NPSR1 (NM_001300933) Human Tagged ORF Clone

CAT#: RG237759

NPSR1 (GFP-tagged) - Human neuropeptide S receptor 1 (NPSR1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: NPSR1
• Synonyms: ASRT2; GPR154; GPRA; NPSR; PGR14; VRR1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237759 representing NM_001300933.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCAGCCAACTTCACAGAGGGCAGCTTCGATTCCAGTGGGACCGGGCAGACGCTGGATTCTTCCCCA
  GTGGCTTGCACTGAAACAGTGACTTTTACTGAAGTGGTGGAAGGAAAGGAATGGGGTTCCTTCTACTAC
  TCCTTTAAGACTGAGCAATTGATAACTCTGTGGGTCCTCTTTGTTTTTACCATTGTTGGAAACTCCGTT
  GTGCTTTTTTCCACATGGAGGAGAAAGAAGAAGTCAAGAATGACCTTCTTTGTGACTCAGCTGGCCATC
  ACAGATATTAATTGGCGATTCACTGGAGACTTCACGGCACCTGACCTGGTTTGCCGAGTGGTCCGCTAT
  TTGCAGGTTGTGCTGCTCTACGCCTCTACCTACGTCCTGGTGTCCCTCAGCATAGACAGATACCATGCC
  ATCGTCTACCCCATGAAGTTCCTTCAAGGAGAAAAGCAAGCCAGGGTCCTCATTGTGATCGCCTGGAGC
  CTGTCTTTTCTGTTCTCCATTCCCACCCTGATCATATTTGGGAAGAGGACACTGTCCAACGGTGAAGTG
  CAGTGCTGGGCCCTGTGGCCTGACGACTCCTACTGGACCCCATACATGACCATCGTGGCCTTCCTGGTG
  TACTTCATCCCTCTGACAATCATCAGCATCATGTATGGCATTGTGATCCGAACTATTTGGATTAAAAGC
  AAAACCTACGAAACAGTGATTTCCAACTGCTCAGATGGGAAACTGTGCAGCAGCTATAACCGAGGACTC
  ATCTCAAAGGCAAAAATCAAGGCTATCAAGTATAGCATCATCATCATTCTTGCCTTCATCTGCTGTTGG
  AGTCCATACTTCCTGTTTGACATTTTGGACAATTTCAACCTCCTTCCAGACACCCAGGAGCGTTTCTAT
  GCCTCTGTGATCATTCAGAACCTGCCAGCATTGAATAGTGCCATCAACCCCCTCATCTACTGTGTCTTC
  AGCAGCTCCATCTCTTTCCCCTGCAGGGTCATCCGTCTCCGTCAGCTCCAGGAGGCTGCGCTAATGCTC
  TGCCCTCAACGAGAGAACTGGAAGGGTACTTGGCCAGGTGTACCTTCCTGGGCTCTTCCAAGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237759
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPANFTEGSFDSSGTGQTLDSSPVACTETVTFTEVVEGKEWGSFYYSFKTEQLITLWVLFVFTIVGNSV
  VLFSTWRRKKKSRMTFFVTQLAITDINWRFTGDFTAPDLVCRVVRYLQVVLLYASTYVLVSLSIDRYHA
  IVYPMKFLQGEKQARVLIVIAWSLSFLFSIPTLIIFGKRTLSNGEVQCWALWPDDSYWTPYMTIVAFLV
  YFIPLTIISIMYGIVIRTIWIKSKTYETVISNCSDGKLCSSYNRGLISKAKIKAIKYSIIIILAFICCW
  SPYFLFDILDNFNLLPDTQERFYASVIIQNLPALNSAINPLIYCVFSSSISFPCRVIRLRQLQEAALML
  CPQRENWKGTWPGVPSWALPR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300933
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001300933.2
• RefSeq Size: 1423 bp
• RefSeq ORF: 1101 bp
• Locus ID: 387129
• Cytogenetics: 7p14.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 42.4 kDa
• Gene Summary: This gene encodes a member of the vasopressin/oxytocin subfamily of G protein-coupled receptors. The encoded membrane protein acts as a receptor for neuropeptide S and affects a variety of cellular processes through its signaling. Increased expression of this gene in ciliated cells of the respiratory epithelium and in bronchial smooth muscle cells is associated with asthma. Polymorphisms in this gene have also been associated with asthma susceptibility, panic disorders, inflammatory bowel disease, and rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]