SUV420h1 (KMT5B) (NM_001300909) Human Tagged ORF Clone

CAT#: RG237783

SUV420H1 (GFP-tagged) - Human suppressor of variegation 4-20 homolog 1 (Drosophila) (SUV420H1), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: KMT5B
• Synonyms: CGI-85; CGI85; MRD51; SUV420H1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237783 representing NM_001300909.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGTGGTTGGGAGAATCCAAGAACATGGTGGTGAATGGCAGGAGAAATGGAGGCAAGTTGTCTAAT
  GACCATCAGCAGAATCAATCAAAATTACAGCACACGGGGAAGGACACCCTGAAGGCTGGCAAAAATGCA
  GTCGAGAGGAGGTCGAACAGATGTAATGGTAACTCGGGATTTGAAGGACAGAGTCGCTATGTACCATCC
  TCTGGAATGTCCGCCAAGGAACTCTGTGAAAATGATGACCTAGCAACCAGTTTGGTTCTTGATCCCTAT
  TTAGGTTTTCAAACACACAAAATGAATACTAGATTTAGGCCTATTAAAGGAAGGCAGGAAGAACTAAAG
  GAAGTAATTGAACGTTTTAAGAAAGATGAACACTTGGAGAAAGCCTTCAAATGTTTGACTTCAGGCGAA
  TGGGCACGGCACTATTTTCTCAACAAGAATAAAATGCAGGAGAAATTATTCAAAGAACATGTATTTATT
  TATTTGCGAATGTTTGCAACTGACAGTGGATTTGAAATATTGCCATGTAATAGATACTCATCAGAACAA
  AATGGAGCCAAAATAGTTGCAACAAAAGAGTGGAAACGAAATGACAAAATAGAATTACTGGTGGGTTGT
  ATTGCCGAACTTTCAGAAATTGAGGAGAACATGCTACTTAGACATGGAGAAAACGACTTCAGTGTCATG
  TACTCCACAAGGAAAAACTGTGCTCAACTCTGGCTGGGTCCTGCTGCGTTTATAAACCATGATTGCAGA
  CCTAATTGTAAGTTTGTGTCAACTGGTCGAGATACAGCATGTGTGAAGGCTCTAAGAGACATTGAACCT
  GGAGAAGAAATTTCTTGTTATTATGGAGATGGGTTCTTTGGAGAAAATAATGAGTTCTGCGAGTGTTAC
  ACTTGCGAAAGACGGGGCACTGGTGCTTTTAAATCCAGAGTGGGACTGCCTGCGCCTGCTCCTGTTATC
  AATAGCAAATATGGACTCAGAGAAACAGATAAACGTTTAAATAGGCTTAAAAAGTTAGGTGACAGCAGC
  AAAAATTCAGACAGTCAATCTGTCAGCTCTAACACTGATGCAGATACCACTCAGGAAAAAAACAATGCA
  AGTAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237783
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKWLGESKNMVVNGRRNGGKLSNDHQQNQSKLQHTGKDTLKAGKNAVERRSNRCNGNSGFEGQSRYVPS
  SGMSAKELCENDDLATSLVLDPYLGFQTHKMNTRFRPIKGRQEELKEVIERFKKDEHLEKAFKCLTSGE
  WARHYFLNKNKMQEKLFKEHVFIYLRMFATDSGFEILPCNRYSSEQNGAKIVATKEWKRNDKIELLVGC
  IAELSEIEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEP
  GEEISCYYGDGFFGENNEFCECYTCERRGTGAFKSRVGLPAPAPVINSKYGLRETDKRLNRLKKLGDSS
  KNSDSQSVSSNTDADTTQEKNNASK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300909
• ORF Size: 1110 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001300909.2
• RefSeq Size: 2758 bp
• RefSeq ORF: 1113 bp
• Locus ID: 51111
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• MW: 42.5 kDa
• Gene Summary: This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]