G2A (GPR132) (NM_001278695) Human Tagged ORF Clone
CAT#: RG237794
- TrueORF®
GPR132 (GFP-tagged) - Human G protein-coupled receptor 132 (GPR132), transcript variant 3
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | GPR132 |
Synonyms | G2A |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG237794 representing NM_001278695.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCAGGAAACGCCACCCCAGTGACCACCACTGCCCCGTGGGCCTCCCTGGGCCTCTCCGCCAAGACC TGCAACAACGTGTCCTTCGAAGAGAGCAGGATAGTCCTGGTCGTGGTGTACAGCGCGGTGTGCACGCTG GGGGTGCCGGCCAACTGCCTGACTGCGTGGCTGGCGCTGCTGCAGGTACTGCAGGGCAACGTGCTGGCC GTCTACCTGCTCTGCCTGGCACTCTGCGAGCTGCTGTACACAGGCACGCTGCCACTCTGGGTCATCTAT ATCCGCAACCAGCACCGCTGGACCCTAGGCCTGCTGGCCTGCAAGGTGACCGCCTACATCTTCTTCTGC AACATCTACGTCAGCATCCTCTTCCTGTGCTGCATCTCCTGCGACCGCTTCGTGGCCGTGGTGTACGCG CTGGAGAGTCGGGGCCGCCGCCGCCGGAGGACCGCCATCCTCATCTCCGCCTGCATCTTCATCCTCGTC GGGATCGTTCACTACCCGGTGTTCCAGACGGAAGACAAGGAGACCTGCTTTGACATGCTGCAGATGGAC AGCAGGATTGCCGGGTACTACTACGCCAGGTTCACCGTTGGCTTTGCCATCCCTCTCTCCATCATCGCC TTCACCAACCACCGGATTTTCAGGAGCATCAAGCAGAGCATGGGCTTAAGCGCTGCCCAGAAGGCCAAG GTGAAGCACTCGGCCATCGCGGTGGTTGTCATCTTCCTAGTCTGCTTCGCCCCGTACCACCTGGTTCTC CTCGTCAAAGCCGCTGCCTTTTCCTACTACAGAGGAGACAGGAACGCCATGTGCGGCTTGGAGGAAAGG CTGTACACAGCCTCTGTGGTGTTTCTGTGCCTGTCCACGGTGAACGGCGTGGCTGACCCCATTATCTAC GTGCTGGCCACGGACCATTCCCGCCAAGAAGTGTCCAGAATCCATAAGGGGTGGAAAGAGTGGTCCATG AAGACAGACGTCACCAGGCTCACCCACAGCAGGGACACCGAGGAGCTGCAGTCGCCCGTGGCCCTTGCA GACCACTACACCTTCTCCAGGCCCGTGCACCCACCAGGGTCACCATGCCCTGCAAAGAGGCTGATTGAG GAGTCCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG237794
Blue=ORF Red=Cloning site Green=Tag(s) MPGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPANCLTAWLALLQVLQGNVLA VYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAYIFFCNIYVSILFLCCISCDRFVAVVYA LESRGRRRRRTAILISACIFILVGIVHYPVFQTEDKETCFDMLQMDSRIAGYYYARFTVGFAIPLSIIA FTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEER LYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALA DHYTFSRPVHPPGSPCPAKRLIEESC TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001278695 |
ORF Size | 1113 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001278695.2 |
RefSeq Size | 3008 bp |
RefSeq ORF | 1116 bp |
Locus ID | 29933 |
Cytogenetics | 14q32.33 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
MW | 41.9 kDa |
Gene Summary | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
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