GBA3 (NM_001277225) Human Tagged ORF Clone

CAT#: RG238417

GBA3 (GFP-tagged) - Human glucosidase, beta, acid 3 (gene/pseudogene) (GBA3), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: GBA3
• Synonyms: CBG; CBGL1; GLUC; KLRP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238417 representing NM_001277225.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCCCTTTACACTTGCTTGGTGGAGATAACTCTTCCCTTCCATTTAGAAGAAAACGTAGGAGGCTGG
  GATGCAGATGGAAAAGGCCCTTGTGTCTGGGACACATTTACTCATCAGGGAGGAGAGAGAGTTTTCAAG
  AACCAGACTGGCGATGTAGCTTGTGGCAGCTACACTCTGTGGGAGGAAGATTTGAAATGTATCAAACAG
  CTTGGATTGACTCATTACCGCTTCTCTCTTTCCTGGTCACGTCTGTTACCTGATGGGACGACAGGTTTC
  ATCAACCAGAAAGGAATTGATTATTACAACAAGATCATCGATGATTTGTTAAAAAATGGGGTTACTCCC
  ATTGTGACCCTCTACCACTTTGATTTGCCTCAGACTTTAGAAGACCAAGGAGGTTGGTTGTCAGAGGCA
  ATCATTGAATCCTTTGACAAATATGCTCAGTTTTGCTTCAGTACCTTTGGGGATCGTGTCAAGCAGTGG
  ATCACCATAAATGAAGCTAATGTTCTTTCTGTGATGTCATATGACTTAGGTATGTTTCCTCCGGGTATC
  CCTCACTTTGGGACTGGAGGTTATCAGGCAGCTCATAATTTGATTAAGGCTCATGCCAGATCCTGGCAC
  AGCTATGATTCCTTATTTCGAAAAAAGCAGAAAGGTATGGTGTCTCTATCACTTTTTGCGGTCTGGTTG
  GAACCAGCAGATCCCAACTCAGTGTCTGACCAGGAAGCTGCTAAAAGAGCCATCACTTTCCATCTGGAT
  TTATTTGCTAAACCCATATTCATCGATGGTGATTATCCTGAAGTTGTCAAGTCTCAGATTGCCTCCATG
  AGTCAAAAGCAAGGCTATCCATCATCGAGGCTTCCAGAATTCACTGAAGAAGAGAAGAAAATGATCAAA
  GGCACTGCTGATTTTTTTGCTGTGCAATATTATACAACTCGCTTAATCAAGTACCAGGAGAACAAGAAA
  GGAGAACTAGGTATTCTCCAGGATGCGGAAATTGAATTTTTTCCAGATCCATCTTGGAAAAATGTGGAT
  TGGATCTACGTGGTACCATGGGGAGTATGTAAACTACTGAAATATATTAAGGATACATATAATAACCCT
  GTAATTTACATCACTGAGAATGGGTTTCCCCAGAGTGACCCAGCGCCTCTTGATGACACTCAACGCTGG
  GAGTATTTCAGACAAACATTTCAGGAACTGTTCAAAGCTATCCAACTTGATAAAGTCAATCTTCAAGTA
  TATTGTGCATGGTCTCTTCTGGATAACTTTGAGTGGAACCAGGGATACAGCAGCCGGTTTGGTCTCTTC
  CACGTTGATTTTGAAGACCCAGCTAGACCCCGAGTCCCTTACACATCGGCCAAGGAATATGCCAAGATC
  ATCCGAAACAATGGCCTTGAAGCACATCTG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238417
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSLYTCLVEITLPFHLEENVGGWDADGKGPCVWDTFTHQGGERVFKNQTGDVACGSYTLWEEDLKCIKQ
  LGLTHYRFSLSWSRLLPDGTTGFINQKGIDYYNKIIDDLLKNGVTPIVTLYHFDLPQTLEDQGGWLSEA
  IIESFDKYAQFCFSTFGDRVKQWITINEANVLSVMSYDLGMFPPGIPHFGTGGYQAAHNLIKAHARSWH
  SYDSLFRKKQKGMVSLSLFAVWLEPADPNSVSDQEAAKRAITFHLDLFAKPIFIDGDYPEVVKSQIASM
  SQKQGYPSSRLPEFTEEEKKMIKGTADFFAVQYYTTRLIKYQENKKGELGILQDAEIEFFPDPSWKNVD
  WIYVVPWGVCKLLKYIKDTYNNPVIYITENGFPQSDPAPLDDTQRWEYFRQTFQELFKAIQLDKVNLQV
  YCAWSLLDNFEWNQGYSSRFGLFHVDFEDPARPRVPYTSAKEYAKIIRNNGLEAHL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277225
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001277225.1, NP_001264154.1
• RefSeq Size: 2091 bp
• RefSeq ORF: 1413 bp
• Locus ID: 57733
• Cytogenetics: 4p15.2
• Protein Pathways: Cyanoamino acid metabolism, Starch and sucrose metabolism
• MW: 54.5 kDa
• Gene Summary: The protein encoded by this gene is an enzyme that can hydrolyze several types of glycosides. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2013]