GLS2 (NM_001280798) Human Tagged ORF Clone

CAT#: RG237542

GLS2 (GFP-tagged) - Human glutaminase 2 (liver, mitochondrial) (GLS2), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: GLS2
• Synonyms: GA; GLS; hLGA; LGA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237542 representing NM_001280798.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTCAATGCTGGTGCCATTGTTGTCAGCTCCCTGATCAAGATGGACTGTAACAAAGCAGAGAAGTTT
  GATTTTGTGTTGCAGTATCTCAACAAAATGGCTGGGAATGAATACATGGGTTTCAGCAATGCCACATTC
  CAGTCAGAGAAGGAAACAGGGGATCGGAATTATGCCATCGGCTATTATCTCAAGGAAAAGAAGTGCTTT
  CCTAAGGGGGTGGACATGATGGCTGCCCTTGATCTCTACTTCCAGCTGTGTTCTGTGGAGGTCACTTGT
  GAATCAGGCAGTGTCATGGCAGCCACCCTCGCCAACGGTGGGATCTGCCCCATCACAGGCGAGAGTGTG
  CTGAGTGCTGAAGCAGTGCGCAACACCCTCAGCCTCATGCATTCCTGCGGCATGTATGACTTCTCTGGC
  CAGTTTGCCTTCCACGTGGGCCTGCCAGCCAAGTCAGCTGTATCAGGAGCCATCCTCCTGGTGGTACCC
  AATGTCATGGGAATGATGTGCCTGTCACCCCCATTGGACAAGCTGGGGAACAGCCATAGGGGGACCAGC
  TTCTGCCAGAAGTTGGTGTCTCTCTTCAATTTCCACAACTATGACAACCTGAGGCACTGTGCTCGGAAG
  TTAGACCCACGGCGTGAAGGGGCAGAAATTCGGAACAAGACTGTGGTCAACCTGTTATTTGCTGCCTAT
  AGTGGCGATGTCTCAGCTCTTCGAAGGTTTGCCTTGTCAGCCATGGATATGGAACAGAAAGACTATGAC
  TCGCGCACAGCTCTGCATGTTGCTGCAGCTGAAGGACACATCGAAGTTGTTAAATTCCTGATCGAGGCT
  TGCAAAGTGAATCCTTTTGCCAAGGACAGGTGGGGCAACATTCCCCTGGATGATGCTGTGCAGTTCAAC
  CATCTGGAGGTGGTCAAACTGCTTCAAGATTACCAGGACTCCTACACACTCTCTGAAACTCAGGCTGAG
  GCAGCAGCTGAGGCCCTGTCCAAAGAGAACTTAGAAAGCATGGTA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237542
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCF
  PKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSG
  QFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARK
  LDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEA
  CKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001280798
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001280798.1, NP_001267727.1
• RefSeq Size: 2296 bp
• RefSeq ORF: 1014 bp
• Locus ID: 27165
• Cytogenetics: 12q13.3
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism
• MW: 37.5 kDa
• Gene Summary: The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]