NFIL3 (NM_001290000) Human Tagged ORF Clone

CAT#: RG238368

NFIL3 (GFP-tagged) - Human nuclear factor, interleukin 3 regulated (NFIL3), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: NFIL3
• Synonyms: E4BP4; IL3BP1; NF-IL3A; NFIL3A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238368 representing NM_001290000.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGCTGAGAAAAATGCAGACCGTCAAAAAGGAGCAGGCGTCTCTTGATGCCAGTAGCAATGTGGAC
  AAGATGATGGTCCTTAATTCTGCTTTAACGGAAGTGTCAGAAGACTCCACAACAGGTGAGGAGCTGCTT
  CTCAGTGAAGGAAGTGTGGGGAAGAACAAATCTTCTGCATGTCGGAGGAAACGGGAATTCATTCCTGAT
  GAAAAGAAAGATGCTATGTATTGGGAAAAAAGGCGGAAAAATAATGAAGCTGCCAAAAGATCTCGTGAG
  AAGCGTCGACTGAATGACCTGGTTTTAGAGAACAAACTAATTGCACTGGGAGAAGAAAACGCCACTTTA
  AAAGCTGAGCTGCTTTCACTAAAATTAAAGTTTGGTTTAATTAGCTCCACAGCATATGCTCAAGAGATT
  CAGAAACTCAGTAATTCTACAGCTGTGTACTTTCAAGATTACCAGACTTCCAAATCCAATGTGAGTTCA
  TTTGTGGACGAGCACGAACCCTCGATGGTGTCAAGTAGTTGTATTTCTGTCATTAAACACTCTCCACAA
  AGCTCGCTGTCCGATGTTTCAGAAGTGTCCTCAGTAGAACACACGCAGGAGAGCTCTGTGCAGGGAAGC
  TGCAGAAGTCCTGAAAACAAGTTCCAGATTATCAAGCAAGAGCCGATGGAATTAGAGAGCTACACAAGG
  GAGCCAAGAGATGACCGAGGCTCTTACACAGCGTCCATCTATCAAAACTATATGGGGAATTCTTTCTCT
  GGGTACTCACACTCTCCCCCACTACTGCAAGTCAACCGATCCTCCAGCAACTCCCCGAGAACGTCGGAA
  ACTGATGATGGTGTGGTAGGAAAGTCATCTGATGGAGAAGACGAGCAACAGGTCCCCAAGGGCCCCATC
  CATTCTCCAGTTGAACTCAAGCATGTGCATGCAACTGTGGTTAAAGTTCCAGAAGTGAATTCCTCTGCC
  TTGCCACACAAGCTCCGGATCAAAGCCAAAGCCATGCAGATCAAAGTAGAAGCCTTTGATAATGAATTT
  GAGGCCACGCAAAAACTTTCCTCACCTATTGACATGACATCTAAAAGACATTTCGAACTCGAAAAGCAT
  AGTGCCCCAAGTATGGTACATTCTTCTCTTACTCCTTTCTCAGTGCAAGTGACTAACATTCAAGATTGG
  TCTCTCAAATCGGAGCACTGGCATCAAAAAGAACTGAGTGGCAAAACTCAGAATAGTTTCAAAACTGGA
  GTTGTTGAAATGAAAGACAGTGGCTACAAAGTTTCTGACCCAGAGAACTTGTATTTGAAGCAGGGGATA
  GCAAACTTATCTGCAGAGGTTGTCTCACTCAAGAGACTTATAGCCACACAACCAATCTCTGCTTCAGAC
  TCTGGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238368
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MQLRKMQTVKKEQASLDASSNVDKMMVLNSALTEVSEDSTTGEELLLSEGSVGKNKSSACRRKREFIPD
  EKKDAMYWEKRRKNNEAAKRSREKRRLNDLVLENKLIALGEENATLKAELLSLKLKFGLISSTAYAQEI
  QKLSNSTAVYFQDYQTSKSNVSSFVDEHEPSMVSSSCISVIKHSPQSSLSDVSEVSSVEHTQESSVQGS
  CRSPENKFQIIKQEPMELESYTREPRDDRGSYTASIYQNYMGNSFSGYSHSPPLLQVNRSSSNSPRTSE
  TDDGVVGKSSDGEDEQQVPKGPIHSPVELKHVHATVVKVPEVNSSALPHKLRIKAKAMQIKVEAFDNEF
  EATQKLSSPIDMTSKRHFELEKHSAPSMVHSSLTPFSVQVTNIQDWSLKSEHWHQKELSGKTQNSFKTG
  VVEMKDSGYKVSDPENLYLKQGIANLSAEVVSLKRLIATQPISASDSG
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290000
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001290000.1, NP_001276929.1
• RefSeq Size: 2099 bp
• RefSeq ORF: 1389 bp
• Locus ID: 4783
• Cytogenetics: 9q22.31
• Protein Families: Transcription Factors
• MW: 51.5 kDa
• Gene Summary: 'The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]'