ATE1 (NM_001288734) Human Tagged ORF Clone

CAT#: RG238001

ATE1 (GFP-tagged) - Human arginyltransferase 1 (ATE1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ATE1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238001 representing NM_001288734.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATTCCACAATGGATGATGCTGTTGCGGGTGACTTTGCATTGATAAATAAACTGGATATACAGTGT
  GATCTTAAAACACTCAGTGATGACATCAAAGAGAGTTTAGAGAGTGAAGGAAAAAATTCAAAGAAAGAA
  GAACCTCAGGAATTACTTCAGTCACAAGATTTCGTAGGAGAGAAGTTGGGCTCTGGTGAACCGTCACAT
  TCAGTTAAAGTTCACACAGTTCCTAAGCCAGGCAAAGGGGCTGATTTGAGTAAGCCTCCATGTCGAAAA
  GCAAAGGAAATCCGGAAAGAAAGGAAAAGGTTAAAACTAATGCAGCAGAACCCAGCTGGAGAACTTGAG
  GGTTTCCAGGCTCAAGGTCACCCACCATCTTTGTTTCCACCAAAGGCTAAATCCAACCAGCCAAAATCA
  CTCGAAGATTTAATTTTTGAGTCTTTACCAGAGAATGCATCACACAAGTTAGAGGTGAGGGTGGTGAGA
  TCATCTCCACCAAGTTCGCAGTTCAAAGCCACACTTCTGGAGTCTTACCAGGTCTATAAACGTTACCAG
  ATGGTTATTCACAAGAACCCACCTGATACGCCAACCGAAAGCCAGTTCACAAGATTCCTTTGCAGTTCA
  CCCTTGGAGGCAGAGACTCCCCCTAATGGGCCAGATTGTGGCTATGGCTCCTTTCACCAGCAGTACTGG
  CTTGACGGAAAGATCATTGCTGTGGGGGTGATTGACATCCTCCCAAACTGTGTATCATCTGTGTATTTG
  TACTACGATCCTGATTATTCGTTTTTGTCTTTGGGCGTCTACTCTGCACTACGAGAAATTGCTTTTACT
  AGGCAGCTTCATGAGAAAACTTCTCAACTCAGCTATTATTATATGGGTTTCTACATTCATTCATGTCCC
  AAGATGAAATATAAGGGTCAGTATAGACCTTCTGATTTGCTGTGCCCTGAGACATATGTTTGGGTACCC
  ATTGAGCAATGCCTGCCTTCACTTGAAAACTCCAAGTACTGCCGTTTCAACCAGGACCCAGAAGCAGTG
  GATGAGGATCGCAGTACGGAACCTGACCGATTGCAGGTGTTTCACAAGAGAGCCATCATGCCTTACGGT
  GTTTATAAGAAACAGCAGAAAGACCCAAGTGAGGAGGCTGCTGTTCTGCAGTACGCCAGCCTGGTGGGG
  CAGAAGTGCTCCGAGCGGATGCTGCTGTTCAGAAAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238001
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDSTMDDAVAGDFALINKLDIQCDLKTLSDDIKESLESEGKNSKKEEPQELLQSQDFVGEKLGSGEPSH
  SVKVHTVPKPGKGADLSKPPCRKAKEIRKERKRLKLMQQNPAGELEGFQAQGHPPSLFPPKAKSNQPKS
  LEDLIFESLPENASHKLEVRVVRSSPPSSQFKATLLESYQVYKRYQMVIHKNPPDTPTESQFTRFLCSS
  PLEAETPPNGPDCGYGSFHQQYWLDGKIIAVGVIDILPNCVSSVYLYYDPDYSFLSLGVYSALREIAFT
  RQLHEKTSQLSYYYMGFYIHSCPKMKYKGQYRPSDLLCPETYVWVPIEQCLPSLENSKYCRFNQDPEAV
  DEDRSTEPDRLQVFHKRAIMPYGVYKKQQKDPSEEAAVLQYASLVGQKCSERMLLFRN
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288734
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001288734.1, NP_001275663.1
• RefSeq Size: 4826 bp
• RefSeq ORF: 1212 bp
• Locus ID: 11101
• Cytogenetics: 10q26.13
• MW: 46.3 kDa
• Gene Summary: This gene encodes an arginyltransferase, an enzyme that is involved in posttranslational conjugation of arginine to N-terminal aspartate or glutamate residues. Conjugation of arginine to the N-terminal aspartate or glutamate targets proteins for ubiquitin-dependent degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]