RPP40 (NM_001286132) Human Tagged ORF Clone

CAT#: RG237574

RPP40 (GFP-tagged) - Human ribonuclease P/MRP 40kDa subunit (RPP40), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: RPP40
• Synonyms: bA428J1.3; RNASEP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237574 representing NM_001286132.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCACGCTGCGCCGGCTTCGGGAGGCGCCGCGGCACTTACTGGTTTGCGAGAAATCCAACTTCGGC
  AACCACAAGTCGCGCCACCGGCATCTTGTGCAGACGCACTACTATAACTACAGGGTTTCATTTCTCATT
  CCTGAATGTGGGATACTATCGGAAGAACTGAAAAACCTGGTCATGAACACTGGACCCTATTACTTTGTG
  AAGAATTTACCTCTTCATGAATTAATTACACCTGAATTCATCAGTACCTTTATAAAGAAAGGGAAATTA
  ATTTTGTCACTGGATAAAGACACTTATGAAGAAACTGGACTTCAGGGTCATCCATCTCAGTTTTCTGGC
  AGAAAAATTATGAAATTTATTGTTTCCATTGATTTGATGGAATTATCCTTAAACTTGGATTCTAAGAAG
  TATGAAAGAATATCTTGGTCTTTCAAAGAAAAGAAGCCATTGAAATTTGATTTTCTTTTGGCTTGGCAT
  AAAACAGGTTCAGAAGAATCGACAATGATGTCATATTTTTCCAAGTACCAAATTCAGGAGCATCAGCCA
  AAAGTAGCACTGAGCACGTTGAGAGATCTCCAGTGCCCAGTGCTGCAGAGCAGCGAGCTGGAGGGAACG
  CCAGAGGTGTCCTGCCGGGCTCTGGAGCTCTTCGACTGGCTCGGCGCCGTCTTCAGTAATGTCGACCTA
  AATAATGAGCCTAATAATTTCATATCAACCTATTGCTGTCCTGAGCCAAGCACAGTGGTGGCAAAAGCT
  TATTTGTGTACAATCACTGGCTTCATACTTCCAGAGAAGATCTGTCTCCTATTGGAACATCTCTGTCAC
  TACTTTGATGAACCGAAGTTAGCTCCATGGGTTACACTGTCCGTTCAAGGCTTTGCAGACAGCCCTGTT
  TCTTGGGAAAAAAATGAACATGGTTTTCGAAAAGGAGGAGAACATTTATATAACTTTGTGATTTTTAAT
  AATCAGGACTATTGGCTTCAGATGGCTGTTGGGGCAAATGATCACTGTCCACCA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237574
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MATLRRLREAPRHLLVCEKSNFGNHKSRHRHLVQTHYYNYRVSFLIPECGILSEELKNLVMNTGPYYFV
  KNLPLHELITPEFISTFIKKGKLILSLDKDTYEETGLQGHPSQFSGRKIMKFIVSIDLMELSLNLDSKK
  YERISWSFKEKKPLKFDFLLAWHKTGSEESTMMSYFSKYQIQEHQPKVALSTLRDLQCPVLQSSELEGT
  PEVSCRALELFDWLGAVFSNVDLNNEPNNFISTYCCPEPSTVVAKAYLCTITGFILPEKICLLLEHLCH
  YFDEPKLAPWVTLSVQGFADSPVSWEKNEHGFRKGGEHLYNFVIFNNQDYWLQMAVGANDHCPP
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286132
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286132.2
• RefSeq Size: 1131 bp
• RefSeq ORF: 1023 bp
• Locus ID: 10799
• Cytogenetics: 6p25.1
• Protein Families: Stem cell - Pluripotency
• MW: 39.8 kDa
• Gene Summary: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. [UniProtKB/Swiss-Prot Function]