Serum Response Factor (SRF) (NM_001292001) Human Tagged ORF Clone

CAT#: RG237323

SRF (GFP-tagged) - Human serum response factor (c-fos serum response element-binding transcription factor) (SRF), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: SRF
• Synonyms: MCM1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237323 representing NM_001292001.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATCACCAGTGAGACCGGCAAGGCACTGATTCAGACCTGCCTCAACTCGCCAGACTCTCCACCCCGT
  TCAGACCCCACAACAGACCAGAGAATGAGTGCCACTGGCTTTGAAGAGACAGATCTCACCTACCAGGTG
  TCGGAGTCTGACAGCAGTGGGGAGACCAAGGACACACTGAAGCCGGCGTTCACAGTCACCAACCTGCCG
  GGTACAACCTCCACCATCCAAACAGCACCTAGCACCTCTACCACCATGCAAGTCAGCAGCGGCCCCTCC
  TTTCCCATCACCAACTACCTGGCACCAGTGTCTGCTAGTGTCAGCCCCAGTGCTGTCAGCAGTGCCAAT
  GGGACTGTGCTGAAGAGTACAGGCAGCGGCCCTGTCTCCTCTGGGGGCCTTATGCAGCTGCCTACCAGC
  TTCACCCTCATGCCTGGTGGGGCAGTGGCCCAGCAGGTCCCAGTGCAGGCCATTCAAGTGCACCAGGCC
  CCACAGCAAGCGTCTCCCTCCCGTGACAGCAGCACAGACCTCACGCAGACCTCCTCCAGCGGGACAGTG
  ACGCTGCCCGCCACCATCATGACGTCATCCGTGCCCACAACTGTGGGTGGCCACATGATGTACCCTAGC
  CCGCATGCGGTGATGTATGCCCCCACCTCGGGCCTGGGTGATGGCAGCCTCACCGTGCTGAATGCCTTC
  TCCCAGGCACCATCCACCATGCAGGTGTCACACAGCCAGGTCCAGGAGCCAGGTGGCGTCCCCCAGGTG
  TTCCTGACAGCATCATCTGGGACAGTGCAGATCCCTGTTTCAGCAGTTCAGCTCCACCAGATGGCTGTG
  ATAGGGCAGCAGGCCGGGAGCAGCAGCAACCTCACCGAGCTACAGGTGGTGAACCTGGACACCGCCCAC
  AGCACCAAGAGTGAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237323
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEETDLTYQVSESDSSGETKDTLKPAFTVTNLP
  GTTSTIQTAPSTSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTS
  FTLMPGGAVAQQVPVQAIQVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPS
  PHAVMYAPTSGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAV
  IGQQAGSSSNLTELQVVNLDTAHSTKSE
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001292001
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001292001.2
• RefSeq Size: 3417 bp
• RefSeq ORF: 915 bp
• Locus ID: 6722
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway
• MW: 31.5 kDa
• Gene Summary: 'This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]'