USP46 (NM_001286767) Human Tagged ORF Clone

CAT#: RG237679

USP46 (GFP-tagged) - Human ubiquitin specific peptidase 46 (USP46), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: USP46
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237679 representing NM_001286767.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACTGTCCGAAACATCGCCTCCATCTGTAATATGGGCACCAATGCCTCTGCTCTGGAAAAAGACATT
  GGTCCAGAGCAGTTTCCAATCAATGAACACTATTTCGGATTGGTCAATGCATTGTACTTCTGCCGTCCA
  TTCCGGGAGAATGTGTTGGCATACAAGGCCCAGCAAAAGAAGAAGGAAAACTTGCTGACGTGCCTGGCG
  GACCTTTTCCACAGCATTGCCACACAGAAGAAGAAGGTTGGCGTCATCCCACCAAAGAAGTTCATTTCA
  AGGCTGAGAAAAGAGAATGATCTCTTTGATAACTACATGCAGCAGGATGCTCATGAATTTTTAAATTAT
  TTGCTAAACACTATTGCGGACATCCTTCAGGAGGAGAAGAAACAGGAAAAACAAAATGGAAAATTAAAA
  AATGGCAACATGAACGAACCTGCGGAAAATAATAAACCAGAACTCACCTGGGTCCATGAGATTTTTCAG
  GGAACGCTTACCAATGAAACTCGATGCTTGAACTGTGAAACTGTTAGTAGCAAAGATGAAGATTTTCTT
  GACCTTTCTGTTGATGTGGAGCAGAATACATCCATTACCCACTGTCTAAGAGACTTCAGCAACACAGAA
  ACACTGTGTAGTGAACAAAAATATTATTGTGAAACATGCTGCAGCAAACAAGAAGCCCAGAAAAGGATG
  AGGGTAAAAAAGCTGCCCATGATCTTGGCCCTGCACCTAAAGCGGTTCAAGTACATGGAGCAGCTGCAC
  AGATACACCAAGCTGTCTTACCGTGTGGTCTTCCCTCTGGAACTCCGGCTCTTCAACACCTCCAGTGAT
  GCAGTGAACCTGGACCGCATGTATGACTTGGTTGCGGTGGTCGTTCACTGTGGCAGTGGTCCTAATCGT
  GGGCATTATATCACTATTGTGAAAAGTCACGGCTTCTGGCTTTTGTTTGATGATGACATTGTAGAGAAA
  ATAGATGCTCAAGCTATTGAAGAATTCTATGGCCTGACGTCAGATATATCAAAAAATTCAGAATCTGGA
  TATATTTTATTCTATCAGTCAAGAGAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237679
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNALYFCRPFRENVLAYKAQQKKKENLLTCLA
  DLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLK
  NGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTE
  TLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSSD
  AVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESG
  YILFYQSRE
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286767
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286767.2
• RefSeq Size: 7930 bp
• RefSeq ORF: 1065 bp
• Locus ID: 64854
• Cytogenetics: 4q12
• Protein Families: Druggable Genome, Protease
• MW: 41.6 kDa
• Gene Summary: Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]). [supplied by OMIM, Jun 2009]