ATG4D (NM_001281504) Human Tagged ORF Clone

CAT#: RG238050

ATG4D (GFP-tagged) - Human autophagy related 4D, cysteine peptidase (ATG4D), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ATG4D
• Synonyms: APG4-D; APG4D; AUTL4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238050 representing NM_001281504.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAAGTGCTTCATCTCGCTGGGCGCTGCCCCTACGTCTCCCCAGGTTGGGTGGTTAAAAGCCGGACC
  AGCTTTAGCAAGATCTCCAGCATCCACCTCTGTGGCCGCCGCTACCGTTTCGAGGGCGAGGGTGACATA
  CAGCGTTTCCAGCGGGACTTTGTGTCCCGCCTGTGGCTCACATACCGCCGGGACTTCCCGCCCCTTCCT
  GGGGGCTGCCTGACCTCGGACTGTGGCTGGGGGTGCATGTTACGCAGCGGCCAGATGATGCTGGCACAG
  GGCCTTCTGCTGCATTTCCTGCCCAGAGACTGGACATGGGCCGAGGGCATGGGCCTGGGCCCCCCTGAG
  CTGTCAGGGTCAGCCTCTCCCAGCCGGTACCATGGGCCTGCCCGCTGGATGCCCCCACGCTGGGCCCAG
  GGTGCCCCTGAGCTGGAGCAGGAACGCCGGCACCGGCAGATTGTGTCCTGGTTCGCCGACCACCCCCGG
  GCCCCCTTTGGCCTACACCGGCTGGTGGAGCTTGGGCAGAGCTCAGGCAAGAAGGCAGGTGACTGGTAT
  GGGCCATCGCTAGTGGCACACATCCTCAGGAAAGCCGTGGAGAGCTGCTCCGACGTCACCCGCCTGGTG
  GTGTACGTTTCTCAGGACTGCACAGTGTACAAGGCGGATGTGGCACGCCTGGTGGCCAGGCCAGACCCC
  ACAGCCGAGTGGAAGTCTGTGGTCATCCTGGTGCCCGTGCGACTGGGTGGCGAGACTCTCAACCCCGTG
  TATGTGCCCTGCGTGAAGGAACTCCTGCGTTGCGAGCTGTGCCTGGGCATCATGGGTGGGAAACCGCGA
  CACTCACTGTACTTCATTGGCTACCAAGATGACTTCCTGCTGTACCTGGACCCTCACTACTGCCAGCCC
  ACTGTGGATGTCAGCCAGGCCGACTTCCCCCTGGAGTCCTTCCACTGCACCTCGCCCCGCAAGATGGCC
  TTTGCCAAGATGGACCCAAGCTGTACCGTGGGCTTCTATGCTGGAGACAGGAAGGAGTTTGAGACACTC
  TGCTCAGAGCTGACCAGGGTCCTCAGCTCCTCCTCAGCCACAGAGCGGTACCCCATGTTCACCCTGGCC
  GAGGGCCATGCTCAGGACCACAGCCTGGACGACCTCTGCTCCCAGCTCGCCCAGCCCACACTCCGGCTC
  CCTCGCACAGGGCGGCTCCTCAGGGCCAAACGCCCCAGCTCTGAGGACTTTGTGTTTTTA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238050
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MQVLHLAGRCPYVSPGWVVKSRTSFSKISSIHLCGRRYRFEGEGDIQRFQRDFVSRLWLTYRRDFPPLP
  GGCLTSDCGWGCMLRSGQMMLAQGLLLHFLPRDWTWAEGMGLGPPELSGSASPSRYHGPARWMPPRWAQ
  GAPELEQERRHRQIVSWFADHPRAPFGLHRLVELGQSSGKKAGDWYGPSLVAHILRKAVESCSDVTRLV
  VYVSQDCTVYKADVARLVARPDPTAEWKSVVILVPVRLGGETLNPVYVPCVKELLRCELCLGIMGGKPR
  HSLYFIGYQDDFLLYLDPHYCQPTVDVSQADFPLESFHCTSPRKMAFAKMDPSCTVGFYAGDRKEFETL
  CSELTRVLSSSSATERYPMFTLAEGHAQDHSLDDLCSQLAQPTLRLPRTGRLLRAKRPSSEDFVFL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281504
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001281504.2
• RefSeq Size: 2070 bp
• RefSeq ORF: 1236 bp
• Locus ID: 84971
• Cytogenetics: 19p13.2
• Protein Pathways: Regulation of autophagy
• MW: 46.9 kDa
• Gene Summary: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]