WSB2 (NM_001278557) Human Tagged ORF Clone

CAT#: RG238123

WSB2 (GFP-tagged) - Human WD repeat and SOCS box containing 2 (WSB2), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: WSB2
• Synonyms: SBA2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238123 representing NM_001278557.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGGGTAGACAGGGAGAGCAGGTTTCTCAGGGGGACGGGGACAGGCGAGGCTGTCGCCGTAGAGGAA
  CCGCTGCTGCTGGCCGAACTCAAGCCCGGGCGCCCCCACCAGTTTGATTGGAAGTCCAGCTGTGAAACC
  TGGAGCGTCGCCTTCTCCCCAGATGGCTCCTGGTTTGCTTGGTCTCAAGGACACTGCATCGTCAAACTG
  ATCCCCTGGCCGTTGGAGGAGCAGTTCATCCCTAAAGGGTTTGAAGCCAAAAGCCGAAGTAGCAAAAAT
  GAGACGAAAGGGCGGGGCAGCCCAAAAGAGAAGACGCTGGACTGTGGTCAGATTGTCTGGGGGCTGGCC
  TTCAGCCCGTGGCCTTCCCCACCCAGCAGGAAGCTCTGGGCACGCCACCACCCCCAAGTGCCCGATGTC
  TCTTGCCTGGTTCTTGCTACGGGACTCAACGATGGGCAGATCAAGATCTGGGAGGTGCAGACAGGGCTC
  CTGCTTTTGAATCTTTCCGGCCACCAAGATGTCGTGAGAGATCTGAGCTTCACACCCAGTGGCAGTTTG
  ATTTTGGTCTCCGCGTCACGGGATAAGACTCTTCGCATCTGGGACCTGAATAAACACGGTAAACAGATT
  CAAGTGTTATCGGGCCACCTGCAGTGGGTTTACTGCTGTTCCATCTCCCCAGACTGCAGCATGCTGTGC
  TCTGCAGCTGGAGAGAAGTCGGTCTTTCTATGGAGCATGAGGTCCTACACGTTAATTCGGAAGCTAGAG
  GGCCATCAAAGCAGTGTTGTCTCTTGTGACTTCTCCCCCGACTCTGCCCTGCTTGTCACGGCTTCTTAC
  GATACCAATGTGATTATGTGGGACCCCTACACCGGCGAAAGGCTGAGGTCACTCCACCACACCCAGGTT
  GACCCCGCCATGGATGACAGTGACGTCCACATTAGCTCACTGAGATCTGTGTGCTTCTCTCCAGAAGGC
  TTGTACCTTGCCACGGTGGCAGATGACAGACTCCTCAGGATCTGGGCCCTGGAACTGAAAACTCCCATT
  GCATTTGCTCCTATGACCAATGGGCTTTGCTGCACATTTTTTCCACATGGTGGAGTCATTGCCACAGGG
  ACAAGAGATGGCCACGTCCAGTTCTGGACAGCTCCTAGGGTCCTGTCCTCACTGAAGCACTTATGCCGG
  AAAGCCCTTCGAAGTTTCCTAACAACTTACCAAGTCCTAGCACTGCCAATCCCCAAGAAAATGAAAGAG
  TTCCTCACATACAGGACTTTT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238123
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRVDRESRFLRGTGTGEAVAVEEPLLLAELKPGRPHQFDWKSSCETWSVAFSPDGSWFAWSQGHCIVKL
  IPWPLEEQFIPKGFEAKSRSSKNETKGRGSPKEKTLDCGQIVWGLAFSPWPSPPSRKLWARHHPQVPDV
  SCLVLATGLNDGQIKIWEVQTGLLLLNLSGHQDVVRDLSFTPSGSLILVSASRDKTLRIWDLNKHGKQI
  QVLSGHLQWVYCCSISPDCSMLCSAAGEKSVFLWSMRSYTLIRKLEGHQSSVVSCDFSPDSALLVTASY
  DTNVIMWDPYTGERLRSLHHTQVDPAMDDSDVHISSLRSVCFSPEGLYLATVADDRLLRIWALELKTPI
  AFAPMTNGLCCTFFPHGGVIATGTRDGHVQFWTAPRVLSSLKHLCRKALRSFLTTYQVLALPIPKKMKE
  FLTYRTF
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278557
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278557.1, NP_001265486.1
• RefSeq Size: 2798 bp
• RefSeq ORF: 1266 bp
• Locus ID: 55884
• Cytogenetics: 12q24.23
• Protein Families: Druggable Genome
• MW: 47.6 kDa
• Gene Summary: This gene encodes a member of the WD-protein subfamily. The encoded protein contains five WD-repeats spanning most of the protein and an SOCS box in the C-terminus. The SOCS box may act as a bridge between specific substrate-binding domains and E3 ubiquitin protein ligases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]