HCLS1 (NM_001292041) Human Tagged ORF Clone

CAT#: RG238298

HCLS1 (GFP-tagged) - Human hematopoietic cell-specific Lyn substrate 1 (HCLS1), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: HCLS1
• Synonyms: CTTNL; HS1; lckBP1; p75
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238298 representing NM_001292041.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGAAGTCTGTAGTGGGCCATGATGTGTCTGTTTCCGTGGAGACCCAGGGTGATGATTGGGACACA
  GATCCTGACTTTGTGAATGACATCTCTGAAAAGGAGCAACGATGGGGAGCCAAGACCATCGAGGGGTCT
  GGACGCACAGAACACATCAACATCCACCAGCTGAGGAACAAAGTATCAGAGGAGCATGATGTTCTCAGG
  AAGAAAGAGATGGAGTCAGGGCCCAAAGCATCCCATGGCTATGGAGGTCGGTTTGGAGTAGAAAGAGAC
  CGAATGGACAAGAGTGCAGTGGGCCATGAGTATGTTGCCGAGGTGGAGAAGCACTCTTCTCAGACGGAT
  GCTGCCAAAGGCTTTGGGGGCAAGTACGGAGTTGAGAGGGACAGGGCAGACAAGTCAGCAGTCGGCTTT
  GATTATAAAGGAGAAGTGGAGAAGCACACATCTCAGAAAGATTATGCCAAGGGCTTTGGTGGCCAGTAT
  GGAATCCAGAAGGACCGAGTGGATAAGAGCGCTGTCGGCTTCAATGAAATGGAGGCCCCGACCACAGCT
  TATAAGAAGACGACGCCCATAGAAGCCGCTTCTAGTGGTACCCGTGGGCTGAAGGCGAAATTTGAGTCC
  ATGGCTGAGGAGAAGAGGAAGCGAGAGGAAGAGGAGAAGGCACAGCAGGTGGCCAGGAGGCAACAGGAG
  CGAAAGGCTGTGACAAAGAGGAGCCCTGAGGCTCCACAGCCAGTGATAGCTATGGAAGAGCCAGCAGTA
  CCGGCCCCACTGCCCAAGAAAATCTCCTCAGAGGCCTGGCCTCCAGTTGGGACTCCTCCATCATCAGAG
  TCTGAGCCTGTGAGAACCAGCAGGGAACACCCAGTGCCCTTGCTGCCCATTAGGCAGACTCTCCCGGAG
  GACAATGAGGAGCCCCCAGCTCTGCCCCCTAGGACTCTGGAAGGCCTCCAGGTGGAGGAAGAGCCAGTG
  TACGAAGCAGAGCCTGAGCCTGAGCCCGAGCCTGAGCCCGAGCCTGAGAATGACTATGAGGACGTTGAG
  GAGATGGACAGGCATGAGCAGGAGGATGAACCAGAGGGGGACTATGAGGAGGTGCTCGAGCCTGAAGAT
  TCTTCTTTTTCTTCTGCTCTGGCTGGATCATCAGGCTGCCCGGCTGGGGCTGGGGCTGGGGCTGTGGCT
  CTGGGGATCTCAGCTGTGGCTCTATATGATTACCAAGGAGAGGGAAGTGATGAGCTTTCCTTTGATCCG
  GACGACGTAATCACTGACATTGAGATGGTGGACGAGGGCTGGTGGCGGGGACGTTGCCATGGCCACTTT
  GGACTCTTCCCTGCAAATTATGTCAAGCTTCTGGAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238298
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDVLR
  KKEMESGPKASHGYGGRFGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGFGGKYGVERDRADKSAVGF
  DYKGEVEKHTSQKDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGTRGLKAKFES
  MAEEKRKREEEEKAQQVARRQQERKAVTKRSPEAPQPVIAMEEPAVPAPLPKKISSEAWPPVGTPPSSE
  SEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLEGLQVEEEPVYEAEPEPEPEPEPEPENDYEDVE
  EMDRHEQEDEPEGDYEEVLEPEDSSFSSALAGSSGCPAGAGAGAVALGISAVALYDYQGEGSDELSFDP
  DDVITDIEMVDEGWWRGRCHGHFGLFPANYVKLLE
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001292041
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001292041.1, NP_001278970.1
• RefSeq Size: 1943 bp
• RefSeq ORF: 1350 bp
• Locus ID: 3059
• Cytogenetics: 3q13.33
• Protein Families: Transcription Factors
• Protein Pathways: Pathogenic Escherichia coli infection, Tight junction
• MW: 50.2 kDa
• Gene Summary: ''