LIAS (NM_001278592) Human Tagged ORF Clone
CAT#: RG235592
- TrueORF®
LIAS (GFP-tagged) - Human lipoic acid synthetase (LIAS), transcript variant 5
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | LIAS |
Synonyms | HGCLAS; HUSSY-01; LAS; LIP1; LS; PDHLD |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG235592 representing NM_001278592.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTCTACGCTGCGGGGATGCAGCCCGCACCCTGGGGCCCCGGGTATTTGGGAGATATTTTTGCAGC CCAGTCAGACCGTTAAGCTCCTTGCCAGATAAAAAAAAGGAACTCCTACAGAATGGACCAGACCTTCAA GATTTTGTATCTGGTGATCTTGCAGACAGGAGCACCTGGGATGAATATAAAGGAAACCTAAAACGCCAG AAAGGAGAAAGGTATGTGAGGAAGCTCGATGTCCCAATATTGGAGAGTGTTGGGGAGGTGGAGAATATG CCACCGCCACAGCCACGATCATGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG235592
Blue=ORF Red=Cloning site Green=Tag(s) MSLRCGDAARTLGPRVFGRYFCSPVRPLSSLPDKKKELLQNGPDLQDFVSGDLADRSTWDEYKGNLKRQ KGERYVRKLDVPILESVGEVENMPPPQPRSW TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001278592 |
ORF Size | 300 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001278592.1, NP_001265521.1 |
RefSeq Size | 610 bp |
RefSeq ORF | 303 bp |
Locus ID | 11019 |
Cytogenetics | 4p14 |
Protein Pathways | Lipoic acid metabolism, Metabolic pathways |
MW | 11.8 kDa |
Gene Summary | The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. It localizes in mitochondrion and plays an important role in alpha-(+)-lipoic acid synthesis. It may also function in the sulfur insertion chemistry in lipoate biosynthesis. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008] |
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