ITGB1BP2 (NM_001303277) Human Tagged ORF Clone
CAT#: RG236702
- TrueORF®
ITGB1BP2 (GFP-tagged) - Human integrin beta 1 binding protein (melusin) 2 (ITGB1BP2), transcript variant 2
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | ITGB1BP2 |
Synonyms | CHORDC3; ITGB1BP; MELUSIN; MSTP015 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG236702 representing NM_001303277.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCATTGGAACAGAAGGAATTAGACCAGGAACCTGGGGCAGGACTTGACAGTCTGATCCGGACTGGT TCCAGCTGCCAGAACCCAGGATGTGATGCTGTTTACCAAGGCCCTGAGAGTGATGCTACTCCATGTACC TACCACCCAGGAGCACCCCGATTCCATGAGGGGATGAAGTCTTGGAGCTGTTGTGGCATCCAGACCCTG GATTTTGGGGCATTCTTGGCACAACCAGGGTGCAGAGTCGGTAGACATGACTGGGGGAAGCAGCTCCCA GCATCTTGCCGCCATGATTGGCACCAGACAGATTCCTTAGTAGTGGTGACTGTATATGGCCAGATTCCA CTTCCTGCGTTTAACTGGGTGAAGGCCAGTCAAACTGAGCTTCATGTCCACATTGTCTTTGATGGTAAC CGTGTGTTCCAAGCACAGATGAAGCTCTGGGGGGTCATAAACGTGGAGCAGAGCTCTGTCTTCTTGATG CCATCTCGGGTTGAAATCTCCCTGGTCAAGGCTGACCCAGGATCCTGGGCCCAGCTGGAGCACCCTGAT GCACTAGCTAAGAAGGCTAGGGCAGGGGTTGTGTTAGAGATGGATGAGGAAGAATCTGACGATTCAGAT GATGATCTGAGCTGGACAGAGGAGGAGGAAGAGGAGGAAGCAATGGGGGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG236702
Blue=ORF Red=Cloning site Green=Tag(s) MALEQKELDQEPGAGLDSLIRTGSSCQNPGCDAVYQGPESDATPCTYHPGAPRFHEGMKSWSCCGIQTL DFGAFLAQPGCRVGRHDWGKQLPASCRHDWHQTDSLVVVTVYGQIPLPAFNWVKASQTELHVHIVFDGN RVFQAQMKLWGVINVEQSSVFLMPSRVEISLVKADPGSWAQLEHPDALAKKARAGVVLEMDEEESDDSD DDLSWTEEEEEEEAMGE TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001303277 |
ORF Size | 672 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001303277.3 |
RefSeq Size | 1039 bp |
RefSeq ORF | 675 bp |
Locus ID | 26548 |
Cytogenetics | Xq13.1 |
MW | 25.2 kDa |
Gene Summary | This gene encodes a protein with two cysteine and histidine-rich (CHORD) domains, PXXP motifs, YXXI/P motifs, putative SH2 and SH3 domain binding motifs, and an acidic region at the C-terminus that can bind calcium. Two hybrid analysis showed that this protein interacts with the cytoplasmic domain of the beta 1 integrin subunit and is thought to act as a chaperone protein. Studies in the mouse ortholog of this gene indicate that absence of this gene in mouse results in failed cardiac hypertrophy in response to mechanical stress. Alternative splicing results in multiple transcript variants encoding different isoforms, including an isoform that lacks several domains, including one of the CHORD domains. [provided by RefSeq, May 2017] |
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