGGAGCTTCATGCGGCTCAACG
  ACCTGTCGGGGGCCGGGGGCCGGCCGGGGCCGGGGTCAGCAGAAAAGGACCCGGGCAGCGCGGACTCCGA
  GGCGGAGGGGCTGCCGTACCCGGCGCTGGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG
  CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCCTGGTTTGAGCGCATCAGCATGTTGGTCATCCTTCTCA
  ACTGCGTGACCCTGGGCATGTTCCGGCCATGCGAGGACATCGCCTGTGACTCCCAGCGCTGCCGGATCCT
  GCAGGCCTTTGATGACTTCATCTTTGCCTTCTTTGCCGTGGAGATGGTGGTGAAGATGGTGGCCTTGGGC
  ATCTTTGGGAAAAAGTGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTCATCGTCATCGCAGGGA
  TGCTGGAGTACTCGCTGGACCTGCAGAACGTCAGCTTCTCAGCTGTCAGGACAGTCCGTGTGCTGCGACC
  GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATCCTTGTCACGTTGCTGCTGGATACGCTGCCCATG
  CTGGGCAACGTCCTGCTGCTCTGCTTCTTCGTCTTCTTCATCTTCGGCATCGTCGGCGTCCAGCTGTGGG
  CAGGGCTGCTTCGGAACCGATGCTTCCTACCTGAGAATTTCAGCCTCCCCCTGAGCGTGGACCTGGAGCG
  CTATTACCAGACAGAGAACGAGGATGAGAGCCCCTTCATCTGCTCCCAGCCACGCGAGAACGGCATGCGG
  TCCTGCAGAAGCGTGCCCACGCTGCGCGGGGACGGGGGCGGTGGCCCACCTTGCGGTCTGGACTATGAGG
  CCTACAACAGCTCCAGCAACACCACCTGTGTCAACTGGAACCAGTACTACACCAACTGCTCAGCGGGGGA
  GCACAACCCCTTCAAGGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC
  ATCACGCTGGAGGGCTGGGTCGACATCATGTACTTTGTGATGGATGCTCATTCCTTCTACAATTTCATCT
  ACTTCATCCTCCTCATCATCGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATTGCCACGCA
  GTTCTCAGAGACCAAGCAGCGGGAAAGCCAGCTGATGCGGGAGCAGCGTGTGCGGTTCCTGTCCAACGCC
  AGCACCCTGGCTAGCTTCTCTGAGCCCGGCAGCTGCTATGAGGAGCTGCTCAAGTACCTGGTGTACATCC
  TTCGTAAGGCAGCCCGCAGGCTGGCTCAGGTCTCTCGGGCAGCAGGTGTGCGGGTTGGGCTGCTCAGCAG
  CCCAGCACCCCTCGGGGGCCAGGAGACCCAGCCCAGCAGCAGCTGCTCTCGCTCCCACCGCCGCCTATCC
  GTCCACCACCTGGTGCACCACCACCACCACCATCACCACCACTACCACCTGGGCAATGGGACGCTCAGGG
  CCCCCCGGGCCAGCCCGGAGATCCAGGACAGGGATGCCAATGGGTCCCGCCGGCTCATGCTGCCACCACC
  CTCGACGCCTGCCCTCTCCGGGGCCCCCCCTGGTGGCGCAGAGTCTGTGCACAGCTTCTACCATGCCGAC
  TGCCACTTAGAGCCAGTCCGCTGCCAGGCGCCCCCTCCCAGGTCCCCATCTGAGGCATCCGGCAGGACTG
  TGGGCAGCGGGAAGGTGTATCCCACCGTGCACACCAGCCCTCCACCGGAGACGCTGAAGGAGAAGGCACT
  AGTAGAGGTGGCTGCCAGCTCTGGGCCCCCAACCCTCACCAGCCTCAACATCCCACCCGGGCCCTACAGC
  TCCATGCACAAGCTGCTGGAGACACAGAGTACAGGTGCCTGCCAAAGCTCTTGCAAGATCTCCAGCCCTT
  GCTTGAAAGCAGACAGTGGAGCCTGTGGTCCAGACAGCTGCCCCTACTGTGCCCGGGCCGGGGCAGGGGA
  GGTGGAGCTCGCCGACCGTGAAATGCCTGACTCAGACAGCGAGGCAGTTTATGAGTTCACACAGGATGCC
  CAGCACAGCGACCTCCGGGACCCCCACAGCCGGCGGCAACGGAGCCTGGGCCCAGATGCAGAGCCCAGCT
  CTGTGCTGGCCTTCTGGAGGCTAATCTGTGACACCTTCCGAAAGATTGTGGACAGCAAGTACTTTGGCCG
  GGGAATCATGATCGCCATCCTGGTCAACACACTCAGCATGGGCATCGAATACCACGAGCAGCCCGAGGAG
  CTTACCAACGCCCTAGAAATCAGCAACATCGTCTTCACCAGCCTCTTTGCCCTGGAGATGCTGCTGAAGC
  TGCTTGTGTATGGTCCCTTTGGCTACATCAAGAATCCCTACAACATCTTCGATGGTGTCATTGTGGTCAT
  CAGCGTGTGGGAGATCGTGGGCCAGCAGGGGGGCGGCCTGTCGGTGCTGCGGACCTTCCGCCTGATGCGT
  GTGCTGAAGCTGGTGCGCTTCCTGCCGGCGCTGCAGCGGCAGCTGGTGGTGCTCATGAAGACCATGGACA
  ACGTGGCCACCTTCTGCATGCTGCTTATGCTCTTCATCTTCATCTTCAGCATCCTGGGCATGCATCTCTT
  CGGCTGCAAGTTTGCCTCTGAGCGGGATGGGGACACCCTGCCAGACCGGAAGAATTTTGACTCCTTGCTC
  TGGGCCATCGTCACTGTCTTTCAGATCCTGACCCAGGAGGACTGGAACAAAGTCCTCTACAATGGTATGG
  CCTCCACGTCGTCCTGGGCGGCCCTTTATTTCATTGCCCTCATGACCTTCGGCAACTACGTGCTCTTCAA
  TTTGCTGGTCGCCATTCTGGTGGAGGGCTTCCAGGCGGAGGAAATCAGCAAACGGGAAGATGCGAGTGGA
  CAGTTAAGCTGTATTCAGCTGCCTGTCGACTCCCAGGGGGGAGATGCCAACAAGTCCGAATCAGAGCCCG
  ATTTCTTCTCACCCAGCCTGGATGGTGATGGGGACAGGAAGAAGTGCTTGGCCTTGGTGTCCCTGGGAGA
  GCACCCGGAGCTGCGGAAGAGCCTGCTGCCGCCTCTCATCATCCACACGGCCGCCACACCCATGTCGCTG
  CCCAAGAGCACCAGCACGGGCCTGGGCGAGGCGCTGGGCCCTGCGTCGCGCCGCACCAGCAGCAGCGGGT
  CGGCAGAGCCTGGGGCGGCCCACGAGATGAAGTCACCGCCCAGCGCCCGCAGCTCTCCGCACAGCCCCTG
  GAGCGCTGCAAGCAGCTGGACCAGCAGGCGCTCCAGCCGGAACAGCCTCGGCCGTGCACCCAGCCTGAAG
  CGGAGAAGCCCAAGTGGAGAGCGGCGGTCCCTGTTGTCGGGAGAAGGCCAGGAGAGCCAGGATGAAGAGG
  AGAGCTCAGAAGAGGAGCGGGCCAGCCCTGCGGGCAGTGACCATCGCCACAGGGGGTCCCTGGAGCGGGA
  GGCCAAGAGTTCCTTTGACCTGCCAGACACACTGCAGGTGCCAGGGCTGCATCGCACTGCCAGTGGCCGA
  GGGTCTGCTTCTGAGCACCAGGACTGCAATGGCAAGTCGGCTTCAGGGCGCCTGGCCCGGGCCCTGCGGC
  CTGATGACCCCCCACTGGATGGGGATGACGCCGATGACGAGGGCAACCTGAGCAAAGGGGAACGGGTCCG
  CGCGTGGATCCGAGCCCGACTCCCTGCCTGCTGCCTCGAGCGAGACTCCTGGTCAGCCTACATCTTCCCT
  CCTCAGTCCAGGTTCCGCCTCCTGTGTCACCGGATCATCACCCACAAGATGTTCGACCACGTGGTCCTTG
  TCATCATCTTCCTTAACTGCATCACCATCGCCATGGAGCGCCCCAAAATTGACCCCCACAGCGCTGAACG
  CATCTTCCTGACCCTCTCCAATTACATCTTCACCGCAGTCTTTCTGGCTGAAATGACAGTGAAGGTGGTG
  GCACTGGGCTGGTGCTTCGGGGAGCAGGCGTACCTGCGGAGCAGTTGGAACGTGCTGGACGGGCTGTTGG
  TGCTCATCTCCGTCATCGACATTCTGGTGTCCATGGTCTCTGACAGCGGCACCAAGATCCTGGGCATGCT
  GAGGGTGCTGCGGCTGCTGCGGACCCTGCGCCCGCTCAGGGTGATCAGCCGGGCGCAGGGGCTGAAGCTG
  GTGGTGGAGACGCTGATGTCCTCACTGAAACCCATCGGCAACATTGTAGTCATCTGCTGTGCCTTCTTCA
  TCATTTTCGGCATCTTGGGGGTGCAGCTCTTCAAAGGGAAGTTTTTCGTGTGCCAGGGCGAGGATACCAG
  GAACATCACCAATAAATCGGACTGTGCCGAGGCCAGTTACCGGTGGGTCCGGCACAAGTACAACTTTGAC
  AACCTTGGCCAGGCCCTGATGTCCCTGTTCGTTTTGGCCTCCAAGGATGGTTGGGTGGACATCATGTACG
  ATGGGCTGGATGCTGTGGGCGTGGACCAGCAGCCCATCATGAACCACAACCCCTGGATGCTGCTGTACTT
  CATCTCGTTCCTGCTCATTGTGGCCTTCTTTGTCCTGAACATGTTTGTGGGTGTGGTGGTGGAGAACTTC
  CACAAGTGTCGGCAGCACCAGGAGGAAGAGGAGGCCCGGCGGCGGGAGGAGAAGCGCCTACGAAGACTGG
  AGAAAAAGAGAAGGAAAGCCCAGTGCAAACCTTACTACTCCGACTACTCCCGCTTCCGGCTCCTCGTCCA
  CCACTTGTGCACCAGCCACTACCTGGACCTCTTCATCACAGGTGTCATCGGGCTGAACGTGGTCACCATG
  GCCATGGAGCACTACCAGCAGCCCCAGATTCTGGATGAGGCTCTGAAGATCTGCAACTACATCTTCACTG
  TCATCTTTGTCTTGGAGTCAGTTTTCAAACTTGTGGCCTTTGGTTTCCGTCGGTTCTTCCAGGACAGGTG
  GAACCAGCTGGACCTGGCCATTGTGCTGCTGTCCATCATGGGCATCACGCTGGAGGAAATCGAGGTCAAC
  GCCTCGCTGCCCATCAACCCCACCATCATCCGCATCATGAGGGTGCTGCGCATTGCCCGAGTGCTGAAGC
  TGCTGAAGATGGCTGTGGGCATGCGGGCGCTGCTGGACACGGTGGGGAACCTGGGACTTCTCTTCATGTT
  GTTGTTTTTCATCTTTGCAGCTCTGGGCGTGGAGCTCTTTGGAGACCTGGAGTGTGACGAGACACACCCC
  TGTGAGGGCCTGGGCCGTCATGCCACCTTTCGGAACTTTGGCATGGCCTTCCTAACCCTCTTCCGAGTCT
  CCACAGGTGACAATTGGAATGGCATTATGAAGGACACCCTCCGGGACTGTGACCAGGAGTCCACCTGCTA
  CAACACGGTCATCTCGCCTATCTACTTTGTGTCCTTCGTGCTGACGGCCCAGTTCGTGCTAGTCAACGTG
  GTGATCGCCGTGCTGATGAAGCACCTGGAGGAGAGCAACAAGGAGGCCAAGGAGGAGGCCGAGCTAGAGG
  CTGAGCTGGAGCTGGAGATGAAGACCCTCAGCCCCCAGCCCCACTCGCCACTGGGCAGCCCCTTCCTCTG
  GCCTGGGGTCGAGGGCCCCGACAGCCCCGACAGCCCCAAGCCTGGGGCTCTGCACCCAGCGGCCCACGCG
  AGATCAGCCTCCCACTTTTCCCTGGAGCACCCCACGATGCAGCCCCACCCCACGGAGCTGCCAGGACCAG
  ACTTACTGACTGTGCGGAAGTCTGGGGTCAGCCGAACGCACTCTCTGCCCAATGACAGCTACATGTGTCG
  GCATGGGAGCACTGCCGAGGGGCCCCTGGGACACAGGGGCTGGGGGCTCCCCAAAGCTCAGTCAGGCTCC
  GTCTTGTCCGTTCACTCCCAGCCAGCAGATACCAGCTACATCCTGCAGCTTCCCAAAGATGCACCTCATC
  TGCTCCAGCCCCACAGCGCCCCAACCTGGGGCACCATCCCCAAACTGCCCCCACCAGGACGCTCCCCTTT
  GGCTCAGAGGCCACTCAGGCGCCAGGCAGCAATAAGGACTGACTCCTTGGACGTTCAGGGTCTGGGCAGC
  CGGGAAGACCTGCTGGCAGAGGTGAGTGGGCCCTCCCCGCCCCTGGCCCGGGCCTACTCTTTCTGGGGCC
  AGTCAAGTACCCAGGCACAGCAGCACTCCCGCAGCCACAGCAAGATCTCCAAGCACATGACCCCGCCAGC
  CCCTTGCCCAGGCCCAGAACCCAACTGGGGCAAGGGCCCTCCAGAGACCAGAAGCAGCTTAGAGTTGGAC
  ACGGAGCTGAGCTGGATTTCAGGAGACCTCCTGCCCCCTGGCGGCCAGGAGGAGCCCCCATCCCCACGGG
  ACCTGAAGAAGTGCTACAGCGTGGAGGCCCAGAGCTGCCAGCGCCGGCCTACGTCCTGGCTGGATGAGCA
  GAGGAGACACTCTATCGCCGTCAGCTGCCTGGACAGCGGCTCCCAACCCCACCTGGGCACAGACCCCTCT
  AACCTTGGGGGCCAGCCTCTTGGGGGGCCTGGGAGCCGGCCCAAGAAAAAACTCAGCCCGCCTAGTATCA
  CCATAGACCCCCCCGAGAGCCAAGGTCCTCGGACCCCGCCCAGCCCTGGTATCTGCCTCCGGAGGAGGGC
  TCCGTCCAGCGACTCCAAGGATCCCTTGGCCTCTGGCCCCCCTGACAGCATGGCTGCCTCGCCCTCCCCA
  AAGAAAGATGTGCTGAGTCTCTCCGGTTTATCCTCTGACCCAGCAGACCTGGACCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235380 representing NM_001256328
  Red=Cloning site Green=Tags(s)
  
  MDEEEDGAGAEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRP
  RSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALG
  IFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPM
  LGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDESPFICSQPRENGMR
  SCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQV
  ITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNA
  STLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPLGGQETQPSSSCSRSHRRLS
  VHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDRDANGSRRLMLPPPSTPALSGAPPGGAESVHSFYHAD
  CHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPETLKEKALVEVAASSGPPTLTSLNIPPGPYS
  SMHKLLETQSTGACQSSCKISSPCLKADSGACGPDSCPYCARAGAGEVELADREMPDSDSEAVYEFTQDA
  QHSDLRDPHSRRQRSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEE
  LTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMR
  VLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLL
  WAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEEISKREDASG
  QLSCIQLPVDSQGGDANKSESEPDFFSPSLDGDGDRKKCLALVSLGEHPELRKSLLPPLIIHTAATPMSL
  PKSTSTGLGEALGPASRRTSSSGSAEPGAAHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLK
  RRSPSGERRSLLSGEGQESQDEEESSEEERASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGR
  GSASEHQDCNGKSASGRLARALRPDDPPLDGDDADDEGNLSKGERVRAWIRARLPACCLERDSWSAYIFP
  PQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVV
  ALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKL
  VVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFD
  NLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENF
  HKCRQHQEEEEARRREEKRLRRLEKKRRKAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTM
  AMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVN
  ASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVGNLGLLFMLLFFIFAALGVELFGDLECDETHP
  CEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNV
  VIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGPDSPDSPKPGALHPAAHA
  RSASHFSLEHPTMQPHPTELPGPDLLTVRKSGVSRTHSLPNDSYMCRHGSTAEGPLGHRGWGLPKAQSGS
  VLSVHSQPADTSYILQLPKDAPHLLQPHSAPTWGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGS
  REDLLAEVSGPSPPLARAYSFWGQSSTQAQQHSRSHSKISKHMTPPAPCPGPEPNWGKGPPETRSSLELD
  TELSWISGDLLPPGGQEEPPSPRDLKKCYSVEAQSCQRRPTSWLDEQRRHSIAVSCLDSGSQPHLGTDPS
  NLGGQPLGGPGSRPKKKLSPPSITIDPPESQGPRTPPSPGICLRRRAPSSDSKDPLASGPPDSMAASPSP
  KKDVLSLSGLSSDPADLDP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256328
• ORF Size: 6777 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001256328.1, NP_001243257.1
• RefSeq Size: 7910
• RefSeq ORF: 6780
• Locus ID: 8913
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Calcium signaling pathway, MAPK signaling pathway, Type II diabetes mellitus
• Gene Summary: Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division, and cell death. This gene encodes a T-type, low-voltage activated calcium channel. The T-type channels generate currents that are both transient, owing to fast inactivation, and tiny, owing to small conductance. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing. Many alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Sep 2011]