Acidic Calponin (CNN3) (NM_001286056) Human Tagged ORF Clone

CAT#: RG237216

CNN3 (GFP-tagged) - Human calponin 3, acidic (CNN3), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CNN3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237216 representing NM_001286056.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGCATTGGCCCCAACTTCCAGCTGGGCTTAAAGGATGGCATCATCCTCTGCGAACTTATAAACAAG
  CTACAGCCAGGCTCAGTGAAGAAGGTCAACGAGTCCTCACTGAACTGGCCTCAGTTGGAGAATATTGGC
  AACTTTATTAAAGCTATTCAGGCTTATGGTATGAAGCCACATGACATATTCGAAGCAAATGATCTTTTT
  GAGAATGGAAACATGACCCAGGTTCAGACTACTCTGGTGGCTCTAGCAGGTCTGGCTAAAACAAAAGGA
  TTCCATACAACCATTGACATTGGAGTTAAGTATGCAGAAAAACAAACAAGACGTTTTGATGAAGGAAAA
  TTAAAAGCTGGCCAAAGTGTAATTGGTCTGCAGATGGGAACCAACAAATGTGCCAGCCAGGCAGGTATG
  ACAGCTTACGGGACTAGGAGGCATCTTTATGATCCCAAAATGCAAACTGACAAACCTTTTGACCAGACC
  ACAATTAGTCTGCAGATGGGCACTAATAAAGGAGCCAGCCAGGCAGGGATGTTAGCACCAGGTACCAGA
  AGAGACATCTATGATCAGAAGCTAACATTACAGCCGGTGGACAACTCGACAATTTCCCTACAGATGGGT
  ACCAACAAAGTTGCTTCCCAGAAAGGAATGAGTGTGTATGGGCTTGGGCGGCAAGTATATGATCCCAAA
  TACTGTGCTGCTCCTACAGAACCTGTCATTCACAACGGAAGCCAAGGAACAGGAACAAATGGTTCGGAA
  ATCAGTGATAGTGATTATCAGGCAGAATACCCTGATGAGTATCATGGCGAGTACCAGGATGACTACCCC
  AGAGATTACCAATATAGCGACCAAGGCATTGATTAT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237216
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDLF
  ENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMGTNKCASQAGM
  TAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIYDQKLTLQPVDNSTISLQMG
  TNKVASQKGMSVYGLGRQVYDPKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYQDDYP
  RDYQYSDQGIDY
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286056
• ORF Size: 864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286056.1, NP_001272985.1
• RefSeq Size: 1902 bp
• RefSeq ORF: 867 bp
• Locus ID: 1266
• Cytogenetics: 1p21.3
• MW: 32.2 kDa
• Gene Summary: 'This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq, Jul 2008]'