TXNRD2 (NM_001282512) Human Tagged ORF Clone

CAT#: RG237559

TXNRD2 (GFP-tagged) - Human thioredoxin reductase 2 (TXNRD2), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TXNRD2
• Synonyms: GCCD5; SELZ; TR; TR-BETA; TR3; TRXR2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237559 representing NM_001282512.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCAATGGCGGTGGCGCTGCGGGGATTAGGAGGGCGCTTCCGGTGGCGGACGCAGGCCGTGGCG
  GGCGGGGTGCGGGGCGCGGCGCGGGGCGCAGCAGCAGGTCAGCGGGACTATGATCTCCTGGTGGTCGGC
  GGGGGATCTGGTGGCCTGGCTTGTGCCAAGGAGGCCGCCCAGCTGGGAAGGAAGGTGGCCGTGGTGGAC
  TACGTGGAACCTTCTCCCCAAGGCACCCGGTGGGGCCTCGGCGGCACCTGCGTCAACGTGGGCTGCATC
  CCCAAGAAGCTGATGCACCAGGCGGCACTGCTGGGAGGCCTGATCCAAGATGCCCCCAACTATGGCTGG
  GAGGTGGCCCAGCCCGTGCCGCATGACTGGAGGAAGATGGCAGAAGCTGTTCAAAATCACGTGAAATCC
  TTGAACTGGGGCCACCGTGTCCAGCTTCAGGACAGAAAAGTCAAGTACTTTAACATCAAAGCCAGCTTT
  GTTGACGAGCACACGGTTTGCGGCGTTGCCAAAGGTGGGAAAGAGATTCTGCTGTCAGCCGATCACATC
  ATCATTGCTACTGGAGGGCGGCCGAGATACCCCACGCACATCGAAGGTGCCTTGGAATATGGAATCACA
  AGTGATGACATCTTCTGGCTGAAGGAATCCCCTGGAAAAACGTTGGTGGTCGGGGCCAGCTATGTGGCC
  CTGGAGTGTGCTGGCTTCCTCACCGGGATTGGGCTGGACACCACCATCATGATGCGCAGCATCCCCCTC
  CGCGGCTTCGACCAGCAAATGTCCTCCATGGTCATAGAGCACATGGCATCTCATGGCACCCGGTTCCTG
  AGGGGCTGTGCCCCCTCGCGGGTCAGGAGGCTCCCTGATGGCCAGCTGCAGGTCACCTGGGAGGACAGC
  ACCACCGGCAAGGAGGACACGGGCACCTTTGACACCGTCCTGTGGGCCATAGCACCTTGCATCTCTGCG
  TGTCTCCCCACCACCGTGGGACATGCTGGAAAAAACCAGAGAAGAGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237559
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAAMAVALRGLGGRFRWRTQAVAGGVRGAARGAAAGQRDYDLLVVGGGSGGLACAKEAAQLGRKVAVVD
  YVEPSPQGTRWGLGGTCVNVGCIPKKLMHQAALLGGLIQDAPNYGWEVAQPVPHDWRKMAEAVQNHVKS
  LNWGHRVQLQDRKVKYFNIKASFVDEHTVCGVAKGGKEILLSADHIIIATGGRPRYPTHIEGALEYGIT
  SDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRFL
  RGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIAPCISACLPTTVGHAGKNQRRD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282512
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282512.3
• RefSeq Size: 2081 bp
• RefSeq ORF: 1017 bp
• Locus ID: 10587
• Cytogenetics: 22q11.21
• Protein Families: Druggable Genome
• Protein Pathways: Pyrimidine metabolism
• MW: 36.8 kDa
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene. [provided by RefSeq, Jun 2017]