VPS36 (NM_001282168) Human Tagged ORF Clone

CAT#: RG237843

VPS36 (GFP-tagged) - Human vacuolar protein sorting 36 homolog (S. cerevisiae) (VPS36), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: VPS36
• Synonyms: C13orf9; CGI-145; EAP45
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237843 representing NM_001282168.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACCGCTTCGTTTGGACCAGCGGCCTCCTGGAGATCAACGAGACCCTGGTGATCCAGCAGCGCGGG
  ATAAAATTTGATGCTGGGACTCTCCTTCTTAGTACACACCGACTGATTTGGAGAGATCAGAAAAATCAT
  GAGTGTTGCATGGCCATTCTCCTTTCCCAAATTGTGTTCATTGAAGAACAGGCGGCTGGAATTGGGAAG
  AGTGCCAAAATAGTGGTTCATCTTCACCCAGCTCCTCCTAACAAAGAACCTGGCCCATTCCAGAGTAGT
  AAGAACTCCTACATCAAACTCTCCTTCAAAGAACATGGCCAGATTGAGTTTTACAGGCGTTTATCAGAG
  GAAATGACACAAAGAAGATGGGAGAATATGCCAGTTTCCCAGTCATTACAAACAAATAGAGGACCCCAG
  CCAGGAAGAATAAGGGCTGTAGGAATTGTAGGTATTGAAAGGAAACTGGAAGAAAAAAGAAAAGAAACT
  GACAAAAACATTTCTGAGGCCTTTGAAGACCTCAGCAAACTAATGATCAAGGCTAAGGAAATGGTGGAA
  TTATCAAAATCAATTGCTAATAAAATTAAAGACAAACAAGGTGACATCACAGAAGATGAGACCATCAGG
  TTTAAATCCTACTTGCTGAGCATGGGAATAGCTAACCCAGTTACCAGAGAAACCTACGGCTCAGGCACA
  CAGTACCACATGCAGCTGGCCAAACAACTGGCTGGAATATTGCAGGTGCCTTTAGAGGAACGAGGGGGA
  ATAATGTCACTCACGGAGGTGTACTGCTTAGTAAACCGAGCTCGAGGAATGGAATTGCTCTCACCAGAA
  GATTTAGTGAATGCGTGCAAGATGCTGGAAGCACTGAAATTACCTCTCAGGCTCCGTGTGTTTGACAGT
  GGCGTCATGGTAATTGAGCTTCAGTCTCACAAGGAAGAGGAAATGGTGGCCTCGGCCCTGGAGACAGTT
  TCAGAAAAGGGATCCCTAACATCAGAAGAGTTTGCTAAGCTTGTGGGAATGTCTGTCCTCCTAGCCAAA
  GAAAGGTTGCTGCTTGCAGAGAAGATGGGCCATCTTTGCCGTGATGACTCAGTGGAAGGCCTGCGTTTT
  TACCCAAATTTATTTATGACACAGAGC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237843
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDRFVWTSGLLEINETLVIQQRGIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGK
  SAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQ
  PGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIR
  FKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPE
  DLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAK
  ERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282168
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282168.2
• RefSeq Size: 4446 bp
• RefSeq ORF: 1134 bp
• Locus ID: 51028
• Cytogenetics: 13q14.3
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis
• MW: 43.2 kDa
• Gene Summary: This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013]