VPS45A (VPS45) (NM_001279355) Human Tagged ORF Clone
CAT#: RG238285
- TrueORF®
VPS45 (GFP-tagged) - Human vacuolar protein sorting 45 homolog (S. cerevisiae) (VPS45), transcript variant 4
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | VPS45 |
Synonyms | H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A; VSP45; VSP45A |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG238285 representing NM_001279355.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACGTGGTTTTTGCTGTGAAGCAGTACCCACATTTGTTTTCCCTCAATATTTTGGGTTGCTGCCAG GGTCGAAATTGGGATCCAGCCCAGCTATCTAGAACAACTCAAGGGCTTACAGCTCTCCTTTTATCTCTG AAGAAGTGTCCCATGATTCGTTATCAGCTCTCATCAGAGGCAGCAAAGAGACTTGCAGAGTGCGTTAAG CAAGTGATAACTAAAGAATATGAACTGTTTGAATTCCGTCGGACAGAGGTTCCTCCATTGCTCCTTATT TTAGATCGCTGTGATGATGCCATCACCCCATTGCTAAACCAGTGGACATATCAGGCCATGGTCCACGAA CTACTAGGCATAAACAACAATCGGATTGATCTTTCCAGAGTGCCGGGAATCAGTAAAGACTTAAGAGAA GTGGTCCTATCTGCTGAAAATGATGAATTCTATGCTAATAATATGTACCTGAACTTTGCTGAGATTGGT AGCAATATAAAGAATCTCATGGAAGATTTTCAGAAGAAGAAACCAAAAGAACAGCAAAAACTAGAATCA ATAGCAGACATGAAGGCGTTTGTTGAGAATTATCCACAGTTCAAGAAAATGTCTGGGACTGTTTCAAAG CATGTGACAGTGGTTGGAGAACTGTCTCGATTGGTCAGTGAACGGAATCTGCTGGAGGTTTCAGAGGTT GAGCAAGAACTGGCCTGTCAAAATGACCATTCTAGTGCTCTCCAGAATATAAAAAGGCTTCTGCAGAAC CCCAAAGTGACAGAGTTTGATGCTGCCCGCCTGGTGATGCTTTATGCTTTACATTATGAGCGACACAGC AGCAATAGCCTGCCAGGACTAATGATGGACCTCAGGAATAAAGGTGTTTCTGAGAAGTATCGAAAGCTC GTGTCTGCAGTTGTTGAATATGGTGGTAAACGAGTCAGAGGAAGTGACCTCTTCAGCCCCAAAGATGCT GTGGCTATCACCAAACAATTCCTCAAAGGACTGAAGGGAGTAGAAAATGTATATACACAGCATCAACCT TTCCTACATGAAACCCTGGATCATCTCATCAAAGGAAGGCTTAAGGAAAACCTATATCCTTATTTAGGC CCCAGCACACTCAGAGACAGACCTCAGGATATCATTGTGTTTGTAATTGGAGGAGCCACCTATGAAGAG GCTCTAACAGTTTATAACCTGAACCGCACCACTCCTGGAGTGAGGATTGTCCTGGGAGGCACCACAGTG CACAACACGAAAAGTTTCCTAGAGGAAGTTCTGGCTTCTGGACTGCACAGCCGAAGCAAGGAGAGCTCT CAAGTCACATCAAGGTCAGCGAGCAGAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG238285
Blue=ORF Red=Cloning site Green=Tag(s) MNVVFAVKQYPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVK QVITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLRE VVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSK HVTVVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHS SNSLPGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQP FLHETLDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTV HNTKSFLEEVLASGLHSRSKESSQVTSRSASRR TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001279355 |
ORF Size | 1341 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001279355.1, NP_001266284.1 |
RefSeq Size | 2080 bp |
RefSeq ORF | 1344 bp |
Locus ID | 11311 |
Cytogenetics | 1q21.2 |
Protein Pathways | Endocytosis |
MW | 51.2 kDa |
Gene Summary | Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013] |
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