HARS2 (NM_001278732) Human Tagged ORF Clone

CAT#: RG237728

HARS2 (GFP-tagged) - Human histidyl-tRNA synthetase 2, mitochondrial (HARS2), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: HARS2
• Synonyms: HARSL; HARSR; HisRS; HO3; PRLTS2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237728 representing NM_001278732.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTTGTGAGGGAGAAAATTCTTGATTTGGTTATCAGCTGCTTTAAACGTCATGGAGCAAAGGGGATG
  GACACCCCAGCATTTGAGCTGAAGGATTTTGACATTGCTGGTCAGTTTGACCCTATGATCCCCGATGCA
  GAGTGTTTGAAGATCATGTGTGAAATCCTAAGTGGATTGCAGTTGGGAGACTTTCTCATTAAGGTAAAT
  GACCGGCGGATTGTGGATGGGATGTTTGCTGTCTGTGGTGTTCCTGAAAGCAAGTTCCGTGCCATCTGC
  TCCTCCATAGATAAACTAGACAAGATGGCTTGGAAAGATGTGAGACATGAGATGGTGGTGAAGAAAGGC
  CTGGCTCCTGAGGTGGCTGATCGAATTGGGGACTATGTCCAGTGTCATGGTGGGGTATCCCTAGTAGAG
  CAAATGTTTCAGGATCCCAGACTATCCCAGAACAAGCAGGCCCTGGAGGGCCTGGGAGACCTAAAGCTG
  CTATTTGAATACCTGACTTTATTTGGAATTGCTGATAAGATCTCCTTTGACCTCAGCCTGGCTCGGGGC
  CTAGACTACTATACAGGAGTGATCTATGAAGCAGTGCTGCTGCAGACCCCAACTCAGGCTGGGGAGGAG
  CCCCTGAATGTGGGCAGTGTGGCTGCTGGTGGGCGCTATGATGGGCTGGTGGGCATGTTTGACCCCAAG
  GGCCACAAGGTGCCATGTGTGGGACTCAGCATTGGGGTTGAGCGAATCTTCTACATTGTGGAGCAGAGG
  ATGAAGACCAAAGGTGAGAAGGTGCGGACTACAGAGACTCAAGTGTTTGTGGCCACACCACAGAAGAAC
  TTTCTCCAAGAACGGTTGAAGCTTATTGCAGAGCTTTGGGATTCTGGAATCAAGGCAGAGATGCTATAC
  AAGAACAACCCCAAACTATTAACCCAGCTGCACTATTGTGAGAGCACAGGCATTCCACTGGTGGTCATT
  ATTGGTGAGCAAGAACTGAAAGAAGGGGTCATCAAGATCCGTTCAGTGGCCAGCAGAGAGGAGGTGGCC
  ATTAAACGGGAAAATTTTGTGGCTGAAATTCAGAAGCGACTGTCTGAGTCT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237728
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVVREKILDLVISCFKRHGAKGMDTPAFELKDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVN
  DRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVE
  QMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEE
  PLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKN
  FLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVA
  IKRENFVAEIQKRLSES
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278732
• ORF Size: 1086 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278732.2
• RefSeq Size: 2393 bp
• RefSeq ORF: 1089 bp
• Locus ID: 23438
• Cytogenetics: 5q31.3
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 40.9 kDa
• Gene Summary: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]