PTDSS1 (NM_001290225) Human Tagged ORF Clone

CAT#: RG237460

PTDSS1 (GFP-tagged) - Human phosphatidylserine synthase 1 (PTDSS1), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PTDSS1
• Synonyms: LMHD; PSS1; PSSA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237460 representing NM_001290225.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGTATGCTGTGAACTGCCATGTGATCACCTGGGAGAGGATTATCAGCCACTTTGATATTTTTGCA
  TTTGGACATTTCTGGGGCTGGGCCATGAAGGCCTTGCTGATCCGTAGTTACGGTCTCTGCTGGACAATC
  AGTATTACCTGGGAGCTGACTGAGCTCTTCTTCATGCATCTCCTCCCCAATTTTGCCGAGTGCTGGTGG
  GATCAAGTCATTCTGGACATCCTGTTGTGCAATGGCGGTGGCATTTGGCTGGGCATGGTCGTTTGCCGG
  TTTTTAGAGATGAGGACTTACCACTGGGCAAGCTTCAAGGACATTCATACCACCACCGGGAAGATCAAG
  AGAGCTGTTCTGCAGTTCACTCCTGCTAGCTGGACCTATGTTCGATGGTTTGACCCCAAATCTTCTTTT
  CAGAGAGTAGCTGGAGTGTACCTTTTCATGATCATCTGGCAGCTGACTGAGTTGAATACCTTCTTCTTG
  AAGCATATCTTTGTGTTCCAAGCCAGTCATCCATTAAGTTGGGGTAGAATTCTCTTTATTGGTGGCATC
  ACAGCTCCCACAGTGAGACAGTACTACGCTTACCTCACCGACACACAGTGCAAGCGCGTAGGAACACAA
  TGCTGGGTGTTTGGGGTCATTGGTTTCCTGGAGGCCATTGTTTGCATAAAATTTGGACAAGATCTCTTC
  TCTAAGACCCAAATACTCTATGTTGTGCTTTGGCTTCTTTGCGTGGCTTTCACCACTTTCCTCTGTCTG
  TACGGCATGATTTGGTATGCAGAACACTATGGTCACCGAGAAAAGACCTACTCGGAGTGTGAAGATGGC
  ACCTACAGTCCAGAGATCTCCTGGCATCACAGGAAAGGGACAAAAGGTTCTGAAGACAGCCCACCCAAG
  CATGCAGGCAACAACGAAAGCCATTCTTCCAGGAGAAGGAATCGGCATTCCAAGTCAAAAGTCACCAAT
  GGCGTTGGAAAGAAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237460
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEYAVNCHVITWERIISHFDIFAFGHFWGWAMKALLIRSYGLCWTISITWELTELFFMHLLPNFAECWW
  DQVILDILLCNGGGIWLGMVVCRFLEMRTYHWASFKDIHTTTGKIKRAVLQFTPASWTYVRWFDPKSSF
  QRVAGVYLFMIIWQLTELNTFFLKHIFVFQASHPLSWGRILFIGGITAPTVRQYYAYLTDTQCKRVGTQ
  CWVFGVIGFLEAIVCIKFGQDLFSKTQILYVVLWLLCVAFTTFLCLYGMIWYAEHYGHREKTYSECEDG
  TYSPEISWHHRKGTKGSEDSPPKHAGNNESHSSRRRNRHSKSKVTNGVGKK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290225
• ORF Size: 981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001290225.2
• RefSeq Size: 2406 bp
• RefSeq ORF: 984 bp
• Locus ID: 9791
• Cytogenetics: 8q22.1
• Protein Families: Transmembrane
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• MW: 38.7 kDa
• Gene Summary: The protein encoded by this gene catalyzes the formation of phosphatidylserine from either phosphatidylcholine or phosphatidylethanolamine. Phosphatidylserine localizes to the mitochondria-associated membrane of the endoplasmic reticulum, where it serves a structural role as well as a signaling role. Defects in this gene are a cause of Lenz-Majewski hyperostotic dwarfism. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]