G2A (GPR132) (NM_001278695) Human Tagged ORF Clone

CAT#: RG237794

GPR132 (GFP-tagged) - Human G protein-coupled receptor 132 (GPR132), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: GPR132
• Synonyms: G2A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237794 representing NM_001278695.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCAGGAAACGCCACCCCAGTGACCACCACTGCCCCGTGGGCCTCCCTGGGCCTCTCCGCCAAGACC
  TGCAACAACGTGTCCTTCGAAGAGAGCAGGATAGTCCTGGTCGTGGTGTACAGCGCGGTGTGCACGCTG
  GGGGTGCCGGCCAACTGCCTGACTGCGTGGCTGGCGCTGCTGCAGGTACTGCAGGGCAACGTGCTGGCC
  GTCTACCTGCTCTGCCTGGCACTCTGCGAGCTGCTGTACACAGGCACGCTGCCACTCTGGGTCATCTAT
  ATCCGCAACCAGCACCGCTGGACCCTAGGCCTGCTGGCCTGCAAGGTGACCGCCTACATCTTCTTCTGC
  AACATCTACGTCAGCATCCTCTTCCTGTGCTGCATCTCCTGCGACCGCTTCGTGGCCGTGGTGTACGCG
  CTGGAGAGTCGGGGCCGCCGCCGCCGGAGGACCGCCATCCTCATCTCCGCCTGCATCTTCATCCTCGTC
  GGGATCGTTCACTACCCGGTGTTCCAGACGGAAGACAAGGAGACCTGCTTTGACATGCTGCAGATGGAC
  AGCAGGATTGCCGGGTACTACTACGCCAGGTTCACCGTTGGCTTTGCCATCCCTCTCTCCATCATCGCC
  TTCACCAACCACCGGATTTTCAGGAGCATCAAGCAGAGCATGGGCTTAAGCGCTGCCCAGAAGGCCAAG
  GTGAAGCACTCGGCCATCGCGGTGGTTGTCATCTTCCTAGTCTGCTTCGCCCCGTACCACCTGGTTCTC
  CTCGTCAAAGCCGCTGCCTTTTCCTACTACAGAGGAGACAGGAACGCCATGTGCGGCTTGGAGGAAAGG
  CTGTACACAGCCTCTGTGGTGTTTCTGTGCCTGTCCACGGTGAACGGCGTGGCTGACCCCATTATCTAC
  GTGCTGGCCACGGACCATTCCCGCCAAGAAGTGTCCAGAATCCATAAGGGGTGGAAAGAGTGGTCCATG
  AAGACAGACGTCACCAGGCTCACCCACAGCAGGGACACCGAGGAGCTGCAGTCGCCCGTGGCCCTTGCA
  GACCACTACACCTTCTCCAGGCCCGTGCACCCACCAGGGTCACCATGCCCTGCAAAGAGGCTGATTGAG
  GAGTCCTGC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237794
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPANCLTAWLALLQVLQGNVLA
  VYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAYIFFCNIYVSILFLCCISCDRFVAVVYA
  LESRGRRRRRTAILISACIFILVGIVHYPVFQTEDKETCFDMLQMDSRIAGYYYARFTVGFAIPLSIIA
  FTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEER
  LYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALA
  DHYTFSRPVHPPGSPCPAKRLIEESC
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278695
• ORF Size: 1113 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278695.2
• RefSeq Size: 3008 bp
• RefSeq ORF: 1116 bp
• Locus ID: 29933
• Cytogenetics: 14q32.33
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 41.9 kDa
• Gene Summary: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]